AB213596

Anti-TLE3/ESG抗体[CL3573]

Name: TLE3/ESG抗体[CL3573] (ab213596)| Abcam中文官网
Brand: Abcam Limited
SKU: AB213596
Price: 4845 CNY
Availability: InStock

Anti-TLE3/ESG antibody [CL3573]

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(2 Publications)

Mouse Monoclonal TLE3/ESG antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human TLE3 aa 150 to C-terminus.

查看别名

KIAA1547, TLE3, Transducin-like enhancer protein 3, Enhancer of split groucho-like protein 3, ESG3

9 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TLE3/ESG antibody [CL3573] (AB213596)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TLE3/ESG antibody [CL3573] (AB213596)

Immunohistochemical analysis of Paraffin-embedded Human cervix tissue labeling TLE3/ESG with ab213596 at 1/10000 dilution.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TLE3/ESG antibody [CL3573] (AB213596)

Immunohistochemical analysis of Paraffin-embedded Human tonsil tissue labeling TLE3/ESG with ab213596 at 1/10000 dilution.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TLE3/ESG antibody [CL3573] (AB213596)

Immunohistochemical analysis of Paraffin-embedded Human small intestine tissue labeling TLE3/ESG with ab213596 at 1/10000 dilution.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TLE3/ESG antibody [CL3573] (AB213596)

Immunohistochemical analysis of Paraffin-embedded Human kidney tissue labeling TLE3/ESG with ab213596 at 1/10000 dilution.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TLE3/ESG antibody [CL3573] (AB213596)

Immunohistochemical analysis of Paraffin-embedded Human oral mucosa tissue labeling TLE3/ESG with ab213596 at 1/10000 dilution.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TLE3/ESG antibody [CL3573] (AB213596)

Immunohistochemical analysis of Paraffin-embedded Human skeletal muscle tissue labeling TLE3/ESG with ab213596 at 1/10000 dilution.

Immunocytochemistry/ Immunofluorescence - Anti-TLE3/ESG antibody [CL3573] (AB213596)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-TLE3/ESG antibody [CL3573] (AB213596)

PFA-fixed, Triton X-100 permeabilized HeLa cells stained for TLE3 (Green) using ab213596 at 4 μg/ml in ICC/IF.

Microtublues are stained red.

Supplier Data

Western blot - Anti-TLE3/ESG antibody [CL3573] (AB213596)

All lanes:

Western blot - Anti-TLE3/ESG antibody [CL3573] (ab213596) at 1/500 dilution

All lanes:

HEK293 cell lysate

Predicted band size: 83 kDa

false

Supplier Data

Western blot - Anti-TLE3/ESG antibody [CL3573] (AB213596)

All lanes:

Western blot - Anti-TLE3/ESG antibody [CL3573] (ab213596) at 1/500 dilution

All lanes:

HeLa nuclear lysate

Predicted band size: 83 kDa

false

关键信息

宿主种属

Mouse

克隆

Monoclonal

克隆号

CL3573

亚型

IgG1

不含载体蛋白

反应种属

Human

应用

IHC-P, ICC/IF, WB

applications

免疫原

Recombinant Fragment Protein within Human TLE3 aa 150 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q04726

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/10000 - 1/20000", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "2-10 µg/mL", "ICCIF-species-notes": "Fixation/Permeabilization: PFA/Triton X-100" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

TLE3 also known as ESG is a transcriptional corepressor with a molecular mass of around 80 kDa. It belongs to the transducin-like enhancer of split (TLE) family which interacts with DNA-binding proteins to modulate gene transcription. TLE3 is expressed mainly in the nucleus of cells and has notable expression in various tissues including the brain skeletal muscle and adipose tissues. Its mechanical role involves binding to nuclear receptors and influencing the transcriptional repression process by preventing the assembly of transcriptional activators on target genes.

Biological function summary

TLE3 serves as a critical modulator of differentiation pathways. It often functions as part of a broader repressor complex working closely with Groucho-related proteins. These complexes exert influence over cell lineage commitment and differentiation particularly in the context of adipogenesis and muscle development. TLE3 helps direct stem cell fate by repressing pro-differentiation signals maintaining cells in a less differentiated state until specific developmental cues trigger further differentiation.

Pathways

The function of TLE3 intersects with Wnt and Notch signaling pathways both essential for cellular growth and differentiation processes. TLE3 associates with proteins like TCF/LEF during Wnt signaling to suppress target gene activation therefore regulating cellular proliferation and differentiation. In the Notch pathway TLE3 interacts with the CSL transcription factor contributing to the repression of Notch-responsive genes which play a part in the maintenance of stem cell populations and tissue homeostasis.

TLE3 shows a connection with cancer and metabolic syndromes. In cancer aberrant expression of TLE3 can lead to disrupted cell differentiation promoting oncogenesis. For example its interaction with TCF/LEF proteins in the Wnt pathway can affect tumor suppressor gene expression. In metabolic disorders TLE3 impacts adipogenesis contributing to obesity-linked insulin resistance. Dysregulation in these pathways linked to proteins like PPARγ highlights TLE3's role in disease pathophysiology and potential as a therapeutic target.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Transcriptional coregulator that binds to a number of transcription factors. Acts as a regulator of adipogenesis both by acting as a corepressor of Wnt signaling and coactivator for PPARG (PubMed : 28689657). Inhibits the transcriptional activation mediated by CTNNB1 and TCF family members in Wnt signaling (PubMed : 28689657). Promotes diffentiation of white adipocytes by acting as a coactivator for PPARG and preventing the association between PRDM16 and PPARG, thereby preventing browning of white adipocytes (By similarity).

See full target information TLE3

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文献 (2)

Recent publications for all applications. Explore the full list and refine your search

Genes & development 33:747-762 PubMed31123067

2019

Loss of TLE3 promotes the mitochondrial program in beige adipocytes and improves glucose metabolism.

Applications

Unspecified application

Species

Unspecified reactive species

Stephanie Pearson,Anne Loft,Prashant Rajbhandari,Judith Simcox,Sanghoon Lee,Peter Tontonoz,Susanne Mandrup,Claudio J Villanueva

The Journal of biological chemistry 292:12885-12894 PubMed28607151

2017

The transcriptional co-repressor TLE3 regulates myogenic differentiation by repressing the activity of the MyoD transcription factor.

Applications

Unspecified application

Species

Unspecified reactive species

Shoichiro Kokabu,Chihiro Nakatomi,Takuma Matsubara,Yusuke Ono,William N Addison,Jonathan W Lowery,Mariko Urata,Aaron M Hudnall,Suzuro Hitomi,Mitsushiro Nakatomi,Tsuyoshi Sato,Kenji Osawa,Tetsuya Yoda,Vicki Rosen,Eijiro Jimi

View all publications

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Anti-TLE3/ESG抗体[CL3573]

Anti-TLE3/ESG antibody [CL3573]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TLE3/ESG antibody [CL3573] (AB213596)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TLE3/ESG antibody [CL3573] (AB213596)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TLE3/ESG antibody [CL3573] (AB213596)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TLE3/ESG antibody [CL3573] (AB213596)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TLE3/ESG antibody [CL3573] (AB213596)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TLE3/ESG antibody [CL3573] (AB213596)

Immunocytochemistry/ Immunofluorescence - Anti-TLE3/ESG antibody [CL3573] (AB213596)

Western blot - Anti-TLE3/ESG antibody [CL3573] (AB213596)

Western blot - Anti-TLE3/ESG antibody [CL3573] (AB213596)

关键信息

宿主种属

克隆

克隆号

亚型

不含载体蛋白

反应种属

应用

免疫原