JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB9100

Anti-TLR2抗体[TL2.1]

Anti-TLR2 antibody [TL2.1]

4

(9 Reviews)

|

(40 Publications)

Mouse Monoclonal TLR2 antibody. Suitable for IP, Flow Cyt, WB, FuncS, FuncS (Neut/Block), ICC/IF and reacts with Common marmoset, Cynomolgus monkey, Rhesus monkey, Human, Dog samples. Cited in 40 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human TLR2.

查看别名

CD282, TIL4, TLR2, Toll-like receptor 2, Toll/interleukin-1 receptor-like protein 4

2 Images
Flow Cytometry - Anti-TLR2 antibody [TL2.1] (AB9100)
  • Flow Cyt

Unknown

Flow Cytometry - Anti-TLR2 antibody [TL2.1] (AB9100)

TLR2 staining on human peripheral blood mononuclear cells by using ab9100. Green : CD3+-cells; Red : CD14+ cells

Western blot - Anti-TLR2 antibody [TL2.1] (AB9100)
  • WB

Lab

Western blot - Anti-TLR2 antibody [TL2.1] (AB9100)

This blot was produced using a 4-12% Bis-Tris gel under the MOPS buffer system. The gel was ran at 200V for 60 minutes before being transferred onto a nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked with 2% BSA for 60 minutes before being incubated with ab9100 (anti-TLR2 antibody; 1 ug/mL) for 18 hours at 4°C. Antibody binding was detected using ab97040 (HRP-labelled goat anti-mouse IgG) at 1 : 50,000 dilutions for 1 hour at room temperature.Visualisation was conducted using ECL development solution ab133406.

This gel was run under reducing conditions resulting in weak signal. We recommend customers to use non-reducing conditions.

All lanes:

Western blot - Anti-TLR2 antibody [TL2.1] (ab9100) at 1 µg/mL

Lane 1:

THP1 at 20 µg

Lane 2:

THP1 + LPS at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Mouse IgG H&L (HRP) preadsorbed (<a href='/products/secondary-antibodies/goat-mouse-igg-h-l-hrp-preadsorbed-ab97040'>ab97040</a>) at 1/50000 dilution

Predicted band size: 89 kDa

false

关键信息

宿主种属

Mouse

克隆

Monoclonal

克隆号

TL2.1

亚型

IgG2a

不含载体蛋白

No

反应种属

Human, Common marmoset

应用

Flow Cyt, WB, IP, FuncS (Neut/Block), ICC/IF, FuncS

applications

免疫原

Recombinant Full Length Protein corresponding to Human TLR2.

O60603

反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FuncS" : {"fullname" : "Functional Studies", "shortname":"FuncS"}, "FuncSNeutBlock" : {"fullname" : "Functional Studies (Neut/Block)", "shortname":"FuncS (Neut/Block)"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "2-3 µg for 10^5 Cells", "FlowCyt-species-notes": "<p>Untreated and 0.4 % formaldehyde fixed cells can be used; do not permeabilize the cells.</p><p><a href='/products/primary-antibodies/mouse-igg2c-kappa-monoclonal-18c8bc7ad10-isotype-control-ab170191'>ab170191</a> - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.</p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/50", "WB-species-notes": "<p>Abcam recommends using non-reducing conditions.</p>", "FuncS-species-checked": "guaranteed", "FuncS-species-dilution-info": "", "FuncS-species-notes": "<p></p>", "FuncSNeutBlock-species-checked": "guaranteed", "FuncSNeutBlock-species-dilution-info": "", "FuncSNeutBlock-species-notes": "<p>See Lien et al.</p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p>Fix with PBS/2% formalin for 15 min at 4 degree C; permeabilize with acetone, 10 min, -20 degree C (see Flo et al J Leukoc Biol which used a conjugated form of the antibody).</p>" }, "Common marmoset": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "FuncS-species-checked": "guaranteed", "FuncS-species-dilution-info": "", "FuncS-species-notes": "<p></p>", "FuncSNeutBlock-species-checked": "guaranteed", "FuncSNeutBlock-species-dilution-info": "", "FuncSNeutBlock-species-notes": "<p>See Lien et al.</p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p>Fix with PBS/2% formalin for 15 min at 4 degree C; permeabilize with acetone, 10 min, -20 degree C (see Flo et al J Leukoc Biol which used a conjugated form of the antibody).</p>" }, "Cynomolgus monkey": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "FuncS-species-checked": "guaranteed", "FuncS-species-dilution-info": "", "FuncS-species-notes": "<p></p>", "FuncSNeutBlock-species-checked": "guaranteed", "FuncSNeutBlock-species-dilution-info": "", "FuncSNeutBlock-species-notes": "<p>See Lien et al.</p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p>Fix with PBS/2% formalin for 15 min at 4 degree C; permeabilize with acetone, 10 min, -20 degree C (see Flo et al J Leukoc Biol which used a conjugated form of the antibody).</p>" }, "Dog": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "FuncS-species-checked": "guaranteed", "FuncS-species-dilution-info": "", "FuncS-species-notes": "<p></p>", "FuncSNeutBlock-species-checked": "guaranteed", "FuncSNeutBlock-species-dilution-info": "", "FuncSNeutBlock-species-notes": "<p>See Lien et al.</p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p>Fix with PBS/2% formalin for 15 min at 4 degree C; permeabilize with acetone, 10 min, -20 degree C (see Flo et al J Leukoc Biol which used a conjugated form of the antibody).</p>" }, "Rhesus monkey": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "FuncS-species-checked": "guaranteed", "FuncS-species-dilution-info": "", "FuncS-species-notes": "<p></p>", "FuncSNeutBlock-species-checked": "guaranteed", "FuncSNeutBlock-species-dilution-info": "", "FuncSNeutBlock-species-notes": "<p>See Lien et al.</p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p>Fix with PBS/2% formalin for 15 min at 4 degree C; permeabilize with acetone, 10 min, -20 degree C (see Flo et al J Leukoc Biol which used a conjugated form of the antibody).</p>" } } }

产品详情

ab9100 is a TLR2 function blocking antibody that is useful for studies on the role of TLR2 as a pattern recognition receptor in microbial products induced cytokine production by TLR2 bearing cells such as human pheriperal blood mononuclear cells.

性能和储存信息

形式
Liquid
纯化工艺
Affinity purification Protein G
纯化说明
0.2 µm filtered
存储溶液
Constituents: PBS, 1% BSA
运输条件
Blue Ice
推荐的短期储存条件
+4°C
推荐的长期储存条件
-20°C
分装信息
Upon delivery aliquot
储存信息
Avoid freeze / thaw cycle

补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Toll-like receptor 2 (TLR2) also called CD282 is a protein that detects molecules from bacteria and fungi. It has a mass of about 90 kDa. TLR2 is expressed on the surface of immune cells such as monocytes macrophages and neutrophils. This receptor recognizes pathogen-associated molecular patterns (PAMPs) and activates the immune response.
Biological function summary

TLR2 initiates signaling pathways that lead to the production of cytokines and chemokines helping to control infection. It forms dimers with other TLRs like TLR1 or TLR6 to expand its recognition range of microbial components. TLR2's activity is important for balancing pathogen recognition and avoiding overactive immune response. On macrophages TLR2 activation enhances their phagocytic ability.

Pathways

TLR2 plays roles in innate immune signaling pathways and inflammation pathways. It contributes significantly to the NF-kB and MAPK pathways which regulate inflammation and immune response. TLR2 often cooperates with proteins like MyD88 and TRAF6 in these pathways leading to downstream activation of transcription factors and production of pro-inflammatory molecules.

TLR2 is associated with inflammatory and infectious diseases including sepsis and tuberculosis. During tuberculosis interactions between TLR2 and its ligands trigger immune responses that are important for bacterial control. Additionally TLR2’s overactivation links to sepsis by causing excessive inflammation. Researchers study TLR2 for potential therapeutic targeting in these diseases assessing how modulation can improve outcomes or reduce detrimental inflammation.

产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

靶点信息

Cooperates with LY96 to mediate the innate immune response to bacterial lipoproteins and other microbial cell wall components. Cooperates with TLR1 or TLR6 to mediate the innate immune response to bacterial lipoproteins or lipopeptides (PubMed : 17889651, PubMed : 21078852). Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. May also activate immune cells and promote apoptosis in response to the lipid moiety of lipoproteins (PubMed : 10426995, PubMed : 10426996). Recognizes mycoplasmal macrophage-activating lipopeptide-2kD (MALP-2), soluble tuberculosis factor (STF), phenol-soluble modulin (PSM) and B.burgdorferi outer surface protein A lipoprotein (OspA-L) cooperatively with TLR6 (PubMed : 11441107). Stimulation of monocytes in vitro with M.tuberculosis PstS1 induces p38 MAPK and ERK1/2 activation primarily via this receptor, but also partially via TLR4 (PubMed : 16622205). MAPK activation in response to bacterial peptidoglycan also occurs via this receptor (PubMed : 16622205). Acts as a receptor for M.tuberculosis lipoproteins LprA, LprG, LpqH and PstS1, some lipoproteins are dependent on other coreceptors (TLR1, CD14 and/or CD36); the lipoproteins act as agonists to modulate antigen presenting cell functions in response to the pathogen (PubMed : 19362712). M.tuberculosis HSP70 (dnaK) but not HSP65 (groEL-2) acts via this protein to stimulate NF-kappa-B expression (PubMed : 15809303). Recognizes M.tuberculosis major T-antigen EsxA (ESAT-6) which inhibits downstream MYD88-dependent signaling (shown in mouse) (By similarity). Forms activation clusters composed of several receptors depending on the ligand, these clusters trigger signaling from the cell surface and subsequently are targeted to the Golgi in a lipid-raft dependent pathway. Forms the cluster TLR2 : TLR6 : CD14 : CD36 in response to diacylated lipopeptides and TLR2 : TLR1 : CD14 in response to triacylated lipopeptides (PubMed : 16880211). Required for normal uptake of M.tuberculosis, a process that is inhibited by M.tuberculosis LppM (By similarity).
See full target information TLR2

文献 (40)

Recent publications for all applications. Explore the full list and refine your search

Respiratory research 26:131 PubMed40205554

2025

Early secretory antigen target of 6-kDa of Mycobacterium tuberculosis inhibits macrophage apoptosis and host defense via TLR2.

Applications

Unspecified application

Species

Unspecified reactive species

Lin Zhang,Fang Fang,Danrui Liu,Geman Xia,Tong Feng,Jingzhu Lv,Jinying Qi,Tengteng Li,Hui Liu,Tao Xu,Fengjiao Wu,Chuanwang Song,Wei Li,Xiaojing Wang,Xianyou Chang,Hongtao Wang,Ting Wang,Zhongqing Qian

Materials today. Bio 31:101484 PubMed39925716

2025

Janus adhesive bio-patches with targeted drug delivery enabled anti-bacteria and pro-angiogenesis for dura mater repair.

Applications

Unspecified application

Species

Unspecified reactive species

Yirizhati Aili,Pengfei Wei,Xueqiao Yu,Guofeng Fan,Nuerailijiang Maimaitiaili,Yunhuan Li,Siqi Liu,Yiqian Huang,Bo Zhao,Zengliang Wang,Hu Qin,Yongxin Wang

Oncology letters 27:16 PubMed38028174

2023

TREM1 is involved in the mechanism between asthma and lung cancer by regulating the Toll‑like receptor signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Zhulin Yan,Wei Zhang,Keyu Sun

International journal of molecular sciences 23: PubMed36293206

2022

Immunomodulatory Effect of Human Lactoferrin on Toll-like Receptors 2 Expression as Therapeutic Approach for Keratoconus.

Applications

Unspecified application

Species

Unspecified reactive species

Uxía Regueiro,Maite López-López,Rubén Varela-Fernández,Tomás Sobrino,Elio Diez-Feijoo,Isabel Lema

International immunopharmacology 100:108155 PubMed34543981

2021

Functional role of galectin-9 in directing human innate immune reactions to Gram-negative bacteria and T cell apoptosis.

Applications

Unspecified application

Species

Unspecified reactive species

Stephanie Schlichtner,N Helge Meyer,Inna M Yasinska,Nijas Aliu,Steffen M Berger,Bernhard F Gibbs,Elizaveta Fasler-Kan,Vadim V Sumbayev

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 8:e2004852 PubMed34180133

2021

RBFOX2/GOLIM4 Splicing Axis Activates Vesicular Transport Pathway to Promote Nasopharyngeal Carcinogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Chun-Ling Luo,Xiao-Chen Xu,Chu-Jun Liu,Shuai He,Jie-Rong Chen,Yan-Chun Feng,Shu-Qiang Liu,Wan Peng,Ya-Qing Zhou,Yu-Xiang Liu,Pan-Pan Wei,Bo Li,Hai-Qiang Mai,Xiao-Jun Xia,Jin-Xin Bei

Cell death & disease 12:477 PubMed33980825

2021

The GAR/RGG motif defines a family of nuclear alarmins.

Applications

Unspecified application

Species

Unspecified reactive species

Shan Wu,Boon Heng Dennis Teo,Seng Yin Kelly Wee,Junjie Chen,Jinhua Lu

American journal of reproductive immunology (New York, N.Y. : 1989) 85:e13370 PubMed33152143

2020

Effects of a gestational level of estradiol on cellular transition, migration, and inflammation in cervical epithelial and stromal cells.

Applications

Unspecified application

Species

Unspecified reactive species

Ourlad Alzeus G Tantengco,Lauren S Richardson,Ramkumar Menon

Journal of neuroimmune pharmacology : the official 14:688-696 PubMed31321663

2019

Neuroprotective Effects of Genistein in a SOD1-G93A Transgenic Mouse Model of Amyotrophic Lateral Sclerosis.

Applications

Unspecified application

Species

Unspecified reactive species

Zichun Zhao,Jinsheng Fu,Shiping Li,Zhenzhong Li

PloS one 14:e0215234 PubMed30978209

2019

Retinoids induce antagonism between FOXO3A and FOXM1 transcription factors in human oral squamous cell carcinoma (OSCC) cells.

Applications

Unspecified application

Species

Unspecified reactive species

Kwame Osei-Sarfo,Lorraine J Gudas
View all publications

Abcam Product Promise

我们致力于为您的研究提供高质量的试剂,为您科研的每一步提供支持。若我们的产品未能达到预期性能,我们向您提供 Abcam Product Promise 保障。
详情请参阅我们的条款与条件。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com