Anti-TLR5抗体(ab62460)
Key features and details
- Rabbit polyclonal to TLR5
- Suitable for: WB, IHC-P
- Reacts with: Rat
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术，可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-TLR5抗体
参阅全部 TLR5 一抗 -
描述
兔多克隆抗体to TLR5 -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-Pmore details -
种属反应性
与反应: Rat -
免疫原
A 16 amino acid synthetic peptide near the center of human TLR5
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阳性对照
- Rat brain lysate, brain tissue
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. -
存储溶液
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab62460于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (1) |
Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 97 kDa (predicted molecular weight: 97 kDa).
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| IHC-P | (3) |
Use a concentration of 10 µg/ml.
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| 说明 |
|---|
|
WB
Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 97 kDa (predicted molecular weight: 97 kDa). |
|
IHC-P
Use a concentration of 10 µg/ml. |
靶标
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功能
Participates in the innate immune response to microbial agents. Mediates detection of bacterial flagellins. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. -
组织特异性
Highly expressed in ovary and in peripheral blood leukocytes, especially in monocytes, less in CD11c+ immature dendritic cells. Also detected in prostate and testis. -
疾病相关
Systemic lupus erythematosus 1 -
序列相似性
Belongs to the Toll-like receptor family.
Contains 22 LRR (leucine-rich) repeats.
Contains 1 LRRCT domain.
Contains 1 TIR domain. -
翻译后修饰
Phosphorylated at Ser-805 by PKD/PRKD1; phosphorylation induces the production of inflammatory cytokines.
Phosphorylated at Tyr-798 upon flagellin binding; required for signaling. -
细胞定位
Membrane. - Information by UniProt
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数据库链接
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别名
- FLJ10052 antibody
- MGC126430 antibody
- MGC126431 antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TLR5 antibody (ab62460)Immunohistochemistry of TLR5 on paraffin embedded rat brain tissue section with ab62460 at 10 µg/ml.
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Western blot - Anti-TLR5 antibody (ab62460)Lane 1 : Anti-TLR5 antibody (ab62460) at 0.5 µg/ml
Lane 2 : Anti-TLR5 antibody (ab62460) at 1 µg/ml
Lane 3 : Anti-TLR5 antibody (ab62460) at 2 µg/ml
All lanes : Rat brain cell lysate
Lysates/proteins at 15 µg per lane.
Predicted band size: 97 kDa
Observed band size: 97 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TLR5 antibody (ab62460)Image courtesy of Dr Ilka Werner-Martini by Abreviewab62460 staining TLR5 in human pancreatic tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue was fixed with formaldehyde and a heat mediated antigen retrieval step was performed. Samples were then incubated with the primary antibody at a 1/50 dilution for 14 hours at 4°C. An undiluted HRP-conjugated goat polyclonal was used as secondary antibody.
数据表及文件
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SDS download
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Datasheet download
文献 (12)
ab62460 被引用在 12 文献中.
- Tian X et al. Expression of TLR2 and TLR5 in distal ileum of mice with obstructive jaundice and their role in intestinal mucosal injury. Arch Med Sci 18:237-250 (2022). PubMed: 35154543
- Rao K et al. Shenling Baizhu Powder Alleviates TNBS-Induced Colitis in Rats by Improving Intestinal Epithelial Permeability and Inhibiting Inflammation Through the TLR5/MyD88/NF-κB Pathway. Front Pharmacol 13:883918 (2022). PubMed: 35571126
- Min HK et al. Relationship between toll-like receptor expression in the distal facial nerve and facial nerve recovery after injury. Int J Immunopathol Pharmacol 36:3946320221090007 (2022). PubMed: 35585682
- Yu JI et al. Pneumococcal Δpep27 Immunization Attenuates TLRs and NLRP3 Expression and Relieves Murine Ovalbumin-Induced Allergic Rhinitis. J Microbiol Biotechnol 32:709-717 (2022). PubMed: 35484967
- Zhang J et al. Expression and function of Toll-like receptors in peripheral blood mononuclear cells in patients with ankylosing spondylitis. Mol Med Rep 20:3565-3572 (2019). PubMed: 31485664