AB250454

重组Anti-TMEM256抗体[EPR12842] - BSA and Azide free

Anti-TMEM256 antibody [EPR12842] - BSA and Azide free

RabMAb
Recombinant
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(1 Publication)

Rabbit Recombinant Monoclonal TMEM256 antibody. Carrier free. Suitable for IP, WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 1 publication.

查看别名

C17orf61, TMEM256, Transmembrane protein 256

4 Images

Flow Cytometry (Intracellular) - Anti-TMEM256 antibody [EPR12842] - BSA and Azide free (AB250454)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-TMEM256 antibody [EPR12842] - BSA and Azide free (AB250454)

This data was developed using ab181239, the same antibody clone in a different buffer formulation.

Intracellular flow cytometric analysis of A549 cells (paraformaldehyde-fixed, 2%) labeling TMEM256 with ab181239 at 1/200 dilution (red) or a rabbit IgG (negative) (green), followed by Goat anti rabbit IgG (FITC) secondary at 1/150 dilution.

Supplier Data

Immunoprecipitation - Anti-TMEM256 antibody [EPR12842] - BSA and Azide free (AB250454)

This data was developed using ab181239, the same antibody clone in a different buffer formulation.

Western blot analysis of immunoprecipitation pellet from A549 cell lysate immunoprecipitated using ab181239 at 1/50 dilution.
Secondary : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution.

All lanes:

Immunoprecipitation - Anti-TMEM256 antibody [EPR12842] (<a href='/products/primary-antibodies/tmem256-antibody-epr12842-ab181239'>ab181239</a>)

Predicted band size: 12 kDa

false

Supplier Data

Western blot - Anti-TMEM256 antibody [EPR12842] - BSA and Azide free (AB250454)

This data was developed using ab181239, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-TMEM256 antibody [EPR12842] (<a href='/products/primary-antibodies/tmem256-antibody-epr12842-ab181239'>ab181239</a>) at 1/2000 dilution

Lane 1:

Human fetal liver lysate at 20 µg

Lane 2:

Human fetal kidney lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab136636'>ab136636</a>) at 1/500 dilution

Predicted band size: 12 kDa

false

Supplier Data

Western blot - Anti-TMEM256 antibody [EPR12842] - BSA and Azide free (AB250454)

This data was developed using ab181239, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-TMEM256 antibody [EPR12842] (<a href='/products/primary-antibodies/tmem256-antibody-epr12842-ab181239'>ab181239</a>) at 1/10000 dilution

Lane 1:

A549 cell lysate at 20 µg

Lane 2:

Y79 cell lysate at 20 µg

Lane 3:

LnCaP cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 12 kDa

false

不同偶联物与剂型 (1)

Unconjugated

Anti-TMEM256 antibody [EPR12842]

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR12842

亚型

IgG

不含载体蛋白

Yes

反应种属

Human

应用

WB, Flow Cyt (Intra), IP

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Mouse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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产品详情

ab250454 is the carrier-free version of ab181239.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification

存储溶液

pH: 7.2 - 7.4 Constituents: PBS

运输条件

Blue Ice

储存信息

Do Not Freeze

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

TMEM256 also known as Transmembrane Protein 256 is an integral membrane protein with a mass of approximately 18 kDa. It localizes to the cell membrane where it spans the lipid bilayer with multiple transmembrane domains. Expression of TMEM256 is noted in several tissues with higher expression observed in the brain and testis. This suggests a role linked to specific tissue functions possibly related to signaling processes or transport mechanisms across the membrane.

Biological function summary

TMEM256 appears significant in modulating membrane potential and maintaining homeostasis. Though not part of a well-defined complex the protein's characteristics suggest involvement in maintaining cellular ion gradients. This may implicate TMEM256 in key cellular activities such as neuron signaling or other electrically excitable tissue functions. Its role suggests interactions with other membrane proteins which could further modulate its activity or be involved in cellular responses to the external environment.

Pathways

TMEM256 influences cellular signaling and membrane transport mechanisms. It is implicated in ion transport pathways and may interact with proteins like sodium-potassium pumps contributing to maintaining membrane potential. Additionally TMEM256 likely plays a role in neural communication pathways potentially interacting with proteins essential in synaptic function such as neurotransmitter receptors or ion channels suggesting coordination in neuron signaling and rapid response systems.

TMEM256 has links to neurological conditions and reproductive health issues. Aberrant expression or mutations in TMEM256 may connect to neurological disorders potentially through its role in modulating ion gradients and neuron excitability. Another aspect is the possible involvement in reproductive disorders due to its expression patterns in testis. The protein could interact with other disease-related proteins such as ion channels or transporters which are known to be associated with both neurological and reproductive health.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

See full target information TMEM256

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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Evidence-based complementary and alternative medicine : eCAM 2022:7757833 PubMed36091585

2022

Esculentoside A Alleviates Intestinal Dysmotility in Ulcerative Colitis by Regulating HS/CSE and NO/nNOS Systems.

Applications

Unspecified application

Species

Unspecified reactive species

Ying Liu,Wenhua Wei,Shiwei Liang,Haicheng Fang,Jie Cao

View all publications

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重组Anti-TMEM256抗体[EPR12842] - BSA and Azide free