Anti-TLR9抗体[26C593.2] (ab134368)
Key features and details
- Mouse monoclonal [26C593.2] to TLR9
- Suitable for: WB, Flow Cyt (Intra), ICC/IF, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG1
概述
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产品名称
Anti-TLR9抗体[26C593.2]
参阅全部 TLR9 一抗 -
描述
小鼠单克隆抗体[26C593.2] to TLR9 -
宿主
Mouse -
经测试应用
适用于: WB, Flow Cyt (Intra), ICC/IF, IHC-Pmore details -
种属反应性
与反应: Mouse, Rat, Human
预测可用于: Chimpanzee, Cynomolgus monkey, Gorilla, Orangutan -
免疫原
Synthetic peptide corresponding to Human TLR9 aa 268-284 conjugated to keyhole limpet haemocyanin. AAF78037
Sequence:CPRHFPQLHPDTFSHLS
Database link: Q9NR96 -
阳性对照
- WB: Human peripheral blood mononuclear cells (PBMCs). Human, mouse and rat intestine tissue lysates. Flow Cyt (Intra): Ramos and PBMC cells. IHC-P: Human spleen tissue. ICC/IF: A549 cells.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at 4°C (up to 6 months). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.05% Sodium azide
Constituents: 0.05% BSA, PBS -
Concentration information loading...
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纯度
Protein G purified -
克隆
单克隆 -
克隆编号
26C593.2 -
同种型
IgG1 -
轻链类型
kappa -
研究领域
相关产品
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab134368于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 120 kDa (predicted molecular weight: 115 kDa).
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Flow Cyt (Intra) |
Use 0.1-2µg for 106 cells.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
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ICC/IF |
Use at an assay dependent concentration.
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IHC-P |
Use a concentration of 5 µg/ml.
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说明 |
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WB
Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 120 kDa (predicted molecular weight: 115 kDa). |
Flow Cyt (Intra)
Use 0.1-2µg for 106 cells. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
ICC/IF
Use at an assay dependent concentration. |
IHC-P
Use a concentration of 5 µg/ml. |
靶标
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功能
Key component of innate and adaptive immunity. TLRs (Toll-like receptors) control host immune response against pathogens through recognition of molecular patterns specific of microorganisms. TLR9 is a nucleotide-sensing TLR which is activated by unmethylated cytidine-phosphate-guanosine (CpG) dinucleotides. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. -
组织特异性
Highly expressed in spleen, lymph node, tonsil and peripheral blood leukocytes, especially in plasmacytoid pre-dendritic cells. Levels are much lower in monocytes and CD11c+ immature dendritic cells. Also detected in lung and liver. -
序列相似性
Belongs to the Toll-like receptor family.
Contains 26 LRR (leucine-rich) repeats.
Contains 1 TIR domain. -
细胞定位
Endoplasmic reticulum membrane. Endosome. Lysosome. Cytoplasmic vesicle > phagosome. Relocalizes from endoplasmic reticulum to endosome and lysosome upon stimulation with agonist. - Information by UniProt
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数据库链接
- Entrez Gene: 54106 Human
- Entrez Gene: 81897 Mouse
- Entrez Gene: 338457 Rat
- Omim: 605474 Human
- SwissProt: Q9NR96 Human
- SwissProt: Q9EQU3 Mouse
- Unigene: 87968 Human
- Unigene: 44889 Mouse
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别名
- CD 289 antibody
- CD289 antibody
- TLR 9 antibody
see all
图片
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All lanes : Anti-TLR9 antibody [26C593.2] (ab134368) at 3 µg/ml
Lane 1 : Human peripheral blood mononuclear cells (PBMCs)
Lane 2 : Human intestine tissue lysate
Lane 3 : Mouse intestine tissue lysate
Lane 4 : Rat intestine tissue lysate
Lysates/proteins at 40 µg per lane.
Secondary
All lanes : Goat anti-mouse HRP conjugate
Predicted band size: 115 kDa
Observed band size: 120 kDa why is the actual band size different from the predicted? -
Immunohistochemical analysis of TLR9 in frozen sections of A549 (Human lung carcinoma cell line) cells, using ab134368 at a 1/100 dilution.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TLR9 antibody [26C593.2] (ab134368)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human spleen tissue labelling TLR9 with ab134368 at 5 µg/ml.
Staining was enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 minutes followed by cooling at room temperature for 20 minutes.
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Flow Cytometric analysis of TLR9 in Ramos (Human Burkitt's lymphoma cell line) cells labelled with ab134368 at 0.1 µg (pink), no antibody (blue) or isotype control antibody (green). Goat anti-mouse IgG FITC secondary was used.
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Flow Cytometric analysis of TLR9 in human PBMC cells labelled with ab134368 at 0.5 µg (red) or an isotype control antibody (green).
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (22)
ab134368 被引用在 22 文献中.
- Miyahara Y et al. Toll-like receptor 9-positive plasmacytoid dendritic cells promote Th17 immune responses in oral lichen planus stimulated by epithelium-derived cathepsin K. Sci Rep 13:19320 (2023). PubMed: 37935734
- Del Padre M et al. Dual stimulation by autoantigen and CpG fosters the proliferation of exhausted rheumatoid factor-specific CD21low B cells in hepatitis C virus-cured mixed cryoglobulinemia. Front Immunol 14:1094871 (2023). PubMed: 36845129
- Zhang J et al. Mutual promotion of mitochondrial fission and oxidative stress contributes to mitochondrial-DNA-mediated inflammation and epithelial-mesenchymal transition in paraquat-induced pulmonary fibrosis. World J Emerg Med 14:209-216 (2023). PubMed: 37152539
- Shen J et al. TLR9 regulates NLRP3 inflammasome activation via the NF-kB signaling pathway in diabetic nephropathy. Diabetol Metab Syndr 14:26 (2022). PubMed: 35120573
- Lai RH et al. Neurotropic EV71 causes encephalitis by engaging intracellular TLR9 to elicit neurotoxic IL12-p40-iNOS signaling. Cell Death Dis 13:328 (2022). PubMed: 35399111