Anti-TMEM109/Mitsugumin-23抗体(ab121349)
Key features and details
- Rabbit polyclonal to TMEM109/Mitsugumin-23
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-TMEM109/Mitsugumin-23抗体
参阅全部 TMEM109/Mitsugumin-23 一抗 -
描述
兔多克隆抗体to TMEM109/Mitsugumin-23 -
宿主
Rabbit -
经测试应用
适用于: IHC-P, WB, ICC/IFmore details -
种属反应性
与反应: Human -
免疫原
Recombinant fragment corresponding to Human TMEM109/Mitsugumin-23 aa 212-242.
Sequence:LEAKVRGLERQVEELRWRQRRAAKGARSVEE
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab121349于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P |
1/1000. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
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| WB | (1) |
Use a concentration of 0.04 - 0.4 µg/ml.
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| ICC/IF | (1) |
Use a concentration of 0.25 - 2 µg/ml.
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| 说明 |
|---|
|
IHC-P
1/1000. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol. |
|
WB
Use a concentration of 0.04 - 0.4 µg/ml. |
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ICC/IF
Use a concentration of 0.25 - 2 µg/ml. |
靶标
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细胞定位
Nucleus outer membrane. Endoplasmic reticulum membrane. Sarcoplasmic reticulum membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 79073 Human
- SwissProt: Q9BVC6 Human
- Unigene: 13662 Human
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别名
- Mg23 antibody
- Mitsugumin-23 antibody
- TM109_HUMAN antibody
see all
图片
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Western blot - Anti-TMEM109/Mitsugumin-23 antibody (ab121349)All lanes : Anti-TMEM109/Mitsugumin-23 antibody (ab121349) at 1/250 dilution
Lane 1 : RT4 lysate
Lane 2 : U 251 MG lysate
Lane 3 : Human plasma lysate
Lane 4 : Human liver lysate
Lane 5 : Human tonsil lysate
Developed using the ECL technique. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMEM109/Mitsugumin-23 antibody (ab121349)ab121349, at 1/1000, staining TMEM109/Mitsugumin-23 in Paraffin-embedded Human pancreas tissue by Immunohistochemistry.
Note: strong cytoplasmic positivity in exocrine glandular cells. -
Immunocytochemistry/ Immunofluorescence - Anti-TMEM109/Mitsugumin-23 antibody (ab121349)ab121349 at 4 µg/ml staining TMEM109/Mitsugumin-23 in PFA/Triton X-100 fixed/permeabilized U 251 MG cells by Immunofluorescence (green).
Note: positivity in nucleus but not nucleoli, nuclear membrane and cytoplasm.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab121349 尚未被引用在任何文献中。