重组Anti-TL1A抗体[EPR23330-2] - BSA and Azide free (ab272051)
![Immunoprecipitation - Anti-TL1A antibody [EPR23330-2] - BSA and Azide free (ab272051)](https://www.abcam.cn/ps/products/272/ab272051/Images/ab272051-1-anti-tl1a-antibody-epr233302-immunoprecipitation-ht29.jpg "Immunoprecipitation - Anti-TL1A antibody [EPR23330-2] - BSA and Azide free (ab272051)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR23330-2] to TL1A - BSA and Azide free
- Suitable for: WB, IP
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-TL1A抗体[EPR23330-2] - BSA and Azide free
参阅全部 TL1A 一抗 -
描述
兔单克隆抗体[EPR23330-2] to TL1A - BSA and Azide free -
宿主
Rabbit -
经测试应用
适用于: WB, IPmore details
不适用于: Flow Cyt,ICC/IF or IHC-P -
种属反应性
与反应: Human -
免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: HUVEC, HUVEC (treated with/ml TNF-alpha for 24 hours) and HT-29 whole cell lysates. IP: HT-29 whole cell lysate.
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常规说明
ab272051 is the carrier-free version of ab270456.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
存储溶液
pH: 7.2
Constituent: PBS -
无载体
是 -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR23330-2 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab272051于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
Use at an assay dependent concentration. Detects a band of approximately 25-36 kDa (predicted molecular weight: 28 kDa).
|
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| IP |
Use at an assay dependent concentration.
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| 说明 |
|---|
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WB
Use at an assay dependent concentration. Detects a band of approximately 25-36 kDa (predicted molecular weight: 28 kDa). |
|
IP
Use at an assay dependent concentration. |
靶标
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功能
Receptor for TNFRSF25 and TNFRSF6B. Mediates activation of NF-kappa-B. Inhibits vascular endothelial growth and angiogenesis (in vitro). Promotes activation of caspases and apoptosis. -
组织特异性
Specifically expressed in endothelial cells. Detected in monocytes, placenta, lung, liver, kidney, skeletal muscle, pancreas, spleen, prostate, small intestine and colon. -
序列相似性
Belongs to the tumor necrosis factor family. -
细胞定位
Secreted and Membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 9966 Human
- Omim: 604052 Human
- SwissProt: O95150 Human
- Unigene: 241382 Human
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别名
- MGC129934 antibody
- MGC129935 antibody
- TL1 A antibody
see all
图片
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Immunoprecipitation - Anti-TL1A antibody [EPR23330-2] - BSA and Azide free (ab272051)
TL1A was immunoprecipitated from 0.35 mg HT-29 (human colorectal adenocarcinoma epithelial cell), whole cell lysate with ab270456 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab270456 at 270456 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/1000 dilution.
Lane 1: HT-29 (human colorectal adenocarcinoma epithelial cell), whole cell lysate 10 ug
Lane 2: ab270456 IP in HT-29 whole cell lysate
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab270456 in mouse ovary tissue lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 32 seconds
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab270456).
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![Anti-TL1A antibody [EPR23330-2] - BSA and Azide free (ab272051)](https://www.abcam.cn/ps/products/272/ab272051/Images/ab272051-2-benefits-of-recombinant-antibodies.png)
Anti-TL1A antibody [EPR23330-2] - BSA and Azide free (ab272051)
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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Datasheet download
Certificate of Compliance
文献 (0)
ab272051 尚未被引用在任何文献中。
