Anti-TIMP4抗体(ab38987)
Key features and details
- Rabbit polyclonal to TIMP4
- Suitable for: WB
- Reacts with: Rat, Human
- Isotype: IgG
概述
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产品名称
Anti-TIMP4抗体
参阅全部 TIMP4 一抗 -
描述
兔多克隆抗体to TIMP4 -
宿主
Rabbit -
特异性
Ab38987 recognises the carboxiterminal region of TIMP4. It does not react with other TIMPs. -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Rat, Human -
免疫原
Synthetic peptide based on the carboxyterminal region of human TIMP4.
参阅Abcam的专有抗源政策 (Peptide available as ab41155.) -
阳性对照
- Human TIMP4, cell media from human colorectal adenosarcoma and rat heart.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
存储溶液
pH: 7.40
Preservative: 0.05% Sodium azide
Constituent: 2.9% Sodium chloride -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab38987于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (1) |
1/1000 - 1/5000. Detects a band of approximately 23 kDa (predicted molecular weight: 26 kDa).
Recommended starting dilution of 1/1000 when using colorimetric substrates such as BCIP/NBT and 1/5000 when using chemiluminescent substrates. Predicted molecular weight: 26 kDa. When used against the reduced protein, ab38987 identifies bands at 23 and 29 kDa, representing the unglycosylated and glycosylated forms of TIMP4 respectively. Treatment of cells with the phorbol ester TPA stimulates production of TIMP4 in some cell types but the low protein levels produced often require concentration of cell culture media to visualize the bands by Western Blotting. Dilution optimised using Chromogenic detection. |
| 说明 |
|---|
|
WB
1/1000 - 1/5000. Detects a band of approximately 23 kDa (predicted molecular weight: 26 kDa). Recommended starting dilution of 1/1000 when using colorimetric substrates such as BCIP/NBT and 1/5000 when using chemiluminescent substrates. Predicted molecular weight: 26 kDa. When used against the reduced protein, ab38987 identifies bands at 23 and 29 kDa, representing the unglycosylated and glycosylated forms of TIMP4 respectively. Treatment of cells with the phorbol ester TPA stimulates production of TIMP4 in some cell types but the low protein levels produced often require concentration of cell culture media to visualize the bands by Western Blotting. Dilution optimised using Chromogenic detection. |
靶标
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功能
Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor. Known to act on MMP-1, MMP-2, MMP-3, MMP-7 and MMP-9. -
组织特异性
Abundant in heart and present at low levels in many other tissues. -
序列相似性
Belongs to the protease inhibitor I35 (TIMP) family.
Contains 1 NTR domain. -
细胞定位
Secreted. - Information by UniProt
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数据库链接
- Entrez Gene: 7079 Human
- Entrez Gene: 680130 Rat
- Omim: 601915 Human
- SwissProt: Q99727 Human
- SwissProt: P81556 Rat
- Unigene: 591665 Human
- Unigene: 155651 Rat
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别名
- Metalloproteinase inhibitor 4 antibody
- TIMP 4 antibody
- TIMP metallopeptidase inhibitor 4 antibody
see all
图片
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Western blot - Anti-TIMP4 antibody (ab38987)All lanes : Anti-TIMP4 antibody (ab38987) at 1/1000 dilution
Lane 1 : human TIMP4
Lane 2 : cell media from human colorectal adenosarcoma
Lane 3 : rat heart
Predicted band size: 26 kDa
Observed band size: 25,45,66,97 kDa why is the actual band size different from the predicted?
Additional bands at: 20 kDa, 23 kDa (possible glycosylated form). We are unsure as to the identity of these extra bands.
数据表及文件
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Datasheet download
文献 (2)
ab38987 被引用在 2 文献中.
- Corporan D et al. Temporal changes in myocardial collagen, matrix metalloproteinases, and their tissue inhibitors in the left ventricular myocardium in experimental chronic mitral regurgitation in rodents. Am J Physiol Heart Circ Physiol 315:H1269-H1278 (2018). PubMed: 30141979
- Horn MA et al. Age-related divergent remodeling of the cardiac extracellular matrix in heart failure: collagen accumulation in the young and loss in the aged. J Mol Cell Cardiol 53:82-90 (2012). WB ; Sheep . PubMed: 22516365
