重组Anti-TLR2抗体[EPR20303] - BSA and Azide free (ab271986)
![Immunoprecipitation - Anti-TLR2 antibody [EPR20303] - BSA and Azide free (ab271986)](https://www.abcam.cn/ps/products/271/ab271986/Images/ab271986-1-ab209217272119antitlr2antibodyepr20303immunoprecipitation.jpg "Immunoprecipitation - Anti-TLR2 antibody [EPR20303] - BSA and Azide free (ab271986)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR20303] to TLR2 - BSA and Azide free
- Suitable for: IP, WB
- Reacts with: Mouse
Related conjugates and formulations
概述
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产品名称
Anti-TLR2抗体[EPR20303] - BSA and Azide free
参阅全部 TLR2 一抗 -
描述
兔单克隆抗体[EPR20303] to TLR2 - BSA and Azide free -
宿主
Rabbit -
经测试应用
适用于: IP, WBmore details -
种属反应性
与反应: Mouse
预测可用于: Rat
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免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: Mouse and rat spleen lysates; RAW 264.7 whole cell lysates untreated and treated with 1 µg/ml lipopolysaccharides for 6 hours IP: RAW 264.7 treated with 1 µg/ml LPS for 6 hours whole cell lysate.
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常规说明
ab271986 is the carrier-free version of ab209217.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
存储溶液
pH: 7.2
Constituent: PBS -
无载体
是 -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR20303 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab271986于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IP |
Use at an assay dependent concentration.
|
|
| WB |
Use at an assay dependent concentration. Predicted molecular weight: 89 kDa.
|
| 说明 |
|---|
|
IP
Use at an assay dependent concentration. |
|
WB
Use at an assay dependent concentration. Predicted molecular weight: 89 kDa. |
靶标
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功能
Cooperates with LY96 to mediate the innate immune response to bacterial lipoproteins and other microbial cell wall components. Cooperates with TLR1 to mediate the innate immune response to bacterial lipoproteins or lipopeptides. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. May also promote apoptosis in response to lipoproteins. Recognizes mycoplasmal macrophage-activating lipopeptide-2kD (MALP-2), soluble tuberculosis factor (STF), phenol-soluble modulin (PSM) and B.burgdorferi outer surface protein A lipoprotein (OspA-L) cooperatively with TLR6. -
组织特异性
Highly expressed in peripheral blood leukocytes, in particular in monocytes, in bone marrow, lymph node and in spleen. Also detected in lung and in fetal liver. Levels are low in other tissues. -
序列相似性
Belongs to the Toll-like receptor family.
Contains 14 LRR (leucine-rich) repeats.
Contains 1 TIR domain. -
翻译后修饰
Glycosylation of Asn-442 is critical for secretion of the N-terminal ectodomain of TLR2. -
细胞定位
Membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 24088 Mouse
- Entrez Gene: 310553 Rat
- SwissProt: Q9QUN7 Mouse
- Unigene: 87596 Mouse
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别名
- CD282 antibody
- CD282 antigen antibody
- TIL 4 antibody
see all
图片
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Immunoprecipitation - Anti-TLR2 antibody [EPR20303] - BSA and Azide free (ab271986)
TLR2 was immunoprecipitated from 0.35 mg of RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate, treated with 1 µg/ml LPS for 6 hours, with ab209217 at 1/30 dilution.
Western blot was performed from the immunoprecipitate using ab209217 at 1/500 dilution.
VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.
Lane 1: RAW 264.7 whole cell lysate treated with 1 µg/ml LPS for 6 hours, 10 µg (Input).
Lane 2: ab209217 IP in RAW 264.7 whole cell lysate treated with 1 µg/ml LPS for 6 hours.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab209217 in RAW 264.7 whole cell lysate treated with 1 µg/ml LPS for 6 hours.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 1 second.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab209217). -
![Anti-TLR2 antibody [EPR20303] - BSA and Azide free (ab271986)](https://www.abcam.cn/ps/products/271/ab271986/Images/ab271986-2-benefits-of-recombinant-antibodies.png)
Anti-TLR2 antibody [EPR20303] - BSA and Azide free (ab271986)
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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Datasheet download
Certificate of Compliance
文献 (0)
ab271986 尚未被引用在任何文献中。
