Anti-TLR4抗体(ab13556)
Key features and details
- Rabbit polyclonal to TLR4
- Suitable for: WB, Flow Cyt
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-TLR4抗体
参阅全部 TLR4 一抗 -
描述
兔多克隆抗体to TLR4 -
宿主
Rabbit -
特异性
TLR4 expression levels and cleavage or degradation products can vary between different cell and tissue samples. Customers have observed this variability in WB band size and our laboratory has confirmed this variability as well observing lower molecular weight cleavage and degradation products and in some samples a lack of the full length TLR4 band. The TLR4 cleavage and degradation products and potential lack of full length TLR4 are well documented in the literature, including PMID 16885150 and 22927440. We recommend running a positive control human intestine tissue lysate. We have obtained both positive and negative feedback from researchers using this antibody with rat samples (see Abreviews). Due to the inconsistency, we have removed rat as a guaranteed species and welcome any further feedback from researchers using this antibody. -
经测试应用
适用于: WB, Flow Cytmore details
不适用于: IHC-Fr or IHC-P -
种属反应性
与反应: Human -
免疫原
Synthetic peptide corresponding to Human TLR4 aa 420-435.
Sequence:GLEQLEHLDFQ HSNLK
Database link: O00206 -
阳性对照
- WB: HEK-293T overexpressing TLR4 (unboiled) cell lysate
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.09% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading... -
纯度
Protein G purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab13556于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (5) |
1/1000. Predicted molecular weight: 75-80 kDa.
Block in 5% Skim Milk in 1X TBST. Other potential band(s): ~130kDa (TLR4+MD-2). We suggest use TLR4 overexpression 293T lysate (whole cell) (ab94063) as positive control. |
| Flow Cyt | (3) |
Use at an assay dependent concentration.
ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody. |
| 说明 |
|---|
|
WB
1/1000. Predicted molecular weight: 75-80 kDa. Block in 5% Skim Milk in 1X TBST. Other potential band(s): ~130kDa (TLR4+MD-2). We suggest use TLR4 overexpression 293T lysate (whole cell) (ab94063) as positive control. |
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Flow Cyt
Use at an assay dependent concentration. ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody. |
靶标
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功能
Cooperates with LY96 and CD14 to mediate the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MYD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Also involved in LPS-independent inflammatory responses triggered by Ni(2+). These responses require non-conserved histidines and are, therefore, species-specific. -
组织特异性
Highly expressed in placenta, spleen and peripheral blood leukocytes. Detected in monocytes, macrophages, dendritic cells and several types of T-cells. -
疾病相关
Genetic variation in TLR4 is associated with age-related macular degeneration type 10 (ARMD10) [MIM:611488]. ARMD is a multifactorial eye disease and the most common cause of irreversible vision loss in the developed world. In most patients, the disease is manifest as ophthalmoscopically visible yellowish accumulations of protein and lipid that lie beneath the retinal pigment epithelium and within an elastin-containing structure known as Bruch membrane. -
序列相似性
Belongs to the Toll-like receptor family.
Contains 18 LRR (leucine-rich) repeats.
Contains 1 LRRCT domain.
Contains 1 TIR domain. -
结构域
The TIR domain mediates interaction with NOX4. -
翻译后修饰
N-glycosylated. Glycosylation of Asn-526 and Asn-575 seems to be necessary for the expression of TLR4 on the cell surface and the LPS-response. Likewise, mutants lacking two or more of the other N-glycosylation sites were deficient in interaction with LPS. -
细胞定位
Membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 7099 Human
- Omim: 603030 Human
- SwissProt: O00206 Human
- Unigene: 174312 Human
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别名
- ARMD10 antibody
- CD284 antibody
- CD284 antigen antibody
see all
图片
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Western blot - Anti-TLR4 antibody (ab13556)All lanes : Anti-TLR4 antibody (ab13556) at 1/1000 dilution
Lane 1 : HEK-293T overexpressing TLR4 (unboiled) cell lysate
Lane 2 : HEK-293T untransfected (unboiled) cell lysate
Lysates/proteins at 20 µg per lane.
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 75-80 kDa
Observed band size: 70 kDa why is the actual band size different from the predicted?Western blot: Anti-TLR4 antibody (ab13556) staining at 1/1000 dilution, shown in green; Mouse anti-GAPDH antibody [6C5] (ab8245) loading control staining at 1/20000 dilution, shown in magenta. In Western blot, ab13556 was shown to bind specifically to TLR4. First, unboiled samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in fluorescent western blot (TBS-based) blocking solution before incubation with primary antibodies overnight at 4°C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.
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Flow Cytometry - Anti-TLR4 antibody (ab13556)Flow cytometry analysis of THP-1 (Human monocytic leukemia cell line). Cells were fixed with 2% formaldehyde for 10 minutes at room temperature. ab13556 was used at 2 µg/106 cells for 60 minutes at 37°C (green). Goat Anti- Rabbit Dylight 488 was used as a secondary antibody at 1/200 dilution for 40 minutes at 37°C. Isotype control was Rabbit IgG under the same conditions (blue).
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (335)
ab13556 被引用在 335 文献中.
- Yan Z et al. TREM1 is involved in the mechanism between asthma and lung cancer by regulating the Toll‑like receptor signaling pathway. Oncol Lett 27:16 (2024). PubMed: 38028174
- Li H et al. Apigenin attenuates inflammatory response in allergic rhinitis mice by inhibiting the TLR4/MyD88/NF-κB signaling pathway. Environ Toxicol 38:253-265 (2023). PubMed: 36350155
- Scheid S et al. Argon preconditioning protects neuronal cells with a Toll-like receptor-mediated effect. Neural Regen Res 18:1371-1377 (2023). PubMed: 36453425
- Huang J et al. Effect of curcumin on regulatory B cells in chronic colitis mice involving TLR/MyD88 signaling pathway. Phytother Res 37:731-742 (2023). PubMed: 36196887
- Guan L et al. NFE2L2 Mutations Enhance Radioresistance in Head and Neck Cancer by Modulating Intratumoral Myeloid Cells. Cancer Res 83:861-874 (2023). PubMed: 36652552