Anti-TIMP3抗体- Carboxyterminal end (ab39185)
Key features and details
- Rabbit polyclonal to TIMP3 - Carboxyterminal end
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-TIMP3抗体- Carboxyterminal end
参阅全部 TIMP3 一抗 -
描述
兔多克隆抗体to TIMP3 - Carboxyterminal end -
宿主
Rabbit -
特异性
This antibody recognizes both the glycosylated and unglycosylated forms of TIMP3, and works against native or reduced TIMP3. It does not cross react with the other TIMP family members (TIMP1, TIMP2, TIMP4). -
经测试应用
适用于: WB, ICC/IF, IHC-Pmore details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide based on the carboxyterminal region of Human TIMP3.
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阳性对照
- Human amd mouse TIMP3.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
存储溶液
Preservative: 0.05% Sodium azide
Constituent: 50% Glycerol -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab39185于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/1000 - 1/5000. Detects a band of approximately 21 kDa (predicted molecular weight: 24 kDa). Used under non reducing conditions.
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ICC/IF |
Use a concentration of 1 µg/ml.
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IHC-P | (1) |
Use at an assay dependent concentration.
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说明 |
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WB
1/1000 - 1/5000. Detects a band of approximately 21 kDa (predicted molecular weight: 24 kDa). Used under non reducing conditions. |
ICC/IF
Use a concentration of 1 µg/ml. |
IHC-P
Use at an assay dependent concentration. |
靶标
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功能
Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor. May form part of a tissue-specific acute response to remodeling stimuli. Known to act on MMP-1, MMP-2, MMP-3, MMP-7, MMP-9, MMP-13, MMP-14 and MMP-15. -
疾病相关
Defects in TIMP3 are the cause of Sorsby fundus dystrophy (SFD) [MIM:136900]. SFD is a rare autosomal dominant macular disorder with an age of onset in the fourth decade. It is characterized by loss of central vision from subretinal neovascularization and atrophy of the ocular tissues. Generally, macular disciform degeneration develops in the patients eye within 6 months to 6 years. -
序列相似性
Belongs to the protease inhibitor I35 (TIMP) family.
Contains 1 NTR domain. -
细胞定位
Secreted > extracellular space > extracellular matrix. - Information by UniProt
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数据库链接
- Entrez Gene: 7078 Human
- Omim: 188826 Human
- SwissProt: P35625 Human
- Unigene: 644633 Human
- Unigene: 714168 Human
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别名
- HSMRK222 antibody
- K222 antibody
- K222TA2 antibody
see all
图片
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Anti-TIMP3 antibody - Carboxyterminal end (ab39185) at 1 µg/ml + Lung (Human) Tissue Lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 24 kDa
Observed band size: 24 kDa
Additional bands at: 100 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 90 seconds -
ICC/IF image of ab39185 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab39185, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TIMP3 antibody - Carboxyterminal end (ab39185)Ab39185 staining Human normal placenta. Staining is localized to the cytoplasm or excreted.
Left panel: with primary antibody at 2 ug/ml. Right panel: isotype control.
Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffer citrate EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS), then incubated with primary antibody for 20 minutes, and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
数据表及文件
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SDS download
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Datasheet download
文献 (4)
ab39185 被引用在 4 文献中.
- Niu T et al. Long non-coding RNA RPSAP52 upregulates Timp3 by serving as the endogenous sponge of microRNA-365 in diabetic retinopathy. Exp Ther Med 20:246 (2020). PubMed: 33178344
- Pricola Fehnel K et al. Using urinary bFGF and TIMP3 levels to predict the presence of juvenile pilocytic astrocytoma and establish a distinct biomarker signature. J Neurosurg Pediatr 18:396-407 (2016). PubMed: 27314542
- Fiorentino L et al. Loss of TIMP3 underlies diabetic nephropathy via FoxO1/STAT1 interplay. EMBO Mol Med 5:441-55 (2013). Mouse . PubMed: 23401241
- Thomay AA et al. Disruption of interleukin-1 signaling improves the quality of wound healing. Am J Pathol 174:2129-36 (2009). WB ; Mouse . PubMed: 19389930