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AB244744

重组Anti-Tissue kallikrein抗体[EPR21764-41] - BSA and Azide free (Capture)

Anti-Tissue kallikrein antibody [EPR21764-41] - BSA and Azide free (Capture)

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Rabbit Recombinant Monoclonal Tissue kallikrein antibody. Carrier free. Suitable for sELISA and reacts with Mouse samples.

查看别名

Klk-6, Klk6, Klk1, Kallikrein-1, Glandular kallikrein K1, KAL-B, Renal kallikrein, Tissue kallikrein-6, mGK-6

1 Images
Sandwich ELISA - Anti-Tissue kallikrein antibody [EPR21764-41] - BSA and Azide free (Capture) (AB244744)
  • sELISA

Supplier Data

Sandwich ELISA - Anti-Tissue kallikrein antibody [EPR21764-41] - BSA and Azide free (Capture) (AB244744)

Representative standard curve from corresponding SimpleStep ELISA® Kit (ab233630).

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR21764-41

亚型

IgG

不含载体蛋白

Yes

反应种属

Mouse

应用

sELISA

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "sELISA" : {"fullname" : "Sandwich ELISA", "shortname":"sELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "sELISA-species-checked": "guaranteed", "sELISA-species-dilution-info": "", "sELISA-species-notes": "<p></p>" } } }

产品详情

ab244744 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Mouse Tissue kallikrein. The recommended pair for sandwich ELISA is:
Capture: ab244744, Mouse Tissue kallikrein Capture Antibody (unconjugated)
Detector: ab245014, Mouse Tissue kallikrein Detector Antibody (unconjugated)
The reference range value is 12.5 - 800 pg/ml.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Sandwich ELISA
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

性能和储存信息

形式
Liquid
纯化工艺
Affinity purification Protein A
存储溶液
Constituents: PBS
运输条件
Blue Ice
推荐的短期储存条件
+4°C
推荐的长期储存条件
+4°C

补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Tissue kallikrein also known as KLK1 is a serine protease enzyme that plays an important role in the cleavage of kininogen to produce kinins. This enzyme with a molecular weight of approximately 30 kDa exists in various tissues like the kidney pancreas and salivary glands. The protein is part of the kallikrein family and is expressed prominently in these areas. You can commonly measure its levels through ELISA assays labeled "kallikrein ELISA" or specific assays like the "kallikrein 3 ELISA."
Biological function summary

Tissue kallikrein contributes to vasodilation and blood pressure regulation by producing bradykinin a peptide with strong vasodilator properties. It does not function alone but may interact with other enzymes to amplify its effect. Tissue kallikrein belongs to a larger family of kallikrein-related peptidases which perform various physiological roles adding complexity to its functional network within the human body.

Pathways

This enzyme participates extensively in the kallikrein-kinin system. A significant interaction happens within the renin-angiotensin-aldosterone system contributing to blood pressure regulation fluid balance and cardiovascular function. It engages with related proteins such as kininogen and bradykinin establishing important checkpoints within these pathways.

Abnormalities in tissue kallikrein regulation link to hypertension and inflammatory diseases like rheumatoid arthritis. Dysregulation may alter interactions with proteins like kininogen leading to altered inflammatory responses or blood pressure issues. Understanding these relationships can inform therapeutic strategies targeting the kallikrein-kinin system for improved disease management.

产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

靶点信息

Glandular kallikreins cleave Met-Lys and Arg-Ser bonds in kininogen to release Lys-bradykinin.
See full target information Klk1

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