Anti-TLR2抗体[TL2.1] (ab9100)
![Flow Cytometry - Anti-TLR2 antibody [TL2.1] (ab9100)](https://www.abcam.cn/ps/products/9/ab9100/Images/ab9100-16776-anti-tlr2-antibody-tl21-flow-cytometry.jpg "Flow Cytometry - Anti-TLR2 antibody [TL2.1] (ab9100)")
Key features and details
- Mouse monoclonal [TL2.1] to TLR2
- Suitable for: IP, Functional Studies, Flow Cyt, WB, Neutralising, ICC/IF
- Reacts with: Human, Common marmoset
- Isotype: IgG2a
Related conjugates and formulations
概述
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产品名称
Anti-TLR2抗体[TL2.1]
参阅全部 TLR2 一抗 -
描述
小鼠单克隆抗体[TL2.1] to TLR2 -
宿主
Mouse -
经测试应用
适用于: IP, Functional Studies, Flow Cyt, WB, Neutralising, ICC/IFmore details -
种属反应性
与反应: Human, Common marmoset
预测可用于: Dog, Monkey, Cynomolgus monkey, Rhesus monkey
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免疫原
Recombinant human TLR2 produced by the CHO/CD14 reporter cell line.
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阳性对照
- WB: THP1 + LPS. IHC-P: Human TLR2. ICC: Human U937 cells. Flow: Human peripheral blood mononuclear cells.
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常规说明
Ab9100 is a TLR2 function blocking antibody that is useful for studies on the role of TLR2 as a pattern recognition receptor in microbial products induced cytokine production by TLR2 bearing cells such as human pheriperal blood mononuclear cells.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
Constituents: PBS, 1% BSA -
Concentration information loading... -
纯度
Protein G purified -
纯化说明
0.2 µm filtered -
Primary antibody说明
Ab9100 is a TLR2 function blocking antibody that is useful for studies on the role of TLR2 as a pattern recognition receptor in microbial products induced cytokine production by TLR2 bearing cells such as human pheriperal blood mononuclear cells. -
克隆
单克隆 -
克隆编号
TL2.1 -
同种型
IgG2a -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IP |
Use at an assay dependent concentration.
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| Functional Studies |
Use at an assay dependent concentration.
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| Flow Cyt | (1) |
Use 2-3µg for 105 cells.
Untreated and 0.4 % formaldehyde fixed cells can be used; do not permeabilize the cells. ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
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| WB | (3) |
1/50.
Abcam recommends using non-reducing conditions. |
| Neutralising |
Use at an assay dependent concentration.
See Lien et al. |
|
| ICC/IF | (1) |
Use at an assay dependent concentration.
Fix with PBS/2% formalin for 15 min at 4 degree C; permeabilize with acetone, 10 min, -20 degree C (see Flo et al J Leukoc Biol which used a conjugated form of the antibody). |
| 说明 |
|---|
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IP
Use at an assay dependent concentration. |
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Functional Studies
Use at an assay dependent concentration. |
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Flow Cyt
Use 2-3µg for 105 cells. Untreated and 0.4 % formaldehyde fixed cells can be used; do not permeabilize the cells. ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
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WB
1/50. Abcam recommends using non-reducing conditions. |
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Neutralising
Use at an assay dependent concentration. See Lien et al. |
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ICC/IF
Use at an assay dependent concentration. Fix with PBS/2% formalin for 15 min at 4 degree C; permeabilize with acetone, 10 min, -20 degree C (see Flo et al J Leukoc Biol which used a conjugated form of the antibody). |
靶标
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功能
Cooperates with LY96 to mediate the innate immune response to bacterial lipoproteins and other microbial cell wall components. Cooperates with TLR1 to mediate the innate immune response to bacterial lipoproteins or lipopeptides. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. May also promote apoptosis in response to lipoproteins. Recognizes mycoplasmal macrophage-activating lipopeptide-2kD (MALP-2), soluble tuberculosis factor (STF), phenol-soluble modulin (PSM) and B.burgdorferi outer surface protein A lipoprotein (OspA-L) cooperatively with TLR6. -
组织特异性
Highly expressed in peripheral blood leukocytes, in particular in monocytes, in bone marrow, lymph node and in spleen. Also detected in lung and in fetal liver. Levels are low in other tissues. -
序列相似性
Belongs to the Toll-like receptor family.
Contains 14 LRR (leucine-rich) repeats.
Contains 1 TIR domain. -
翻译后修饰
Glycosylation of Asn-442 is critical for secretion of the N-terminal ectodomain of TLR2. -
细胞定位
Membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 448807 Dog
- Entrez Gene: 7097 Human
- Entrez Gene: 574290 Rhesus monkey
- Omim: 603028 Human
- SwissProt: Q95M53 Cynomolgus monkey
- SwissProt: Q689D1 Dog
- SwissProt: O60603 Human
- SwissProt: B3Y618 Rhesus monkey
see all -
别名
- CD282 antibody
- CD282 antigen antibody
- TIL 4 antibody
see all
图片
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Flow Cytometry - Anti-TLR2 antibody [TL2.1] (ab9100)TLR2 staining on human peripheral blood mononuclear cells by using ab9100. Green: CD3+-cells; Red: CD14+ cells
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![Western blot - Anti-TLR2 antibody [TL2.1] (ab9100)](https://www.abcam.cn/ps/products/9/ab9100/Images/ab9100-256365-anti-tlr2-antibody-tl21-western-blot.jpg)
Western blot - Anti-TLR2 antibody [TL2.1] (ab9100)All lanes : Anti-TLR2 antibody [TL2.1] (ab9100) at 1 µg/ml
Lane 1 : THP1
Lane 2 : THP1 + LPS
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/50000 dilution
Performed under reducing conditions.This blot was produced using a 4-12% Bis-Tris gel under the MOPS buffer system. The gel was ran at 200V for 60 minutes before being transferred onto a nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked with 2% BSA for 60 minutes before being incubated with ab9100 (anti-TLR2 antibody; 1 ug/mL) for 18 hours at 4°C. Antibody binding was detected using ab97040 (HRP-labelled goat anti-mouse IgG) at 1:50,000 dilutions for 1 hour at room temperature.Visualisation was conducted using ECL development solution ab133406.
This gel was run under reducing conditions resulting in weak signal. We recommend customers to use non-reducing conditions.
数据表及文件
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Datasheet download
文献 (37)
ab9100 被引用在 37 文献中.
- Yan Z et al. TREM1 is involved in the mechanism between asthma and lung cancer by regulating the Toll‑like receptor signaling pathway. Oncol Lett 27:16 (2024). PubMed: 38028174
- Regueiro U et al. Immunomodulatory Effect of Human Lactoferrin on Toll-like Receptors 2 Expression as Therapeutic Approach for Keratoconus. Int J Mol Sci 23:N/A (2022). PubMed: 36293206
- Wu S et al. The GAR/RGG motif defines a family of nuclear alarmins. Cell Death Dis 12:477 (2021). PubMed: 33980825
- Luo CL et al. RBFOX2/GOLIM4 Splicing Axis Activates Vesicular Transport Pathway to Promote Nasopharyngeal Carcinogenesis. Adv Sci (Weinh) 8:e2004852 (2021). PubMed: 34180133
- Schlichtner S et al. Functional role of galectin-9 in directing human innate immune reactions to Gram-negative bacteria and T cell apoptosis. Int Immunopharmacol 100:108155 (2021). PubMed: 34543981
