Anti-TLR3抗体[TLR3.7] (ab12085)

I heard back from our lab that the most recent lot of ab12085 had an endotoxin level of 0.02EU/ml. The ab17264 has not been tested for endotoxin.

I can confirm that IgG1 has a mass of ca 150kDa.

https://www.abcam.com/index.html?pageconfig=resource&rid=11258&pid=10996

This should not change very much between different mouse IgG1 (unconjugated) antibodies.

ab12085 has not been specifically tested for its molecular weight and therefore we assume that it is in line with being a mouse IgG1 and therefore ca 150kDa.

If you are looking at a gel of this product, I would like to note that it also contains BSA.

Thank you for contacting us.


The biological activity of the antibody has been described in rthe following reference from datasheet:

Matsumoto, M et al; Establishment of a monoclonal antibody against human Toll-like receptor 3 that blocks double-stranded RNA-mediated signalling. Biochem Biophys Res Commun 2002, 293: 1364


Monoclonal antibody TLR3.7 inhibits dsRNA-induces IFN-beta production. 7.5*10E4 MRC5 cells were pre-treated with 10-20 µg/ml for 1-24h at 37°C.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Thank you for contacting us.


ab12085 can be used to inhibit the function of TLR3. The following publication describe the use of this clone in an inhibitory assay:

Matsumoto, M et al; Establishment of a monoclonal antibody against human Toll-like receptor 3 that blocks double-stranded RNA-mediated signalling. Biochem Biophys Res Commun 2002, 293: 1364.

PMID:12054664

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Thank you for contacting us regarding ab65355, cAMP Direct Immunoassay kit. As requested I have created a credit note for the original purchase. Your credit note ID is . I am sorry that this antibody did not perform as stated on the datasheet. I have asked our accounting department to issue a credit note for you, which can be redeemed against the invoice of a future order by passing it on to your purchasing department. To avoid confusion, please ensure your accounts department is aware of how the credit note is being used. If you have questions on how to use the credit note, please contact our accounting department. If payment has already been made on the original order and you wish to receive a refund, please ask your purchasing department to contact our accounting department so that we may gather the correct information needed for the refund. To avoid confusion, please ensure your accounts department is aware of how the credit note is being used. Our accounting department can be contacted by email at us.credits@abcam.com or by telephone using the information at the Contact Us link in the top right corner of our website. Please refer to the credit note ID in any correspondence with our accounting department. The credit note ID is for your reference only and does not automatically guarantee the credit. I hope this experience will not prevent you from purchasing other products from us in the future. Our Scientific Support team is always at your service, should you require further expert advice.

Thank you for contacting us. Unfortunately Anti-TLR3 antibody [TLR3.7] (ab12085) has not been tested for neutralising activity in rat. More information on the antibodies functional activity can be found from the following reference: Matsumoto M et al. Establishment of a monoclonal antibody against human Toll-like receptor 3 that blocks double-stranded RNA-mediated signaling. Biochem Biophys Res Commun 293:1364-9 (2002). PubMed: 12054664 The antibody is known to bind the extracellular domain of TRL3. As this antibody is directed against human TRL3 the homology of this region with that of Rat needs to be taken into consideration when predicting if the antibody is likely to bind the rat protein. Through sequence comparison these two regions share 78% homology. We usually predict homologies of 85% and above to react. I can therefore not recommend using this antibody with rat samples. We only have two antibodies, ab62566 and ab117353 which have been used with rat protein but neither has been tested for their neutralising behaviour. I'm sorry I could not be of more help. If you need any further information please do not hesitate to ask.

Thank you for contacting us. The following reference describes the development of the TLR3.7 clone and its use in an inhibition assay: Matsumoto, M et al; Establishment of a monoclonal antibody against human Toll-like receptor 3 that blocks double-stranded RNA-mediated signalling. Biochem Biophys Res Commun 2002, 293: 1364. PMID:12054664 I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

以外と製造元の返事が早かったです。 この回答は電話で申し上げにくいかと思いますので、メールで送った方がよいではないかと思います。 ***製造元よりの情報*** 中和（inhibition）の詳細は下記の文献に載ってありますので、ご参考いただけますようお願いいたします。 1) Matsumoto, M et al; Establishment of a monoclonal antibody against human Toll-like receptor 3 that blocks double-stranded RNA-mediated signalling. Biochem Biophys Res Commun 2002, 293: 1364 Antibody blocking and IFN-b assays. MRC-5 cells in 24-well plates (7.5*104) were pre-treated with 20 lg/ml of anti-TLR2 mAb (TLR2.45), or anti-TLR3 mAb (TLR3.7) for 1 h at 37 °C, then stimulated with polymyxin B-treated poly(I):poly(C) (5, 10 lg/ml) for 24 h. The concentrations of IFN-b in the culture supernatants were measured by ELISA (TFB Inc.). 2) Matsumoto, M et al; Subcellular localization of Toll-like receptor 3 in human dendritic cells. J Immunol 2003, 171: 3154 そして、マウスへの使用に関しては実際テストされたことがございません。万が一、実験で期待通りの結果を得られなかった場合、保証の提供ができかねます。しかし、そうはいっても、必ずしも結果が得られない訳でもございません。 ***ここまで*** マウスにテストされていないので、Abreview　100% ディスカウントを案内してもよいと思います。もしお客様が興味がありましたら、私からご案内します。その際、メールアドレスさえをご連絡いただけると大変助かります。

Thank you for contacting us for technical support regarding ab12085. This antibody has not been tested against other species than human and I would recommend running in parallel with your samples the positive controls of human placenta or pancreas lysate. There is a 80% homology between the human form and the mouse form of TLR3, so although this is not low as the antibody is a monoclonal antibody it will only recognise one epitope and if this epitope is not homologous in the human and rat and mouse the antibody will fail to cross react with your samples. I am in touch with the laboratory to try to find out other experimental details which should help, such as the lysis buffer to make sure the protein is well extracted, I'm sorry for the delay and appreciate your patience,