Anti-TLR7抗体[4F4] (ab28048)
![Western blot - Anti-TLR7 antibody [4F4] (ab28048)](https://www.abcam.cn/ps/products/28/ab28048/Images/ab28048-38952-anti-tlr7-antibody-4f4-western-blot.jpg "Western blot - Anti-TLR7 antibody [4F4] (ab28048)")
Key features and details
- Mouse monoclonal [4F4] to TLR7
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG1
概述
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产品名称
Anti-TLR7抗体[4F4]
参阅全部 TLR7 一抗 -
描述
小鼠单克隆抗体[4F4] to TLR7 -
宿主
Mouse -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Human -
免疫原
Recombinant fragment corresponding to Human TLR7 aa 450-550.
Database link: Q9NYK1 -
阳性对照
- Ramos cell lysates
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常规说明
This product was changed from ascites to tissue culture supernatant on 28/02/19. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 10% Glycerol -
Concentration information loading... -
纯度
Protein G purified -
克隆
单克隆 -
克隆编号
4F4 -
骨髓瘤
Sp2/0 -
同种型
IgG1 -
轻链类型
kappa -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab28048于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/500 - 1/2000. Predicted molecular weight: 121 kDa.
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| 说明 |
|---|
|
WB
1/500 - 1/2000. Predicted molecular weight: 121 kDa. |
靶标
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功能
Key component of innate and adaptive immunity. TLRs (Toll-like receptors) control host immune response against pathogens through recognition of molecular patterns specific of microorganisms. TLR7 is a nucleotide-sensing TLR which is activated by single-stranded RNA. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. -
组织特异性
Detected in brain, placenta, spleen, stomach, small intestine, lung and in plasmacytoid pre-dendritic cells. -
序列相似性
Belongs to the Toll-like receptor family.
Contains 27 LRR (leucine-rich) repeats.
Contains 1 TIR domain. -
细胞定位
Endoplasmic reticulum membrane. Endosome. Lysosome. Cytoplasmic vesicle > phagosome. Relocalizes from endoplasmic reticulum to endosome and lysosome upon stimulation with agonist. - Information by UniProt
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数据库链接
- Entrez Gene: 51284 Human
- Omim: 300365 Human
- SwissProt: Q9NYK1 Human
- Unigene: 659215 Human
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别名
- PRO285 antibody
- TLR 7 antibody
- Tlr7 antibody
see all
图片
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Western blot - Anti-TLR7 antibody [4F4] (ab28048)Anti-TLR7 antibody [4F4] (ab28048) at 1/1000 dilution + 80 µg Ramos cell lysate
Secondary
goat anti mouse secondary antibody conjugated to HRP
Developed using the ECL technique.
Predicted band size: 121 kDa
Observed band size: 121 kDaThis image was generated using the ascites version of the product.
数据表及文件
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SDS download
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Datasheet download
文献 (3)
ab28048 被引用在 3 文献中.
- Makni-Maalej K et al. The TLR7/8 agonist CL097 primes N-formyl-methionyl-leucyl-phenylalanine-stimulated NADPH oxidase activation in human neutrophils: critical role of p47phox phosphorylation and the proline isomerase Pin1. J Immunol 189:4657-65 (2012). WB . PubMed: 23002436
- Wang K et al. Correlation of TLR1-10 expression in peripheral blood mononuclear cells with chronic hepatitis B and chronic hepatitis B-related liver failure. Hum Immunol 71:950-6 (2010). WB ; Human . PubMed: 20667457
- Gregory SM et al. Toll-like receptor signaling controls reactivation of KSHV from latency. Proc Natl Acad Sci U S A 106:11725-30 (2009). WB ; Human . PubMed: 19564611