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重组RabMAb

重组Anti-Tissue Plasminogen活化剂抗体[EPR20233-226] - BSA and Azide free (Capture) (ab281237)

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Sandwich ELISA - Anti-Tissue Plasminogen Activator antibody [EPR20233-226] - BSA and Azide free (Capture) (ab281237)
  • Anti-Tissue Plasminogen Activator antibody [EPR20233-226] - BSA and Azide free (Capture) (ab281237)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR20233-226] to Tissue Plasminogen Activator - BSA and Azide free (Capture)
  • Suitable for: Sandwich ELISA
  • Reacts with: Human

概述

  • 产品名称

    Anti-Tissue Plasminogen活化剂抗体[EPR20233-226] - BSA and Azide free (Capture)
    参阅全部 Tissue Plasminogen Activator 一抗

  • 描述

    兔单克隆抗体[EPR20233-226] to组织Plasminogen活化剂- BSA and Azide free (Capture)

  • 宿主

    Rabbit

  • 经测试应用

    适用于: Sandwich ELISAmore details

  • 种属反应性

    与反应: Human

  • 免疫原

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • 常规说明

    ab281237 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Human Tissue Plasminogen Activator (TPA). The recommended pair for sandwich ELISA is:
         Capture: ab281237, Human Tissue Plasminogen Activator (TPA) Capture Antibody (unconjugated)
         Detector: ab281087, Human Tissue Plasminogen Activator (TPA) Detector Antibody (unconjugated)
    The reference range value is 78 - 5000 pg/mL.

    Alternatively, you can use:
         Capture: ab281237, Human PAI1 + tPA Complex Capture Antibody (unconjugated)
         Detector:ab269409, Human PAI1 + tPA Complex Detector Antibody (unconjugated)
    The reference range value is 78 - 5,000 pg/mL.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

性能

靶标

  • 功能

    Converts the abundant, but inactive, zymogen plasminogen to plasmin by hydrolyzing a single Arg-Val bond in plasminogen. By controlling plasmin-mediated proteolysis, it plays an important role in tissue remodeling and degradation, in cell migration and many other physiopathological events. Play a direct role in facilitating neuronal migration.

  • 组织特异性

    Synthesized in numerous tissues (including tumors) and secreted into most extracellular body fluids, such as plasma, uterine fluid, saliva, gingival crevicular fluid, tears, seminal fluid, and milk.

  • 疾病相关

    Note=Increased activity of TPA results in increased fibrinolysis of fibrin blood clots that is associated with excessive bleeding. Defective release of TPA results in hypofibrinolysis that can lead to thrombosis or embolism.

  • 序列相似性

    Belongs to the peptidase S1 family.
    Contains 1 EGF-like domain.
    Contains 1 fibronectin type-I domain.
    Contains 2 kringle domains.
    Contains 1 peptidase S1 domain.

  • 结构域

    Both FN1 and one of the kringle domains are required for binding to fibrin.
    Both FN1 and EGF-like domains are important for binding to LRP1.
    The FN1 domain mediates binding to annexin A2.
    The second kringle domain is implicated in binding to cytokeratin-8 and to the endothelial cell surface binding site.

  • 翻译后修饰

    The single chain, almost fully active enzyme, can be further processed into a two-chain fully active form by a cleavage after Arg-310 catalyzed by plasmin, tissue kallikrein or factor Xa.
    Differential cell-specific N-linked glycosylation gives rise to two glycoforms, type I (glycosylated at Asn-219) and type II (not glycosylated at Asn-219). The single chain type I glycoform is less readily converted into the two-chain form by plasmin, and the two-chain type I glycoform has a lower activity than the two-chain type II glycoform in the presence of fibrin.
    N-glycosylation of Asn-152; the bound oligomannosidic glycan is involved in the interaction with the mannose receptor.
    Characterization of O-linked glycan was studied in Bowes melanoma cell line.

  • 细胞定位

    Secreted > extracellular space.
  • Information by UniProt

  • 数据库链接

    • 别名

      • Alteplase antibody
      • DKFZp686I03148 antibody
      • Plasminogen activator tissue antibody
      • Plasminogen activator tissue type antibody
      • PLAT antibody
      • Reteplase antibody
      • t PA antibody
      • T Plasminogen Activator antibody
      • t-PA antibody
      • T-plasminogen activator antibody
      • Tissue plasminogen activator (t PA) antibody
      • Tissue type plasminogen activator antibody
      • Tissue-type plasminogen activator chain B antibody
      • tPA antibody
      • TPA_HUMAN antibody
      • TPA1 antibody
      see all

    图片

    • Sandwich ELISA - Anti-Tissue Plasminogen Activator antibody [EPR20233-226] - BSA and Azide free (Capture) (ab281237)
      Sandwich ELISA - Anti-Tissue Plasminogen Activator antibody [EPR20233-226] - BSA and Azide free (Capture) (ab281237)
      Example of Human Tissue Plasminogen Activator standard curve. Background-subtracted data values (mean +/- SD) are graphed.
    • Anti-Tissue Plasminogen Activator antibody [EPR20233-226] - BSA and Azide free (Capture) (ab281237)
      Anti-Tissue Plasminogen Activator antibody [EPR20233-226] - BSA and Azide free (Capture) (ab281237)

    实验方案

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    文献 (0)

    发表研究结果有使用 ab281237？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab281237 尚未被引用在任何文献中。

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