Anti-TMEM25抗体(ab121362)
Key features and details
- Rabbit polyclonal to TMEM25
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Human
- Isotype: IgG
概述
-
产品名称
Anti-TMEM25抗体 -
描述
兔多克隆抗体to TMEM25 -
宿主
Rabbit -
经测试应用
适用于: ICC/IF, IHC-P, WBmore details -
种属反应性
与反应: Human -
免疫原
antigen, corresponding to amino acids 262-364 of Human TMEM25 (Uniprot: Q86YD3)
-
阳性对照
- Human duodenum tissue and Liver lysate.
-
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
-
Compatible Secondaries
-
Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab121362于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF |
Use a concentration of 0.25 - 2 µg/ml.
|
|
| IHC-P |
1/20 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
|
| WB |
1/250 - 1/500.
|
| 说明 |
|---|
|
ICC/IF
Use a concentration of 0.25 - 2 µg/ml. |
|
IHC-P
1/20 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
|
WB
1/250 - 1/500. |
靶标
-
序列相似性
Contains 1 Ig-like (immunoglobulin-like) domain. -
细胞定位
Secreted and Cell membrane. - Information by UniProt
-
数据库链接
- Entrez Gene: 84866 Human
- Omim: 613934 Human
- SwissProt: Q86YD3 Human
- Unigene: 564188 Human
-
别名
- 0610039J01Rik antibody
- FLJ14399 antibody
- Tmem25 antibody
see all
图片
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMEM25 antibody (ab121362)
Immunohistochemical analysis of human duodenum tissue labelled for TMEM25 using ab121362 at a dilution of 1/50. Positive cytoplasmic staining in glandular cells.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMEM25 antibody (ab121362)Immunohistochemical analysis of human cerebellum tissue labelled for TMEM25 using ab121362 at a dilution of 1/50. Positive cytoplasmic staining in granular layer cells.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMEM25 antibody (ab121362)Immunohistochemical analysis of human kidney tissue labelled for TMEM25 using ab121362 at a dilution of 1/50. Positive cytoplasmic staining in human tubule cells.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMEM25 antibody (ab121362)Immunohistochemical analysis of human prostate tissue labelled for TMEM25 using ab121362 at a dilution of 1/50. Positive cytoplasmic and membranous staining in human glandular cells.
-
Western blot - Anti-TMEM25 antibody (ab121362)All lanes : Anti-TMEM25 antibody (ab121362) at 1/250 dilution
Lane 1 : RT-4 lysate
Lane 2 : U-251 MG lysate
Lane 3 : Human Plasma lysate
Lane 4 : Liver lysate
Lane 5 : Tonsil lysate -
Immunocytochemistry/ Immunofluorescence - Anti-TMEM25 antibody (ab121362)Immunofluorescent staining of human cell line A-431 shows positivity in vesicles.
实验方案
数据表及文件
-
SDS download
-
Datasheet download
文献 (0)
ab121362 尚未被引用在任何文献中。