Anti-TIMP2抗体(ab53730)
Key features and details
- Rabbit polyclonal to TIMP2
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-TIMP2抗体
参阅全部 TIMP2 一抗 -
描述
兔多克隆抗体to TIMP2 -
宿主
Rabbit -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide derived from human TIMP2.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, PBS
Without Mg+2 and Ca+2 -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
ab53730 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab53730于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/500 - 1/1000. Detects a band of approximately 31 kDa (predicted molecular weight: 24 kDa).
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| 说明 |
|---|
|
WB
1/500 - 1/1000. Detects a band of approximately 31 kDa (predicted molecular weight: 24 kDa). |
靶标
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功能
Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor. Known to act on MMP-1, MMP-2, MMP-3, MMP-7, MMP-8, MMP-9, MMP-10, MMP-13, MMP-14, MMP-15, MMP-16 and MMP-19. -
序列相似性
Belongs to the protease inhibitor I35 (TIMP) family.
Contains 1 NTR domain. -
翻译后修饰
The activity of TIMP2 is dependent on the presence of disulfide bonds. -
细胞定位
Secreted. - Information by UniProt
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数据库链接
- Entrez Gene: 7077 Human
- Omim: 188825 Human
- SwissProt: P16035 Human
- Unigene: 633514 Human
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别名
- CSC 21K antibody
- CSC-21K antibody
- CSC21K antibody
see all
图片
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Western blot - Anti-TIMP2 antibody (ab53730)All lanes : Anti-TIMP2 antibody (ab53730) at 1/500 dilution
Lane 1 : A549 cell extract
Lane 2 : A549 cell extract with immunising peptide
Predicted band size: 24 kDa
Observed band size: 31 kDa why is the actual band size different from the predicted?
数据表及文件
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SDS download
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Datasheet download
文献 (6)
ab53730 被引用在 6 文献中.
- Hu C et al. p38/JNK Is Required for the Proliferation and Phenotype Changes of Vascular Smooth Muscle Cells Induced by L3MBTL4 in Essential Hypertension. Int J Hypertens 2020:3123968 (2020). PubMed: 33381308
- Blache U et al. Mesenchymal stromal cell activation by breast cancer secretomes in bioengineered 3D microenvironments. Life Sci Alliance 2:N/A (2019). PubMed: 31160380
- Hussain H et al. A protein chimera strategy supports production of a model "difficult-to-express" recombinant target. FEBS Lett 592:2499-2511 (2018). PubMed: 29933498
- Sharif R et al. Collagen cross-linking impact on keratoconus extracellular matrix. PLoS One 13:e0200704 (2018). PubMed: 30020979
- Smith AC et al. Synaptic plasticity mediating cocaine relapse requires matrix metalloproteinases. Nat Neurosci 17:1655-7 (2014). IHC ; Mouse . PubMed: 25326689
- Fortin CF et al. MT6-MMP is present in lipid rafts and faces inward in living human PMNs but translocates to the cell surface during neutrophil apoptosis. Int Immunol : (2010). PubMed: 20501611