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Anti-TLR9抗体- Aminoterminal end (ab53396)

Submit a review Q&A (1)References (6)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TLR9 antibody - Aminoterminal end (ab53396)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TLR9 antibody - Aminoterminal end (ab53396)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TLR9 antibody - Aminoterminal end (ab53396)

Key features and details

  • Goat polyclonal to TLR9 - Aminoterminal end
  • Suitable for: IHC-P
  • Reacts with: Mouse, Human
  • Isotype: IgG

选择批间可重复性更高的重组抗体

Product image
Anti-TLR9 antibody [EPR14964-2] (ab187148)
  • 研究可靠 —— 各批次间结果一致且可重复
  • 长期批量供应 —— 采用重组技术，可实现快速生产
  • 首次实验即可成功 —— 经过大量验证确认了特异性
  • 符合伦理标准 —— 产品不含动物成分

概述

  • 产品名称

    Anti-TLR9抗体- Aminoterminal end
    参阅全部 TLR9 一抗

  • 描述

    山羊多克隆抗体to TLR9 - Aminoterminal end

  • 宿主

    Goat

  • 经测试应用

    适用于: IHC-Pmore details

  • 种属反应性

    与反应: Mouse, Human
    预测可用于: Chimpanzee

  • 免疫原

    31 amino acid (aa) synthetic peptide

    SLKYNNLTVVPRNLPSSLEYLLLSYNRIVKL

    corresponding to aa 205-235 of the N-terminal domain of Human TLR9
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • 常规说明

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab53396于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
IHC-P
Use at an assay dependent concentration.
说明
IHC-P
Use at an assay dependent concentration.

靶标

  • 功能

    Key component of innate and adaptive immunity. TLRs (Toll-like receptors) control host immune response against pathogens through recognition of molecular patterns specific of microorganisms. TLR9 is a nucleotide-sensing TLR which is activated by unmethylated cytidine-phosphate-guanosine (CpG) dinucleotides. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response.

  • 组织特异性

    Highly expressed in spleen, lymph node, tonsil and peripheral blood leukocytes, especially in plasmacytoid pre-dendritic cells. Levels are much lower in monocytes and CD11c+ immature dendritic cells. Also detected in lung and liver.

  • 序列相似性

    Belongs to the Toll-like receptor family.
    Contains 26 LRR (leucine-rich) repeats.
    Contains 1 TIR domain.

  • 细胞定位

    Endoplasmic reticulum membrane. Endosome. Lysosome. Cytoplasmic vesicle > phagosome. Relocalizes from endoplasmic reticulum to endosome and lysosome upon stimulation with agonist.
  • Information by UniProt

  • 数据库链接

    • 别名

      • CD 289 antibody
      • CD289 antibody
      • TLR 9 antibody
      • TLR9 antibody
      • TLR9_HUMAN antibody
      • Toll like receptor 9 antibody
      • Toll like receptor 9 isoform A precursor antibody
      • Toll like receptor 9 isoform B antibody
      • Toll-like receptor 9 antibody
      see all

    图片

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TLR9 antibody - Aminoterminal end (ab53396)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TLR9 antibody - Aminoterminal end (ab53396)
      IH on paraffin section of human spleen using ab53396 at a concentration of 1/250.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TLR9 antibody - Aminoterminal end (ab53396)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TLR9 antibody - Aminoterminal end (ab53396)
      IH on paraffin section of human tonsil using ab53396 at a concentration of 1/250.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TLR9 antibody - Aminoterminal end (ab53396)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TLR9 antibody - Aminoterminal end (ab53396)
      IH on paraffin section of mouse spleen using ab53396 at a concentration of 1/250.

    文献 (6)

    发表研究结果有使用 ab53396？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab53396 被引用在 6 文献中.

    • Zheng H  et al. Depletion of Toll-Like Receptor-9 Attenuates Renal Tubulointerstitial Fibrosis After Ischemia-Reperfusion Injury. Front Cell Dev Biol 9:641527 (2021). PubMed: 33644078
    • Zheng H  et al. Hydroxychloroquine Inhibits Macrophage Activation and Attenuates Renal Fibrosis After Ischemia-Reperfusion Injury. Front Immunol 12:645100 (2021). PubMed: 33936063
    • Lai JH  et al. Mitochondrial CMPK2 mediates immunomodulatory and antiviral activities through IFN-dependent and IFN-independent pathways. iScience 24:102498 (2021). PubMed: 34142025
    • O'Sullivan KM  et al. Intrarenal Toll-like receptor 4 and Toll-like receptor 2 expression correlates with injury in antineutrophil cytoplasmic antibody-associated vasculitis. Am J Physiol Renal Physiol 315:F1283-F1294 (2018). PubMed: 29923769
    • Lai JH  et al. Infection with the dengue RNA virus activates TLR9 signaling in human dendritic cells. EMBO Rep 19:N/A (2018). PubMed: 29880709
    • Thiyagarajan D  et al. Silencing of renal DNaseI in murine lupus nephritis imposes exposure of large chromatin fragments and activation of Toll like receptors and the Clec4e. PLoS One 7:e34080 (2012). IHC-P, IHC-Fr ; Human, Mouse . PubMed: 22479529

    客户评价及客户问答

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    Question

    Lot number: GR4591-1
    Inquiry: Hello, we tested this antibody (1/250) for TLR9 detection on FFPE tissue sections under different antigen retrieval conditions : 10mM citrate buffer pH6, EDTA pH8.8, 1% trypsin and pepsin (ready-to-use commercial solution). The secondary antibody is a donkey anti-goat IgG - HRP and we used the Tyramide amplification system. All conditions without success. Could you give us some indications to become successful with this antibody ? Thank you for your answer Best regards

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    Answer

    I am sorry to hear that you have been experiencing problems using this product in the application that you wish.

    In order to assess the quality of our products I would ask that you complete a brief questionnaire relating to the application used. Often it is possible to make suggestions that may help resolve problems experienced using a particular product.

    As our Abpromise indicates, in the event that a product is not functioning in the applications/species cited on the product data sheet (and the problem has been reported within 6 months of purchase) we will happily offer a credit note/refund to the value of the product purchased.

    All our customer feedback, including complaints are monitored weekly by our in house technical support team. If a product is at fault the technical support team will consider removing the product from our catalogue in order to avoid future customer inconvenience.

    Please could you provide some further details of the protocol used and complete the following form (attached as a word document). It would be much appreciated if you could attach an image to the response.

    Thank you for your understanding and co-operation in this matter. I look forward to hearing from you soon and resolving this issue as soon as possible.

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