Key features and details
- Rabbit polyclonal to TMEM16A
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术，可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
参阅全部 TMEM16A 一抗
经测试应用适用于: IHC-Pmore details
预测可用于: Mouse, Rat
Synthetic peptide within Human TMEM16A aa 400-500. The exact sequence is proprietary.
Database link: Q5XXA6
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存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA
Concentration information loading...
纯度Immunogen affinity purified
功能Acts as a calcium-activated chloride channel. Required for normal tracheal development.
组织特异性Broadly expressed with higher levels in liver and skeletal muscle.
序列相似性Belongs to the anoctamin family.
结构域The region spanning the fifth and sixth transmembrane domains probably forms the pore-forming region.
细胞定位Cell membrane. Cytoplasm.
- Information by UniProt
- ANO 1 antibody
- ANO1 antibody
- ANO1_HUMAN antibody
ab53212 (2µg/ml) staining TMEM16A in human liver (left panel) using an automated system (DAKO Autostainer Plus). Using this protocol there is strong staining of hepatocyte cell membrane.
Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human gastrointestinal stromal tumor labeling TMEM16A with ab53212.
ab53212 被引用在 101 文献中.
- Li XL et al. Blockade of TMEM16A protects against renal fibrosis by reducing intracellular Cl- concentration. Br J Pharmacol 179:3043-3060 (2022). PubMed: 34961937
- Zhang Y et al. TMEM16A Plays an Insignificant Role in Myocardium Remodeling but May Promote Angiogenesis of Heart During Pressure-overload. Front Physiol 13:897619 (2022). PubMed: 35711304
- Su S et al. A Mathematical Model of Salivary Gland Duct Cells. Bull Math Biol 84:84 (2022). PubMed: 35799078
- Kawata N et al. Increased TMEM16A-Mediated Ca2+-Activated Cl- Currents in Portal Vein Smooth Muscle Cells of Caveolin 1-Deficient Mice. Biol Pharm Bull 45:1692-1698 (2022). PubMed: 35989294
- Lin JM et al. Sociosexual behavior requires both activating and repressive roles of Tfap2e/AP-2ε in vomeronasal sensory neurons. Elife 11:N/A (2022). PubMed: 36111787