AB250825

重组Anti-TMEM59L抗体[EPR14975] - BSA and Azide free

Anti-TMEM59L antibody [EPR14975] - BSA and Azide free

RabMAb
Recombinant
了解详情

Be the first to review this product! Submit a review

(2 Publications)

Rabbit Recombinant Monoclonal TMEM59L antibody. Carrier free. Suitable for WB and reacts with Human samples. Cited in 2 publications.

查看别名

BSMAP, C19orf4, TMEM59L, Transmembrane protein 59-like, Brain-specific membrane-anchored protein

2 Images

Supplier Data

Western blot - Anti-TMEM59L antibody [EPR14975] - BSA and Azide free (AB250825)

This data was developed using ab185227, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-TMEM59L antibody [EPR14975] (<a href='/products/primary-antibodies/tmem59l-antibody-epr14975-ab185227'>ab185227</a>) at 1/2000 dilution

All lanes:

293 cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 38 kDa

false

Supplier Data

Western blot - Anti-TMEM59L antibody [EPR14975] - BSA and Azide free (AB250825)

This data was developed using ab185227, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-TMEM59L antibody [EPR14975] (<a href='/products/primary-antibodies/tmem59l-antibody-epr14975-ab185227'>ab185227</a>) at 1/10000 dilution

All lanes:

Human cerebellum cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 38 kDa

false

不同偶联物与剂型 (1)

Unconjugated

Anti-TMEM59L antibody [EPR14975]

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR14975

亚型

IgG

不含载体蛋白

Yes

反应种属

Human

应用

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

style

left-column

反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }

style

left-column

产品详情

ab250825 is the carrier-free version of ab185227.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

style

left-column

性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

pH: 7.2 - 7.4 Constituents: PBS

运输条件

Blue Ice

储存信息

Do Not Freeze

style

left-column

补充信息

This supplementary information is collated from multiple sources and compiled automatically.

TMEM59L also known as Transmembrane Protein 59-Like is a protein with an approximate mass of 43 kDa. This protein is usually expressed in the brain with higher levels detected in regions like the cerebral cortex and hippocampus. TMEM59L is a type I transmembrane protein which means it spans the cellular membrane once and its N-terminal is inside the extracellular space. Its structure suggests a role in cellular signaling and membrane dynamics although its distinct functional domains are not entirely mapped yet.

Biological function summary

TMEM59L acts in neural differentiation and synaptic function. The protein influences neuronal development and may also play a role in synapse formation and maintenance. It is not known to be part of a larger multiprotein complex strictly but it interacts with several cellular components involved in neuronal plasticity. The presence of TMEM59L in neuronal tissue suggests that it contributes significantly to processes integral to cognitive functions.

Pathways

TMEM59L participates in cerebral processes involving cellular communication and neural integrity. It interacts within the Wnt signaling pathway which is important for neurite outgrowth and synaptic plasticity. This pathway involves other proteins such as Disheveled (DVL) and beta-catenin which further highlight the role of TMEM59L in neural connectivity and growth. In addition TMEM59L's interactions may influence pathways related to receptor trafficking connecting it to cytoskeleton-associated proteins.

TMEM59L links to neurodegenerative conditions including Alzheimer's disease and cognitive decline. In Alzheimer's disease irregularities in the expression or function of TMEM59L might disturb normal synaptic activity or neuronal health. This protein relates to beta-amyloid precursor protein (APP) which plays a role in disease pathology. Changes in TMEM59L could also affect neural plasticity leading to cognitive impairments suggesting that it might serve as a biomarker or therapeutic target in addressing such disorders.

style

left-column

style

left-column

产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

靶点信息

Modulates the O-glycosylation and complex N-glycosylation steps occurring during the Golgi maturation of APP. Inhibits APP transport to the cell surface and further shedding.

See full target information TMEM59L

style

left-column

文献 (2)

Recent publications for all applications. Explore the full list and refine your search

Journal of radiation research 64:833-841 PubMed37439405

2023

Expression of TMEM59L associated with radiosensitive in glioblastoma.

Applications

Unspecified application

Species

Unspecified reactive species

Dezhi Gao,Peng Wang,Lin Zhi,Shibin Sun,Xiaoguang Qiu,Yanwei Liu

The American journal of pathology 187:1686-1699 PubMed28618255

2017

Osteocyte Regulation of Receptor Activator of NF-κB Ligand/Osteoprotegerin in a Sheep Model of Osteoporosis.

Applications

Unspecified application

Species

Unspecified reactive species

Thaqif El Khassawna,Felix Merboth,Deeksha Malhan,Wolfgang Böcker,Diaa E S Daghma,Sabine Stoetzel,Stefanie Kern,Fathi Hassan,Dirk Rosenbaum,Judith Langenstein,Natali Bauer,Anja Schlagenhauf,Angela Rösen-Wolff,Felix Schulze,Markus Rupp,Dirk Hose,Anja Secklinger,Anita Ignatius,Hans-Joachim Wilke,Katrin S Lips,Christian Heiss

View all publications

style

left-column

style

left-column

style

right-column

Abcam Product Promise

我们致力于为您的研究提供高质量的试剂，为您科研的每一步提供支持。若我们的产品未能达到预期性能，我们向您提供 Abcam Product Promise 保障。

详情请参阅我们的条款与条件。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

重组Anti-TMEM59L抗体[EPR14975] - BSA and Azide free