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Anti-TMEM16A抗体(ab72984)

Reviews (1)Q&A (2)References (9)
Immunocytochemistry/ Immunofluorescence - Anti-TMEM16A antibody (ab72984)

    Key features and details

    • Rabbit polyclonal to TMEM16A
    • Suitable for: ICC/IF
    • Reacts with: Human
    • Isotype: IgG

    概述

    • 产品名称

      Anti-TMEM16A抗体
      参阅全部 TMEM16A 一抗

    • 描述

      兔多克隆抗体to TMEM16A

    • 宿主

      Rabbit

    • 特异性

      This antibody reacts specifically with human 114 kDa TMEM16A protein.

    • 经测试应用

      适用于: ICC/IFmore details

    • 种属反应性

      与反应: Human

    • 免疫原

      Synthetic peptide corresponding to Human TMEM16A aa 700-800 (internal sequence).

    • 常规说明

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    性能

    • 形式

      Liquid

    • 存放说明

      Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

    • 存储溶液

      Constituent: Whole serum
    • Concentration information loading...

    • 纯度

      Whole antiserum

    • 克隆

      多克隆

    • 同种型

      IgG

    • 研究领域

    应用

    The Abpromise guarantee

    Abpromise™承诺保证使用ab72984于以下的经测试应用

    “应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

    应用 Ab评论 说明
    ICC/IF
    1/1000.
    说明
    ICC/IF
    1/1000.

    靶标

    • 功能

      Acts as a calcium-activated chloride channel. Required for normal tracheal development.

    • 组织特异性

      Broadly expressed with higher levels in liver and skeletal muscle.

    • 序列相似性

      Belongs to the anoctamin family.

    • 结构域

      The region spanning the fifth and sixth transmembrane domains probably forms the pore-forming region.

    • 细胞定位

      Cell membrane. Cytoplasm.
    • Information by UniProt

    • 数据库链接

      • 别名

        • ANO 1 antibody
        • ANO1 antibody
        • ANO1_HUMAN antibody
        • Anoctamin 1 antibody
        • Anoctamin 1 calcium activated chloride channel antibody
        • Anoctamin-1 antibody
        • Anoctamin1 antibody
        • Ca2+ activated Cl- channel antibody
        • Calcium Activated Chloride Channel antibody
        • Discovered on gastrointestinal stromal tumors protein 1 antibody
        • DOG 1 antibody
        • DOG1 antibody
        • FLJ10261 antibody
        • Membrane protein antibody
        • Oral cancer overexpressed 2 antibody
        • Oral cancer overexpressed protein 2 antibody
        • ORAOV 2 antibody
        • ORAOV2 antibody
        • TAOS 2 antibody
        • TAOS2 antibody
        • TMEM 16A antibody
        • TMEM16A antibody
        • Transmembrane protein 16A (eight membrane spanning domains) antibody
        • Transmembrane protein 16A antibody
        • Tumor amplified and overexpressed sequence 2 antibody
        • Tumor-amplified and overexpressed sequence 2 antibody
        see all

      图片

      • Immunocytochemistry/ Immunofluorescence - Anti-TMEM16A antibody (ab72984)
        Immunocytochemistry/ Immunofluorescence - Anti-TMEM16A antibody (ab72984)
        ICC/IF image of ab72984 stained Hek293 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab72984, 1/1000 dilution) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

      文献 (9)

      发表研究结果有使用 ab72984？请让我们知道，以便我们可以引用本数据表中的参考文章。

      ab72984 被引用在 9 文献中.

      • Heng J  et al. miR-106a Targets Anoctamin 1 (ANO1) to Regulate Lipopolysaccharide (LPS)-Induced Inflammatory Response in Macrophages. Med Sci Monit 26:e922479 (2020). PubMed: 33037174
      • Chen D  et al. ANO1 in urethral SMCs contributes to sex differences in urethral spontaneous tone. Am J Physiol Renal Physiol 319:F394-F402 (2020). PubMed: 32686521
      • Tong JJ  et al. Calcium-Activated Chloride Channels in Newly Differentiating Mouse Lens Fiber Cells and Their Role in Volume Regulation. Invest Ophthalmol Vis Sci 60:1621-1629 (2019). PubMed: 30995319
      • Banerjee J  et al. Regulatory Roles of Anoctamin-6 in Human Trabecular Meshwork Cells. Invest Ophthalmol Vis Sci 58:492-501 (2017). WB . PubMed: 28125837
      • Crutzen R  et al. Anoctamin 1 (Ano1) is required for glucose-induced membrane potential oscillations and insulin secretion by murine ß-cells. Pflugers Arch 468:573-91 (2016). PubMed: 26582426
      • Davis AJ  et al. Potent vasorelaxant activity of the TMEM16A inhibitor T16A(inh) -A01. Br J Pharmacol 168:773-84 (2013). PubMed: 22946562
      • Wang M  et al. Downregulation of TMEM16A calcium-activated chloride channel contributes to cerebrovascular remodeling during hypertension by promoting basilar smooth muscle cell proliferation. Circulation 125:697-707 (2012). WB ; Rat . PubMed: 22215857
      • Hanzu FA  et al. Expression of TMEM16A and SLC4A4 in human pancreatic islets. Cell Physiol Biochem 29:61-4 (2012). PubMed: 22415075
      • Davis AJ  et al. Expression profile and protein translation of TMEM16A in murine smooth muscle. Am J Physiol Cell Physiol 299:C948-59 (2010). IHC-Fr ; Mouse . PubMed: 20686072

      客户评价及客户问答

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      1-3 of 3 Abreviews or Q&A

      Western blot abreview for Anti-TMEM16A antibody

       Average
      Abreviews
      abreview image
      Application
      Western blot
      Sample
      Rat Tissue lysate - whole (arterial homogenate)
      Loading amount
      50 µg
      Specification
      arterial homogenate
      Gel Running Conditions
      Reduced Denaturing
      Blocking step
      Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
      Read More
      The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

      Abcam user community

      Verified customer

      提交于 Sep 03 2010

      Question

      Attached please find the amino acid sequences of interest of zebrafish (a total of 3). Thanks in advance!

      Kind Regards,

      Read More
      Answer

      Thank you for your reply and for kindly providing the word document. This has made conducting the alignments much more straight forward.

      I am sorry to confirm that as far as we are aware,none of our TMEM16A antibodieshas been tested with samples from Zebrafish. All tested species covered by the 6 month guarantee are stated on our datasheets, and these are updated as soon as any new information is brought to our attention.

      I have obtained the immungoen sequences for all our TMEM16A antibodies and checked the alignment of these with the 3 Zebrafish sequences you provided.

      Regrettably, for most of these the alignments are too low for us to suggest they may detect in Zebrafish. However, there is one with reasonable alignment that you may like to consider trying. I can recmomend to review the online datasheet for further information.

      ab84115
      https://www.abcam.com/TMEM16A-antibody-ab84115.html (or use the following: https://www.abcam.com/TMEM16A-antibody-ab84115.html).

      Immunogen: ################

      Alignment with Zebrafish sequences provided:
      Sequence 1: 68%
      Sequence 2: 68%
      Sequence 3: 72%

      We recommend alignment should be over 85% to predict that an antibody will detect. It isstill possible that with 68-72% alignment therecould be some crossreactivity. However, even when alignmentis very highwe cannot guarantee this without further testing.


      If you would like to test the ab84115 in Zebrafish, please contact me again by replying to this message prior to the purchase as you may be eligible for our testing discount program.

      Otherwise, we like to encourage all of our customers to submit an Abreview via the online product datasheet. We always appreciate customer feedback, whether positive or negative, and we make all product information available to researchers. Each Abreview earns Abpoints that can be used for discounts on future purchases or rewards such as Amazon.com gift certificates.

      To find out more about our Abreview system, please see the following link:

      https://www.abcam.com/abreviews

      I hope this information is helpful. Please do not hesitate to contact me for any further advice or information.

      Read More

      Question

      ab72984 gives no signal in WB on human samples

      Read More
      Answer

      Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.
      I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement with the order number ***.
      To check the status of the order please contact our Customer Service team and reference this number.
      Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.
      I wish you the best of luck with your research.

      Read More

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com