Anti-TLR3抗体(ab62566)
Key features and details
- Rabbit polyclonal to TLR3
- Suitable for: IHC-P, ICC, WB
- Reacts with: Mouse, Human
- Isotype: IgG
概述
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产品名称
Anti-TLR3抗体
参阅全部 TLR3 一抗 -
描述
兔多克隆抗体to TLR3 -
宿主
Rabbit -
经测试应用
适用于: IHC-P, ICC, WBmore details -
种属反应性
与反应: Mouse, Human -
免疫原
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阳性对照
- IHC-P: Human and mouse spleen tissue. WB: Daudi cell lysate and mouse speen tissue. ICC: Human spleen tissue.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at +4°C. -
存储溶液
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading... -
纯度
Affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab62566于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P | (2) |
Use a concentration of 2 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Also see abreview 35252. |
| ICC |
Use a concentration of 1 µg/ml.
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| WB | (6) |
Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 104 kDa (predicted molecular weight: 104 kDa).
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| 说明 |
|---|
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IHC-P
Use a concentration of 2 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Also see abreview 35252. |
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ICC
Use a concentration of 1 µg/ml. |
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WB
Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 104 kDa (predicted molecular weight: 104 kDa). |
靶标
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功能
Key component of innate and adaptive immunity. TLRs (Toll-like receptors) control host immune response against pathogens through recognition of molecular patterns specific of microorganisms. TLR3 is a nucleotide-sensing TLR which is activated by double-stranded RNA, a sign of viral infection. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. -
组织特异性
Expressed at high level in placenta and pancreas. Also detected in CD11c+ immature dendritic cells. Only expressed in dendritic cells and not in other leukocytes, including monocyte precursors. TLR3 is the TLR that is expressed most strongly in the brain, especially in astrocytes, glia, and neurons. -
疾病相关
Defects in TLR3 are associated with herpes simplex encephalitis type 2 (HSE2) [MIM:613002]. HSE is a rare complication of human herpesvirus 1 (HHV-1) infection, occurring in only a small minority of HHV-1 infected individuals. HSE is characterized by hemorrhagic necrosis of parts of the temporal and frontal lobes. Onset is over several days and involves fever, headache, seizures, stupor, and often coma, frequently with a fatal outcome. Note=TLR3 mutations predispose otherwise healthy individuals to isolated herpes simplex encephalitis through a mechanism that involves impaired IFNs production and reduced immune defense against viral infection in the central nervous system. -
序列相似性
Belongs to the Toll-like receptor family.
Contains 22 LRR (leucine-rich) repeats.
Contains 1 LRRCT domain.
Contains 1 LRRNT domain.
Contains 1 TIR domain. -
结构域
ds-RNA binding is mediated by LRR 1 to 3, and LRR 17 to 18. -
翻译后修饰
Heavily N-glycosylated, except on that part of the surface of the ectodomain that is involved in ligand binding. -
细胞定位
Endoplasmic reticulum membrane. Endosome membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 7098 Human
- Entrez Gene: 142980 Mouse
- Omim: 603029 Human
- SwissProt: O15455 Human
- SwissProt: Q99MB1 Mouse
- Unigene: 657724 Human
- Unigene: 33874 Mouse
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别名
- CD283 antibody
- CD283 antigen antibody
- IIAE2 antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TLR3 antibody (ab62566)
Paraffin-embedded mouse spleen tissue stained for TLR3 using ab62566 at 2 µg/ml in immunohistochemical analysis. DAB detection. Counterstained with hematoxylin.
Antigen Retrieval (Microwave Method).
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Western blot - Anti-TLR3 antibody (ab62566)Western blot analysis of TLR3 expression in mouse spleen tissue lysate with ab62566 at (A) 0.5 and (B) 1µg/ml. -
Western blot - Anti-TLR3 antibody (ab62566)Lane 1 : Anti-TLR3 antibody (ab62566) at 1 µg/ml
Lane 2 : Anti-TLR3 antibody (ab62566) at 2 µg/ml
All lanes : Daudi (Human Burkitt's lymphoma cell line) cell lysate
Lysates/proteins at 15 µg per lane.
Predicted band size: 104 kDa
Observed band size: 104 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TLR3 antibody (ab62566)Paraffin-embedded human spleen tissue stained for TLR3 using ab62566 at 5 µg/ml in immunohistochemical analysis. DAB detection. Counterstained with hematoxylin.
Antigen Retrieval (Microwave Method).
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Immunocytochemistry - Anti-TLR3 antibody (ab62566)Immunocytochemical analysis of human spleen tissue labeling TLR3 using ab62566 at 20 µg/ml (green).
Antigen Retrieval (Microwave Method).
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (50)
ab62566 被引用在 50 文献中.
- Ju C et al. Photobiomodulation promotes spinal cord injury repair by inhibiting macrophage polarization through lncRNA TUG1-miR-1192/TLR3 axis. Cell Mol Biol Lett 28:5 (2023). PubMed: 36658478
- Bhagwani AR et al. A p53-TLR3 axis ameliorates pulmonary hypertension by inducing BMPR2 via IRF3. iScience 26:105935 (2023). PubMed: 36685041
- Lye P et al. Effects of bacterial and viral pathogen-associated molecular patterns (PAMPs) on multidrug resistance (MDR) transporters in brain endothelial cells of the developing human blood-brain barrier. Fluids Barriers CNS 20:8 (2023). PubMed: 36721242
- Fan L et al. High expression of TLR3 in triple-negative breast cancer predicts better prognosis-data from the Fudan University Shanghai Cancer Center cohort and tissue microarrays. BMC Cancer 23:298 (2023). PubMed: 37005579
- Zhang X et al. Extracellular RNAs-TLR3 signaling contributes to cognitive impairment after chronic neuropathic pain in mice. Signal Transduct Target Ther 8:292 (2023). PubMed: 37544956