Anti-TMEM147抗体(ab97624)
Key features and details
- Rabbit polyclonal to TMEM147
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
概述
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产品名称
Anti-TMEM147抗体
参阅全部 TMEM147 一抗 -
描述
兔多克隆抗体to TMEM147 -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-P, ICC/IFmore details -
种属反应性
与反应: Mouse, Human
预测可用于: Rat, Cow, Zebrafish
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免疫原
Synthetic peptide corresponding to a region within amino acids 87 and 101 of Human TMEM147.
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阳性对照
- WB: Raji or NIH 3T3 cell lysate; ICC/IF: A431 cells; IHC-P: NCI-N87 xenograft.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab97624于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/500 - 1/3000. Predicted molecular weight: 25 kDa.
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| IHC-P |
1/100 - 1/500.
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| ICC/IF |
1/100 - 1/200.
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| 说明 |
|---|
|
WB
1/500 - 1/3000. Predicted molecular weight: 25 kDa. |
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IHC-P
1/100 - 1/500. |
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ICC/IF
1/100 - 1/200. |
靶标
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细胞定位
Endoplasmic reticulum membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 10430 Human
- Entrez Gene: 69804 Mouse
- Entrez Gene: 292792 Rat
- Entrez Gene: 436757 Zebrafish
- SwissProt: Q9BVK8 Human
- SwissProt: Q9CQG6 Mouse
- SwissProt: Q2TA63 Rat
- SwissProt: Q6DGL7 Zebrafish
see all -
别名
- NIFIE 14 antibody
- Protein NIFIE 14 antibody
- Seven transmembrane domain protein antibody
see all
图片
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Western blot - Anti-TMEM147 antibody (ab97624)Anti-TMEM147 antibody (ab97624) at 1/1500 dilution + Raji whole cell lysate at 30 µg
Predicted band size: 25 kDa
12% SDS PAGE -
Western blot - Anti-TMEM147 antibody (ab97624)Anti-TMEM147 antibody (ab97624) at 1/1000 dilution + NIH 3T3 whole cell lysate at 30 µg
Predicted band size: 25 kDa
12% SDS PAGE -
Immunocytochemistry/ Immunofluorescence - Anti-TMEM147 antibody (ab97624)1/200 ab97624 staining TMEM147 in paraformaldehyde-fixed A431 cells by immunofluorescence.
The lower image was co-stained with Hoechst 33342. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMEM147 antibody (ab97624)1/500 ab97624 staining TMEM147 in paraffin-embedded NCI-N87 xenograft by immunohistochemistry.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab97624 尚未被引用在任何文献中。