重组Anti-TIP49A抗体[EPR6028] (ab133513)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR6028] to TIP49A
- Suitable for: Flow Cyt (Intra), WB
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-TIP49A抗体[EPR6028]
参阅全部 TIP49A 一抗 -
描述
兔单克隆抗体[EPR6028] to TIP49A -
宿主
Rabbit -
经测试应用
适用于: Flow Cyt (Intra), WBmore details
不适用于: ICC/IF,IHC-P or IP -
种属反应性
与反应: Human
预测可用于: Mouse, Rat -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- K562, HT1080 and HT29 cell lysates. 293T cells.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
解离常数(KD)
KD = 1.95 x 10 -10 M Learn more about KD -
存储溶液
pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
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纯度
Tissue culture supernatant -
克隆
单克隆 -
克隆编号
EPR6028 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab133513于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
Flow Cyt (Intra) |
1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
WB |
1/1000 - 1/10000. Predicted molecular weight: 50 kDa.
|
说明 |
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Flow Cyt (Intra)
1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
WB
1/1000 - 1/10000. Predicted molecular weight: 50 kDa. |
靶标
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功能
Possesses single-stranded DNA-stimulated ATPase and ATP-dependent DNA helicase (3' to 5') activity. Component of the NuA4 histone acetyltransferase complex which is involved in transcriptional activation of select genes principally by acetylation of nucleosomal histones H4 and H2A. This modification may both alter nucleosome - DNA interactions and promote interaction of the modified histones with other proteins which positively regulate transcription. This complex may be required for the activation of transcriptional programs associated with oncogene and proto-oncogene mediated growth induction, tumor suppressor mediated growth arrest and replicative senescence, apoptosis, and DNA repair. The NuA4 complex ATPase and helicase activities seem to be, at least in part, contributed by the association of RUVBL1 and RUVBL2 with EP400. NuA4 may also play a direct role in DNA repair when recruited to sites of DNA damage. RUVBL1 plays an essential role in oncogenic transformation by MYC and also modulates transcriptional activation by the LEF1/TCF1-CTNNB1 complex.
May be able to bind plasminogen at cell surface and enhance plasminogen activation.
Essential for cell proliferation. -
组织特异性
Ubiquitously expressed with high expression in heart, skeletal muscle and testis. -
序列相似性
Belongs to the ruvB family. -
结构域
Binding to MYC is dependent on a Myc domain essential for oncogenic activity. -
细胞定位
Nucleus matrix. Nucleus > nucleoplasm. Cytoplasm. Membrane. Cytoplasm > cytoskeleton > centrosome. Mainly localized in the nucleus, associated with nuclear matrix or in the nuclear cytosol, although it is also present in the cytoplasm and associated with the cell membranes. In prophase and prometaphase it is located at the centrosome and the branching microtubule spindles. After mitotic nuclear membrane disintigration it accumulates at the centrosome and sites of tubulin polymerization. As cells pass through metaphase and into telophase it is located close to the centrosome at the early phase of tubulin polymerization. In anaphase it accumulates at the zone of tubule interdigitation. In telophase it is found at polar tubule overlap, and it reappears at the site of chromosomal decondensation in the daughter cells. - Information by UniProt
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数据库链接
- Entrez Gene: 8607 Human
- Entrez Gene: 56505 Mouse
- Entrez Gene: 65137 Rat
- Omim: 603449 Human
- SwissProt: Q9Y265 Human
- SwissProt: P60122 Mouse
- SwissProt: P60123 Rat
- Unigene: 272822 Human
see all -
别名
- 49 kDa TATA box binding protein interacting protein antibody
- 49 kDa TATA box-binding protein-interacting protein antibody
- 49 kDa TBP interacting protein antibody
see all
图片
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All lanes : Anti-TIP49A antibody [EPR6028] (ab133513) at 1/1000 dilution
Lane 1 : K562 cell lysate
Lane 2 : 293T (Human embryonic kidney epithelial cell) cell lysate
Lane 3 : HT1080 cell lysate
Lane 4 : HT29 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat-anti-rabbit HRP at 1/2000 dilution
Developed using the ECL technique.
Predicted band size: 50 kDa -
Intracellular flow cytometric analysis of permeablized 293T cells labelling TIP49A with ab133513 at 1/10 dilution (red) or negative Rabbit IgG (green).
数据表及文件
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SDS download
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Datasheet download
文献 (3)
ab133513 被引用在 3 文献中.
- Shalini V et al. Genome-wide occupancy reveals the localization of H1T2 (H1fnt) to repeat regions and a subset of transcriptionally active chromatin domains in rat spermatids. Epigenetics Chromatin 14:3 (2021). PubMed: 33407810
- McClurg UL et al. Human ex vivo prostate tissue model system identifies ING3 as an oncoprotein. Br J Cancer 118:713-726 (2018). PubMed: 29381681
- O'Neill DJ et al. SETD6 controls the expression of estrogen-responsive genes and proliferation of breast carcinoma cells. Epigenetics 9:942-50 (2014). PubMed: 24751716