Anti-TIPRL抗体(ab70795)
Key features and details
- Rabbit polyclonal to TIPRL
- Suitable for: IHC-P, WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
概述
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产品名称
Anti-TIPRL抗体 -
描述
兔多克隆抗体to TIPRL -
宿主
Rabbit -
经测试应用
适用于: IHC-P, WB, IPmore details -
种属反应性
与反应: Mouse, Human
预测可用于: Chimpanzee, Rhesus monkey, Orangutan
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免疫原
Synthetic peptide corresponding to a region between residue 225 and the C-terminus (residue 272) of human TIPRL (NP_690866.1)
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阳性对照
- HeLa, 293T and NIH3T3 whole cell lysates.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
ab70795 was affinity purified using an epitope specific to TIPRL immobilized on solid support. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab70795于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P |
1/100 - 1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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| WB |
1/2000 - 1/10000. Detects a band of approximately 32 kDa (predicted molecular weight: 32 kDa).
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| IP |
Use at 2-5 µg/mg of lysate.
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| 说明 |
|---|
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IHC-P
1/100 - 1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
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WB
1/2000 - 1/10000. Detects a band of approximately 32 kDa (predicted molecular weight: 32 kDa). |
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IP
Use at 2-5 µg/mg of lysate. |
靶标
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功能
May be a allosteric regulator of serine/threonine-protein phosphatase 2A (PP2A). Isoform 1 inhibits catalytic activity of the PP2A(D) core complex in vitro. The PP2A(C):TIPRL complex does not show phosphatase activity. May play a role in the regulation of ATM/ATR signaling pathway controlling DNA replication and repair. -
序列相似性
Belongs to the TIP41 family. -
翻译后修饰
Phosphorylated upon DNA damage, probably by ATM or ATR. -
细胞定位
Cytoplasm. - Information by UniProt
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数据库链接
- Entrez Gene: 261726 Human
- Entrez Gene: 226591 Mouse
- Omim: 611807 Human
- SwissProt: O75663 Human
- SwissProt: Q8BH58 Mouse
- Unigene: 209431 Human
- Unigene: 21520 Mouse
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别名
- 1810011K17Rik antibody
- dJ69E11.3 antibody
- MGC3794 antibody
see all
图片
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Western blot - Anti-TIPRL antibody (ab70795)All lanes : Anti-TIPRL antibody (ab70795) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg/ml
Lane 2 : HeLa whole cell lysate at 15 µg/ml
Lane 3 : HeLa whole cell lysate at 5 µg/ml
Lane 4 : 293T whole cell lysate at 50 µg/ml
Lane 5 : NIH3T3 whole cell lysate at 50 µg/ml
Developed using the ECL technique.
Predicted band size: 32 kDa
Observed band size: 32 kDa
Exposure time: 3 seconds -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TIPRL antibody (ab70795)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma tissue labelling TIPRL with ab70795 at 1/200 (1µg/ml). Detection: DAB. -
Immunoprecipitation - Anti-TIPRL antibody (ab70795)Detection of TIPRL by Western Blot of Immunoprecipitate.
ab70795 at 1µg/ml staining TIPRL in HeLa whole cell lysate immunoprecipitated using ab70795 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection: Chemiluminescence with exposure time of 3 seconds.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (6)
ab70795 被引用在 6 文献中.
- Yen TL et al. Sex bias in social deficits, neural circuits and nutrient demand in Cttnbp2 autism models. Brain 146:2612-2626 (2023). PubMed: 36385662
- Xu X et al. Knockdown of TOR signaling pathway regulator suppresses cell migration and invasion in non-small cell lung cancer via the regulation of epithelial-to-mesenchymal transition. Exp Ther Med 19:1925-1932 (2020). PubMed: 32104250
- Luan M et al. TIPRL, a Novel Tumor Suppressor, Suppresses Cell Migration, and Invasion Through Regulating AMPK/mTOR Signaling Pathway in Gastric Cancer. Front Oncol 10:1062 (2020). PubMed: 32719745
- Huang L et al. NUP37 silencing induces inhibition of cell proliferation, G1 phase cell cycle arrest and apoptosis in non-small cell lung cancer cells. Pathol Res Pract 216:152836 (2020). PubMed: 32014308
- Shen H et al. YRDC is upregulated in non-small cell lung cancer and promotes cell proliferation by decreasing cell apoptosis. Oncol Lett 20:43-52 (2020). PubMed: 32565932
- Tirosh O et al. The Transcription and Translation Landscapes during Human Cytomegalovirus Infection Reveal Novel Host-Pathogen Interactions. PLoS Pathog 11:e1005288 (2015). WB . PubMed: 26599541