Anti-TLR4抗体[76B357.1] (ab22048)
Key features and details
- Mouse monoclonal [76B357.1] to TLR4
- Suitable for: Flow Cyt (Intra), ICC/IF, IHC-P, ICC, IHC-Fr, ELISA
- Reacts with: Mouse, Rat, Human, Pig
- Isotype: IgG2b
概述
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产品名称
Anti-TLR4抗体[76B357.1]
参阅全部 TLR4 一抗 -
描述
小鼠单克隆抗体[76B357.1] to TLR4 -
宿主
Mouse -
特异性
TLR4 expression levels and cleavage or degradation products can vary between different cell and tissue samples. Customers have observed this variability in WB band size and our laboratory has confirmed this variability as well observing lower molecular weight cleavage and degradation products and in some samples a lack of the full length TLR4 band. The TLR4 cleavage and degradation products and potential lack of full length TLR4 are well documented in the literature, including PMID 16885150 and 22927440. We recommend running a positive control human intestine tissue lysate. -
经测试应用
适用于: Flow Cyt (Intra), ICC/IF, IHC-P, ICC, IHC-Fr, ELISAmore details -
种属反应性
与反应: Mouse, Rat, Human, Pig
预测可用于: Sheep, Horse, Cow, Cat, Chimpanzee -
免疫原
Synthetic peptide corresponding to Human TLR4 aa 100-200 conjugated to Keyhole Limpet Haemocyanin (KLH).
Database link: O00206 -
阳性对照
- Human, Mouse and Rat small intestine for Western Blot or THP1 cells for FACS analysis. Flow Cyt (Intra): Jurkat cells.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.05% Sodium azide
Constituents: 99% PBS, 0.05% BSA -
Concentration information loading...
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纯度
Protein G purified -
克隆
单克隆 -
克隆编号
76B357.1 -
同种型
IgG2b -
轻链类型
kappa -
研究领域
相关产品
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Buffer
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab22048于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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Flow Cyt (Intra) |
Use 1µg for 106 cells.
Methanol or paraformaldehyde fixed cells. ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
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ICC/IF | (5) |
Use at an assay dependent concentration.
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IHC-P | (5) |
Use at an assay dependent concentration.
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ICC | (3) |
Use at an assay dependent concentration.
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IHC-Fr | (2) |
Use at an assay dependent concentration.
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ELISA |
Use at an assay dependent concentration. PubMed: 24952384
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说明 |
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Flow Cyt (Intra)
Use 1µg for 106 cells. Methanol or paraformaldehyde fixed cells. ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
ICC/IF
Use at an assay dependent concentration. |
IHC-P
Use at an assay dependent concentration. |
ICC
Use at an assay dependent concentration. |
IHC-Fr
Use at an assay dependent concentration. |
ELISA
Use at an assay dependent concentration. PubMed: 24952384 |
靶标
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功能
Cooperates with LY96 and CD14 to mediate the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MYD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Also involved in LPS-independent inflammatory responses triggered by Ni(2+). These responses require non-conserved histidines and are, therefore, species-specific. -
组织特异性
Highly expressed in placenta, spleen and peripheral blood leukocytes. Detected in monocytes, macrophages, dendritic cells and several types of T-cells. -
疾病相关
Genetic variation in TLR4 is associated with age-related macular degeneration type 10 (ARMD10) [MIM:611488]. ARMD is a multifactorial eye disease and the most common cause of irreversible vision loss in the developed world. In most patients, the disease is manifest as ophthalmoscopically visible yellowish accumulations of protein and lipid that lie beneath the retinal pigment epithelium and within an elastin-containing structure known as Bruch membrane. -
序列相似性
Belongs to the Toll-like receptor family.
Contains 18 LRR (leucine-rich) repeats.
Contains 1 LRRCT domain.
Contains 1 TIR domain. -
结构域
The TIR domain mediates interaction with NOX4. -
翻译后修饰
N-glycosylated. Glycosylation of Asn-526 and Asn-575 seems to be necessary for the expression of TLR4 on the cell surface and the LPS-response. Likewise, mutants lacking two or more of the other N-glycosylation sites were deficient in interaction with LPS. -
细胞定位
Membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 281536 Cow
- Entrez Gene: 7099 Human
- Entrez Gene: 21898 Mouse
- Entrez Gene: 399541 Pig
- Entrez Gene: 29260 Rat
- Omim: 603030 Human
- SwissProt: Q9GL65 Cow
- SwissProt: Q9MYW3 Horse
see all -
别名
- ARMD10 antibody
- CD284 antibody
- CD284 antigen antibody
see all
图片
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Immunohistochemical analysis of paraffin-embedded Human skin tissue labeling TLR4 with ab22048 at 5 ug/ml. The section was blocked using blocking solution (1% BSA in PBS) for 1 hour at room temperature and incubated with ab22048 overnight at 4°C after removing blocking solution.
Antigen retrieval: Bring slides to a boil in 10 mM sodium citrate buffer (pH 6.0) then maintain at a sub-boiling temperature for 10 minutes. Cool slides on bench-top for 30 minutes (keep slides in the sodium citrate buffer all the time).
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ab22048 staining TLR4 in Human stomach tissue sections by Immunohistochemistry (IHC-P - formaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 5% serum for 1 hour; antigen retrieval was by heat mediation in citrate buffer (10mM, pH 6) (ab64236). Samples were incubated with primary antibody (1/100) for 1 hour at 23°C. An undiluted HRP-conjugated goat anti-mouse IgG polyclonal was used as the secondary antibody.
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Overlay histogram showing Jurkat cells (ab7899) stained with ab22048 (red line). The cells were fixed with methanol (5 min) and incubated in 1x PBS / 10% normal goat serum (ab7481) / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab22048, 1µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in Jurkat cells fixed with 4% paraformaldehyde (10 min) used under the same conditions.
Please note that Abcam do not have data for use of this antibody on non-fixed cells. We welcome any customer feedback. -
Representative examples of immunohistochemical staining of TLR4 in colorectal carcinoma tissues (original magnification 100×).
Paraffin-embedded human normal colorectal tissue (A) and colorectal carcinoma tissue (D) stained for TLR4 with ab22048 in immunohistochemical analysis. Counter stained with hematoxylin.
No staining is observed in normal colorectal tissue (A).
(From Figure 4A and 4D of Xu et al)
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ab22048 (5 ug/ml) staining TLR4 in Human skin tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Membrane-cytoplasmic immunopositivity of TLR4 was primarily observed in the pigmented basel cells and the adjacent keratinocytes in the epidermal layer.
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ab22048 at 5 µg/ml staining TLR4 in Human colon tissue sections by Immunohistochemistry (Formalin/ PFA-fixed paraffin-embedded tissue sections). Left hand image shows staining with an isotype control antibody whilst right one shows staining with ab22048.
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ab22048 staining TLR4 in Rat's salivary gland tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). ab22048 at a dilution of 1:100 generated a membrane-cytoplasmic staining in the tissue with stronger signal in ductal epithelial cells.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (261)
ab22048 被引用在 261 文献中.
- Zheng Z et al. E. coli JM83 damages the mucosal barrier in Ednrb knockout mice to promote the development of Hirschsprung‑associated enterocolitis via activation of TLR4/p‑p38/NF‑κB signaling. Mol Med Rep 25:N/A (2022). PubMed: 35302172
- Li S et al. The Protective Effects of Ginseng Polysaccharides and Their Effective Subfraction against Dextran Sodium Sulfate-Induced Colitis. Foods 11:N/A (2022). PubMed: 35327312
- Liu L et al. The transmembrane adapter SCIMP recruits tyrosine kinase Syk to phosphorylate Toll-like receptors to mediate selective inflammatory outputs. J Biol Chem 298:101857 (2022). PubMed: 35337798
- Zheng H et al. Cordyceps militaris Modulates Intestinal Barrier Function and Gut Microbiota in a Pig Model. Front Microbiol 13:810230 (2022). PubMed: 35369439
- Qu P et al. Application Value of Serum Hcy, TLR4, and CRP in the Diagnosis of Cerebral Small Vessel Disease. Evid Based Complement Alternat Med 2022:4025965 (2022). PubMed: 35502170