Anti-TIMP4抗体(ab58425)
Key features and details
- Rabbit polyclonal to TIMP4
- Suitable for: ELISA, IHC-Fr, IHC-P, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
概述
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产品名称
Anti-TIMP4抗体
参阅全部 TIMP4 一抗 -
描述
兔多克隆抗体to TIMP4 -
宿主
Rabbit -
经测试应用
适用于: ELISA, IHC-Fr, IHC-P, WBmore details -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Synthetic peptide derived from C terminal residues of human TIMP4
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Store at -20°C. Stable for 12 months at -20°C -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol, 0.87% Sodium chloride
Without Mg+2 and Ca+2 -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
ab58425 was affinity purified from rabbit antiserum by affinity chromatography using epitope specific immunogen. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab58425于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ELISA |
Use at an assay dependent concentration.
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| IHC-Fr |
Use at an assay dependent concentration. PubMed: 23525608
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| IHC-P |
Use at an assay dependent concentration.
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| WB |
1/500 - 1/1000. Detects a band of approximately 26 kDa (predicted molecular weight: 26 kDa).
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| 说明 |
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ELISA
Use at an assay dependent concentration. |
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IHC-Fr
Use at an assay dependent concentration. PubMed: 23525608 |
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IHC-P
Use at an assay dependent concentration. |
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WB
1/500 - 1/1000. Detects a band of approximately 26 kDa (predicted molecular weight: 26 kDa). |
靶标
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功能
Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor. Known to act on MMP-1, MMP-2, MMP-3, MMP-7 and MMP-9. -
组织特异性
Abundant in heart and present at low levels in many other tissues. -
序列相似性
Belongs to the protease inhibitor I35 (TIMP) family.
Contains 1 NTR domain. -
细胞定位
Secreted. - Information by UniProt
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数据库链接
- Entrez Gene: 7079 Human
- Entrez Gene: 110595 Mouse
- Entrez Gene: 680130 Rat
- Omim: 601915 Human
- SwissProt: Q99727 Human
- SwissProt: Q9JHB3 Mouse
- SwissProt: P81556 Rat
- Unigene: 591665 Human
see all -
别名
- Metalloproteinase inhibitor 4 antibody
- TIMP 4 antibody
- TIMP metallopeptidase inhibitor 4 antibody
see all
图片
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Western blot - Anti-TIMP4 antibody (ab58425)All lanes : Anti-TIMP4 antibody (ab58425) at 1/500 dilution
Lane 1 : NIH3T3 cell extract
Lane 2 : NIH3T3 cell extract with immunising peptide
Predicted band size: 26 kDa
Observed band size: 26 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TIMP4 antibody (ab58425)ab58425, at 1/50 dilution, staining TIMP4 in paraffin embedded human lung carcinoma tissue by Immunohistochemistry, in the absence (left image) or presence (right image) of the immunising peptide.
数据表及文件
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SDS download
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Datasheet download
文献 (13)
ab58425 被引用在 13 文献中.
- Chan BYH et al. MMP inhibitors attenuate doxorubicin cardiotoxicity by preventing intracellular and extracellular matrix remodelling. Cardiovasc Res 117:188-200 (2021). PubMed: 31995179
- Dahal S et al. Quantitative Morphometry of Elastic Fibers in Pelvic Organ Prolapse. Ann Biomed Eng 49:1909-1922 (2021). PubMed: 33768411
- Jameson SA et al. Elastin homeostasis is altered with pelvic organ prolapse in cultures of vaginal cells from a lysyl oxidase-like 1 knockout mouse model. Physiol Rep 8:e14436 (2020). PubMed: 32533648
- Jana S et al. Disparate Remodeling of the Extracellular Matrix and Proteoglycans in Failing Pediatric Versus Adult Hearts. J Am Heart Assoc 7:e010427 (2018). PubMed: 30371322
- El Hajj EC et al. Inhibitor of lysyl oxidase improves cardiac function and the collagen/MMP profile in response to volume overload. Am J Physiol Heart Circ Physiol 315:H463-H473 (2018). PubMed: 29775412