重组Anti-TLR7抗体[EPR2088(2)] (ab124928)
![Western blot - Anti-TLR7 antibody [EPR2088(2)] (ab124928)](https://www.abcam.cn/ps/products/124/ab124928/Images/ab124928-506561-anti-tlr7-antibody-epr2088-2-western-blot-ramos-daudi-human.png "Western blot - Anti-TLR7 antibody [EPR2088(2)] (ab124928)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR2088(2)] to TLR7
- Suitable for: WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-TLR7抗体[EPR2088(2)]
参阅全部 TLR7 一抗 -
描述
兔单克隆抗体[EPR2088(2)] to TLR7 -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-Pmore details
不适用于: Flow Cyt or ICC/IF -
种属反应性
与反应: Human -
免疫原
Synthetic peptide within Human TLR7 aa 1000 to the C-terminus (C terminal). The exact sequence is proprietary.
Database link: Q9NYK1 -
阳性对照
- WB: Raji, Daudi and Ramos cell lysates. IHC-P: Human tonsil tissue.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
解离常数（KD）
KD = 2.90 x 10 -11 M Learn more about KD -
存储溶液
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA, 59% PBS -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR2088(2) -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab124928于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (2) |
1/1000. Detects a band of approximately 140 kDa (predicted molecular weight: 121 kDa).
For unpurified use at 1/300. To achieve desired WB results, do not to boil samples after lysis. |
| IHC-P | (3) |
1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
See IHC antigen retrieval protocols. For unpurified use at 1/30. |
| 说明 |
|---|
|
WB
1/1000. Detects a band of approximately 140 kDa (predicted molecular weight: 121 kDa). For unpurified use at 1/300. To achieve desired WB results, do not to boil samples after lysis. |
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IHC-P
1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. See IHC antigen retrieval protocols. For unpurified use at 1/30. |
靶标
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功能
Key component of innate and adaptive immunity. TLRs (Toll-like receptors) control host immune response against pathogens through recognition of molecular patterns specific of microorganisms. TLR7 is a nucleotide-sensing TLR which is activated by single-stranded RNA. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. -
组织特异性
Detected in brain, placenta, spleen, stomach, small intestine, lung and in plasmacytoid pre-dendritic cells. -
序列相似性
Belongs to the Toll-like receptor family.
Contains 27 LRR (leucine-rich) repeats.
Contains 1 TIR domain. -
细胞定位
Endoplasmic reticulum membrane. Endosome. Lysosome. Cytoplasmic vesicle > phagosome. Relocalizes from endoplasmic reticulum to endosome and lysosome upon stimulation with agonist. - Information by UniProt
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数据库链接
- Entrez Gene: 51284 Human
- Omim: 300365 Human
- SwissProt: Q9NYK1 Human
- Unigene: 659215 Human
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别名
- PRO285 antibody
- TLR 7 antibody
- Tlr7 antibody
see all
图片
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Western blot - Anti-TLR7 antibody [EPR2088(2)] (ab124928)All lanes : Anti-TLR7 antibody [EPR2088(2)] (ab124928) at 1/1000 dilution
Lane 1 : Ramos (Human Burkitt's lymphoma B lymphocyte) whole cell lysate (boiled)
Lane 2 : Ramos (Human Burkitt's lymphoma B lymphocyte) whole cell lysate (unboiled)
Lane 3 : Daudi (Human Burkitt's lymphoma lymphoblast) whole cell lysate (boiled)
Lane 4 : Daudi (Human Burkitt's lymphoma lymphoblast) whole cell lysate (unboiled)
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 121 kDa
Observed band size: 140 kDa why is the actual band size different from the predicted?
Exposure time: 20 secondsBlocking and diluting buffer and concentration: 5% NFDM /TBST.
We recommend not boiling the samples after lysis to get desired WB results.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TLR7 antibody [EPR2088(2)] (ab124928)](https://www.abcam.cn/ps/products/124/ab124928/Images/ab124928-138948-anti-tlr7-antibody-epr20882-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TLR7 antibody [EPR2088(2)] (ab124928)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue labelling TLR7 with unpurified ab124928 at 1/100.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TLR7 antibody [EPR2088(2)] (ab124928)](https://www.abcam.cn/ps/products/124/ab124928/Images/ab124928-237993-anti-tlr7-antibody-epr20882-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TLR7 antibody [EPR2088(2)] (ab124928)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue labelling TLR7 with purified ab124928 at 1/100. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. A prediluted HRP-polymer conjugated anti-rabbit IgG was used as the secondary antibody. Counterstained with hematoxylin.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TLR7 antibody [EPR2088(2)] (ab124928)](https://www.abcam.cn/ps/products/124/ab124928/Images/ab124928-237994-anti-tlr7-antibody-epr20882-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TLR7 antibody [EPR2088(2)] (ab124928)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue labelling TLR7 with unpurified ab124928 at 1/100. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. A prediluted HRP-polymer conjugated anti-rabbit IgG was used as the secondary antibody. Counterstained with hematoxylin.
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![OI-RD Scanning - Anti-TLR7 antibody [EPR2088(2)] (ab124928)](https://www.abcam.cn/ps/products/124/ab124928/Images/ab124928-138947-anti-tlr7-antibody-epr20882.jpg)
OI-RD Scanning - Anti-TLR7 antibody [EPR2088(2)] (ab124928) -
![Anti-TLR7 antibody [EPR2088(2)] (ab124928)](https://www.abcam.cn/ps/products/124/ab124928/Images/ab124928-8-benefits-of-recombinant-antibodies.png)
Anti-TLR7 antibody [EPR2088(2)] (ab124928)
数据表及文件
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SDS download
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Datasheet download
文献 (14)
ab124928 被引用在 14 文献中.
- Zhang Y et al. Triptolide in the treatment of systemic lupus erythematosus - regulatory effects on miR-146a in B cell TLR7 signaling pathway in mice. Front Pharmacol 13:952775 (2022). PubMed: 36210830
- Bouvet M et al. Multiple Viral microRNAs Regulate Interferon Release and Signaling Early during Infection with Epstein-Barr Virus. mBio 12:N/A (2021). PubMed: 33785626
- Proskocil BJ et al. TLR7 is expressed by support cells, but not sensory neurons, in ganglia. J Neuroinflammation 18:209 (2021). PubMed: 34530852
- Zheng N et al. TLR7 in B cells promotes renal inflammation and Gd-IgA1 synthesis in IgA nephropathy. JCI Insight 5:N/A (2020). PubMed: 32699192
- Azar P et al. TLR7 dosage polymorphism shapes interferogenesis and HIV-1 acute viremia in women. JCI Insight 5:N/A (2020). WB . PubMed: 32554924