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AB274358

重组Anti-TL1A抗体[EPR23330-106] - BSA and Azide free (Capture)

Anti-TL1A antibody [EPR23330-106] - BSA and Azide free (Capture)

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Anti-TL1A antibody [EPR23330-106] - BSA and Azide free (Capture) (ab274358) is a rabbit recombinant monoclonal antibody provided in a PBS only buffer for easy conjugation detecting TL1A in ELISA. Suitable for Human.

- BSA, sodium azide, and glycerol-free for easy conjugation
- Biophysical QC for unrivalled batch-batch consistency

查看别名

TL1, VEGI, TNFSF15, Tumor necrosis factor ligand superfamily member 15, TNF ligand-related molecule 1, Vascular endothelial cell growth inhibitor

1 Images
Sandwich ELISA - Anti-TL1A antibody [EPR23330-106] - BSA and Azide free (Capture) (AB274358)
  • sELISA

Lab

Sandwich ELISA - Anti-TL1A antibody [EPR23330-106] - BSA and Azide free (Capture) (AB274358)

Sandwich ELISA of ab253542 with the capture antibody ab274358 dilution at 2 µg/mL and detector antibody ab274361 dilution at 0.5 µg/mL.

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR23330-106

亚型

IgG

不含载体蛋白

Yes

反应种属

Human

应用

sELISA

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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搭配使用产品

Assay Kits

AB317779

Human TL1A ELISA Kit- Extracellular

SimpleStep|Recombinant

Sandwich ELISA - Human TL1A ELISA Kit- Extracellular (AB317779)

  • 0
  • 0
Sample Prep & Detection Kits

AB102859

APC/Cy7® Conjugation Kit - Lightning-Link®

Flow Cytometry - APC/Cy7® Conjugation Kit - Lightning-Link® (AB102859)

  • 5
  • 1
Sample Prep & Detection Kits

AB188290

Gold-Maleimide Conjugation Kit (40nm, 20 OD)

Lateral flow assay - Gold-Maleimide Conjugation Kit (40nm, 20 OD) (AB188290)

  • 0
  • 0
Primary Antibodies

AB285134

Anti-LIGHT/TNFSF14 antibody [EPR23428-374] - BSA and Azide free (Detector)

RabMAb|Recombinant

Sandwich ELISA - Anti-LIGHT/TNFSF14 antibody [EPR23428-374] - BSA and Azide free (Detector) (AB285134)

  • 0
  • 0
Primary Antibodies

AB242692

Anti-GFAP antibody [EPR19996-132] - BSA and Azide free (Capture)

RabMAb|Recombinant

Sandwich ELISA - Anti-GFAP antibody [EPR19996-132] - BSA and Azide free (Capture) (AB242692)

  • 0
  • 0
Primary Antibodies

AB314487

Anti-IL-23 antibody [EPR26364-40] - BSA and Azide free

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-IL-23 antibody [EPR26364-40] - BSA and Azide free (AB314487)

  • 0
  • 0
Primary Antibodies

AB242514

Anti-Soluble TNF Receptor I antibody [EPR18822-47] - BSA and Azide free (Capture)

RabMAb|Recombinant

Sandwich ELISA - Anti-Soluble TNF Receptor I antibody [EPR18822-47] - BSA and Azide free (Capture) (AB242514)

  • 0
  • 0
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反应性数据

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产品详情

ab274358 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify human TNFSF15. The recommended pair for sandwich ELISA is:
Capture: ab274358, human TNFSF15 Capture Antibody (unconjugated)
Detector: ab274361, human TNFSF15 Detector Antibody (unconjugated)
The reference range value is 31.25-2000 pg/mL.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Sandwich ELISA
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

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性能和储存信息

形式
Liquid
纯化工艺
Affinity purification Protein A
存储溶液
pH: 7.2 - 7.4 Constituents: PBS
运输条件
Blue Ice
推荐的短期储存条件
+4°C
推荐的长期储存条件
+4°C
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

The protein TL1A also known as TNF-like ligand 1A is part of the tumor necrosis factor (TNF) superfamily. It is a cytokine with a molecular weight of approximately 23 kDa. Cells such as endothelial cells monocytes and fibroblasts express TL1A. TL1A predominantly acts as ligand for death receptor 3 (DR3) also called TNFRSF25 and is involved in immune response regulation. This protein can be found mainly in tissues involved in immune surveillance like lymph nodes and the gastrointestinal tract.
Biological function summary

TL1A interacts to induce T cell proliferation and cytokine production. It forms a complex with DR3 to activate downstream signaling pathways. These activities enhance adaptive immunity by stimulating T helper cells. Furthermore TL1A influences the production of inflammatory cytokines like interleukin-2 (IL-2) and tumor necrosis factor-alpha (TNF-α). Through its interaction with DR3 TL1A modulates important immune responses.

Pathways

TL1A features in the TNF receptor-mediated signaling and the NF-kB signaling pathway. In these pathways it links to proteins such as RIPK1 and TRAF2. The signaling through DR3 influences cell survival differentiation and apoptosis. These pathways are important in maintaining the balance between immune activation and tolerance. By interacting with various signaling molecules TL1A orchestrates complex immune functions that ensure adequate immune responses and prevent autoimmunity.

Researchers connect TL1A with conditions like inflammatory bowel disease (IBD) and rheumatoid arthritis (RA). These conditions involve chronic inflammation and immune dysregulation where TL1A plays a role. In IBD elevated TL1A levels drive inflammation in the intestines. In RA TL1A potentially interacts with fibroblast-like synoviocytes contributing to joint inflammation and damage. Its relation to DR3 further implicates TL1A in these autoimmune disorders making it a target for therapeutic interventions aiming to mitigate immune-mediated damage.
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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:
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靶点信息

Receptor for TNFRSF25 and TNFRSF6B. Mediates activation of NF-kappa-B. Inhibits vascular endothelial growth and angiogenesis (in vitro). Promotes activation of caspases and apoptosis.
See full target information TNFSF15
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