Anti-TIMP3抗体(ab39184)

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I have checked, but unfortunately, for these antibodies we do not have more immunogen information than what is on the datasheet. The source where we receive these 3 antibodies from, considers any immunogen information proprietary and therefore we do not have this information ourselves.
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Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement with ab39184.

To check the status of the order please contact our Customer Service team and reference this number.

Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.

I wish you the best of luck with your research.

Thank you for contacting Abcam. I can confirm there is 250mM sodium chloride in the storage buffer for ab39184. Please let me know if there is anything else I can help you with.

Thank you for taking time to complete our questionnaire. I am sorry to hear that antibodies ab16123, ab39184 and ab61224 are not providing satisfactory results. The details provided will enable us to investigate these cases and will provide us with vital information for monitoring product quality. Having reviewed these 3 cases, I would like to offer some suggestions to help optimize the results from ab16123, ab39184 and ab61224 : To reduce the background, I recommend to load a maximum of 20µg of samples on the gel. I would suggest to use each primary antibody at a dilution 1/1000 and to dilute each one in the blocking solution. The dilution factor can then be optimized around this value. I would also recommend to try an incubation at room temperature for 2 or 3 hours. Some antibodies bind stonger and more specifically at room temperature. In order to determine the origin of the background I would recommend to run a no primary control in order to see if the background is due to some non-specific binding by the secondary antibody. Last but not least, when testing our antibodies, our lab uses 5% BSA as a blocking reagent, so I recommend switching to this instead of milk. Some antibodies give stronger, more specific signals on blots blocked with BSA instead of milk, so doing this may improve the results you are seeing, and reduce the non-specific bands. An example of the above is the western blot obtained with the Abcam GAPDH antibody ab9385 : www.abcam.com/gapdh-antibody-hrp-loading-control-ab9385.html#GAPDH-Primary-antibodies-ab9385-3.jpg . Should the suggestions not improve the results, please do let me know. In the event that a product is not functioning in the species and applications cited on the product datasheet (and the problem has been reported within 6 months of purchase), we would be pleased to provide a free of charge replacement, credit note, or refund. I hope this information is helpful, and I thank you for your cooperation.

Thank you for taking the time to contact us. I am sorry to hear you have had difficulty obtaining satisfactory results from these antibodies. The details you have kindly provided will enable us to investigate this case for you and also gives us vital information for our monitoring of product quality. 1) When testing our antibodies, our lab uses 5% BSA as a blocking reagent, so I recommend switching to this instead of milk. For unknown reasons some antibodies give stronger, more specific signals on blots blocked with BSA instead of milk, so doing this may improve the results you are seeing, and reduce the non-specific bands. An example of the above is the western blot obtained with the Abcam GAPDH antibody ab9385 : Click here for the western blot image using ab9385 (or use the following: www.abcam.com/ab9385).   2) I am unsure if the bands you are observing are around 80 kDa, as the resolution of the higher molecular weight marker bands is suboptimal. In case no bands have run out of the gel and you count from the bottom, the TIMP3 bands could also be around 60 kDa. If that is the case they may represent multimers of the protein (32 kDa dimers were reported according to the literature) or complexes with metalloproteinases. Therefore, I would recommend boiling the samples for more than 5 minutes (e.g. 10min) which may help to reduce disulfide bridges and break up complex structures. We are happy to offer this technical support. In the event that the product is not functioning in the applications cited on the product data sheet (and the problem has been reported within 6 months of purchase), we will be pleased to provide a credit note, free of charge replacement or refund. I hope this information is helpful, thank you for your cooperation. Should the suggestions not improve the results, please do not hesitate to contact me again with the further requested details.  

Thank you for contacting us. We have indeed in our catalog 5 unconjugated anti-interferon alpha 1 antibodies tested and guaranteed to work in Western Blot and Human samples. Please note that, unfortunately, import restrictions make the goat polyclonal antibody ab86316 unavailable to Canadian customers. The remaining 4 products are very similar. However, I would recommend : - the anti-Interferon alpha antibody clone [AE3] reference ab10077 (www.abcam.com/ab10077) or - the anti-Interferon alpha antibody clone [C10F5] reference ab8317 (www.abcam.com/ab8317). These two mouse monoclonal antibodies, protein A purified, are currently in stock, and their unit size is 250µg which makes them cheaper than the other anti-IFNA1. About the other targets, I agree with you, I would recommend - ab39184 for the detection of TIMP3, - ab61224 for TIMP1, - ab95451 (Mouse monoclonal) for Maspin. ab22354 (Rabbit polyclonal) is also a good choice, - ab16123 for CDKN2A/p16INK4a. ab54210 is also well characterised with 2 publications and 3 Abreviews (customer feedbacks). I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.