Anti-TLR5抗体(ab37071)
Key features and details
- Rabbit polyclonal to TLR5
- Suitable for: ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-TLR5抗体
参阅全部 TLR5 一抗 -
描述
兔多克隆抗体to TLR5 -
宿主
Rabbit -
经测试应用
适用于: ICC/IF, WBmore details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide corresponding to 15 amino acids near the carboxy terminus or human TLR5.
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阳性对照
- THP 1 cell lysate
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. -
存储溶液
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading...
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纯度
Protein A purified -
纯化说明
Purified by affinity chromatography -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Immunizing Peptide (Blocking)
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab37071于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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ICC/IF |
Use a concentration of 10 µg/ml.
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WB |
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 97 kDa.Can be blocked with Active human TLR5 peptide (ab40018).
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说明 |
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ICC/IF
Use a concentration of 10 µg/ml. |
WB
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 97 kDa.Can be blocked with Active human TLR5 peptide (ab40018). |
靶标
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功能
Participates in the innate immune response to microbial agents. Mediates detection of bacterial flagellins. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. -
组织特异性
Highly expressed in ovary and in peripheral blood leukocytes, especially in monocytes, less in CD11c+ immature dendritic cells. Also detected in prostate and testis. -
疾病相关
Systemic lupus erythematosus 1 -
序列相似性
Belongs to the Toll-like receptor family.
Contains 22 LRR (leucine-rich) repeats.
Contains 1 LRRCT domain.
Contains 1 TIR domain. -
翻译后修饰
Phosphorylated at Ser-805 by PKD/PRKD1; phosphorylation induces the production of inflammatory cytokines.
Phosphorylated at Tyr-798 upon flagellin binding; required for signaling. -
细胞定位
Membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 7100 Human
- Omim: 603031 Human
- SwissProt: O60602 Human
- Unigene: 604542 Human
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别名
- FLJ10052 antibody
- MGC126430 antibody
- MGC126431 antibody
see all
图片
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Lane 1 : Anti-TLR5 antibody (ab37071) at 0.5 µg/ml
Lane 2 : Anti-TLR5 antibody (ab37071) at 1 µg/ml
Lane 3 : Anti-TLR5 antibody (ab37071) at 2 µg/ml
All lanes : THP 1 cell lysate
Lysates/proteins at 15 µg per lane.
Predicted band size: 97 kDa
Observed band size: 97 kDa
Additional bands at: 65 kDa. We are unsure as to the identity of these extra bands. -
ab37071 at 10µg/ml staining TLR5 in THP-1 cells by ICC/IF
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Immunofluorescence of TLR5 in THP1 cells using ab37071 at 10 ug/ml.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab37071 被引用在 1 文献中.
- Harbuzariu A et al. Neuregulin-1/ErbB4 signaling modulates Plasmodium falciparum HRP2-induced damage to brain cortical organoids. iScience 25:104407 (2022). PubMed: 35663028