为了使您在Abcam官网的浏览体验更顺畅,请使用最新版本的浏览器比如 Google Chrome

Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
致电 +86 21 2070 0500 或 联系我们

  • 我的账户
  • 退出
登录 或 注册

欢迎光临

登录 或

您还没有帐户?

注册
我的购物篮
快速订购
Abcam homepage

  • 研究用途的产品
    按产品类型
    一抗
    二抗
    免疫测定试剂盒与试剂
    细胞与组织成像工具
    细胞与生化测定
    蛋白质与肽
    按产品类型
    蛋白质组学工具
    激动剂、活化剂、拮抗剂与抑制剂
    细胞株和裂解液
    多重miRNA检测
    多因子分析试剂盒
    按研究领域
    癌症
    心血管
    细胞生物学
    表观遗传学
    代谢
    发育生物学
    按研究领域
    免疫学
    微生物学
    神经科学
    信号转导
    干细胞
  • 定制化产品 & 合作关系
    定制化产品 & 合作关系

    定制化产品和商务合作加速您的诊断和治疗项目

    定制化产品

    与我们成为伙伴

  • 技术支持
    支持中心

    获取研究建议和支持

    查看支持中心

    Protocols

    一步一步完成您的实验

    查看Protocols

  • 活动通知
    • 会议年历
    • 肿瘤研究
    • 心血管研究
    • 染色质及细胞核信号转导研究
    • 免疫学
    • 神经生物学
    • 干细胞研究
    • 商品展览
    • 实验技术视频
    查看最新活动

    活动说明、摘要提交、演讲者、注册方式及更多信息

    查看全球活动安排

  • 通路
    细胞信号转导通路

    查看所有通路

    查看交叉通路

Cardiovascular Angiogenesis Adhesion / ECM Matrix Metalloproteinases TIMP

Anti-TIMP2抗体[3A4] (ab1828)

  • Datasheet
  • SDS
Reviews (7)Q&A (14)References (51)

Product price, shipping and contact information

Currently unavailable

抱歉,我们目前无法显示此内容。

请联系我们以完成下单,或者之后再尝试访问。

 

正在加载规格和价格。。。

 

送货和订单信息

运输费用

Promotion Information

Abpromise

产品质量保证,客户专家支持,

点击获取更多

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TIMP2 antibody [3A4] (ab1828)

    Key features and details

    • Mouse monoclonal [3A4] to TIMP2
    • Suitable for: IHC-P
    • Reacts with: Human
    • Isotype: IgG2a

    你可能会感兴趣

    二抗
    Product image
    Goat Anti-Mouse IgG H&L (HRP) (ab205719)
    分析检测
    Product image
    Annexin V-iFluor 488 Apoptosis Detection Kit (ab219916)
    一抗
    Product image
    Anti-SerpinB2/PAI-2 antibody (ab47742)

    查看更多相关产品

    概述

    • 产品名称

      Anti-TIMP2抗体[3A4]
      参阅全部 TIMP2 一抗
    • 描述

      小鼠单克隆抗体[3A4] to TIMP2
    • 宿主

      Mouse
    • 经测试应用

      适用于: IHC-Pmore details
    • 种属反应性

      与反应: Human
      预测可用于: Mouse, Rat, Rabbit, Guinea pig, Cow
    • 免疫原

      Synthetic peptide corresponding to Human TIMP2 (N terminal).

    • 阳性对照

      • IHC-P: Normal human placenta tissue.
    • 常规说明

      This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    性能

    • 形式

      Liquid
    • 存放说明

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
    • 存储溶液

      Preservative: 0.02% Sodium azide
      Constituent: PBS
    • Concentration information loading...
    • 纯度

      Protein G purified
    • 克隆

      单克隆
    • 克隆编号

      3A4
    • 同种型

      IgG2a
    • 轻链类型

      kappa
    • 研究领域

      • Cardiovascular
      • Angiogenesis
      • Adhesion / ECM
      • Matrix Metalloproteinases
      • TIMP
      • Signal Transduction
      • Cytoskeleton / ECM
      • Extracellular Matrix
      • ECM Enzymes
      • MMP Inhibitors
      • Cancer
      • Invasion/microenvironment
      • Angiogenesis
      • ECM enzymes
      • TIMPs
      • Cancer
      • Invasion/microenvironment
      • ECM
      • Extracellular matrix
      • TIMPs
      • Cell Biology
      • Proteolysis / Ubiquitin
      • Protease inhibitors
      • Metalloprotease inhibitors
      • TIMPs
      • Cardiovascular
      • Atherosclerosis
      • Thrombosis
      • Endothelial mediators/regulators

    相关产品

    • Compatible Secondaries

      • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
      • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
    • Isotype control

      • Mouse IgG2a, kappa monoclonal [MG2a-53] - Isotype control (ab18415)

    应用

    The Abpromise guarantee

    Abpromise™承诺保证使用ab1828于以下的经测试应用

    “应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

    应用 Ab评论 说明
    IHC-P (1)
    Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
    说明
    IHC-P
    Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    靶标

    • 功能

      Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor. Known to act on MMP-1, MMP-2, MMP-3, MMP-7, MMP-8, MMP-9, MMP-10, MMP-13, MMP-14, MMP-15, MMP-16 and MMP-19.
    • 序列相似性

      Belongs to the protease inhibitor I35 (TIMP) family.
      Contains 1 NTR domain.
    • 翻译后修饰

      The activity of TIMP2 is dependent on the presence of disulfide bonds.
    • 细胞定位

      Secreted.
    • Target information above from: UniProt accession P16035 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • 数据库链接

      • Entrez Gene: 282093 Cow
      • Entrez Gene: 100135629 Guinea pig
      • Entrez Gene: 7077 Human
      • Entrez Gene: 21858 Mouse
      • Entrez Gene: 100008689 Rabbit
      • Entrez Gene: 29543 Rat
      • Omim: 188825 Human
      • SwissProt: P16368 Cow
      • SwissProt: Q9WUC6 Guinea pig
      • SwissProt: P16035 Human
      • SwissProt: P25785 Mouse
      • SwissProt: Q9TRZ7 Rabbit
      • SwissProt: P30121 Rat
      • Unigene: 633514 Human
      • Unigene: 206505 Mouse
      • Unigene: 10161 Rat
      see all
    • 别名

      • CSC 21K antibody
      • CSC-21K antibody
      • CSC21K antibody
      • Metalloproteinase inhibitor 2 antibody
      • Metalloproteinase inhibitor 2 precursor antibody
      • TIMP 2 antibody
      • TIMP metallopeptidase inhibitor 2 antibody
      • TIMP-2 antibody
      • TIMP2 antibody
      • TIMP2_HUMAN antibody
      • Tissue Inhibitor of Metalloproteinase 2 antibody
      • Tissue inhibitor of metalloproteinases 2 antibody
      see all

    图片

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TIMP2 antibody [3A4] (ab1828)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TIMP2 antibody [3A4] (ab1828)

      IHC image of TIMP2 staining in a section of formalin-fixed paraffin-embedded normal human placenta* performed on a Leica BONDTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab1828, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.

      For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

      *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

    实验方案

    • Immunohistochemistry protocols

    Click here to view the general protocols

    数据表及文件

    • SDS download

    • Datasheet download

      Download

    文献 (51)

    发表研究结果有使用 ab1828?请让我们知道,以便我们可以引用本数据表中的参考文章。

    ab1828 被引用在 51 文献中.

    • Cui L  et al. Chaihu Guizhi Ganjiang Decoction Ameliorates Pancreatic Fibrosis via JNK/mTOR Signaling Pathway. Front Pharmacol 12:679557 (2021). PubMed: 34177589
    • Li S  et al. MicroRNA-4500 Inhibits Migration, Invasion, and Angiogenesis of Breast Cancer Cells via RRM2-Dependent MAPK Signaling Pathway. Mol Ther Nucleic Acids 21:278-289 (2020). PubMed: 32615527
    • Teixeira M  et al. Regular Exercise Participation Contributes to Better Proteostasis, Inflammatory Profile, and Vasoactive Profile in Patients With Hypertension. Am J Hypertens 33:119-123 (2020). PubMed: 31560738
    • Wesslowski J  et al. eGFP-tagged Wnt-3a enables functional analysis of Wnt trafficking and signaling and kinetic assessment of Wnt binding to full-length Frizzled. J Biol Chem 295:8759-8774 (2020). PubMed: 32381507
    • Eckert AF  et al. Measuring ligand-cell surface receptor affinities with axial line-scanning fluorescence correlation spectroscopy. Elife 9:N/A (2020). PubMed: 32441251
    View all Publications for this product

    客户评价及客户问答

    Show All 评价 Q&A
    提交评价 提交问题

    1-10 of 21 Abreviews or Q&A

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-TIMP2 antibody [3A4]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citrate
    Sample
    Human Tissue sections (Ascending aorta)
    Specification
    Ascending aorta
    Permeabilization
    No
    Fixative
    Formaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Dr. Mengcheng Shen

    Verified customer

    提交于 Feb 05 2015

    Western blot abreview for Anti-TIMP2 antibody [3A4]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Rat Tissue lysate - whole (ovaries)
    Gel Running Conditions
    Reduced Denaturing (12%)
    Loading amount
    100 µg
    Specification
    ovaries
    Blocking step
    Milk as blocking agent for 18 hour(s) and 0 minute(s) · Concentration: 10%
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Sep 30 2013

    Western blot abreview for Anti-TIMP2 antibody [3A4]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (Astrocytes)
    Gel Running Conditions
    Reduced Denaturing (12%)
    Loading amount
    50 µg
    Specification
    Astrocytes
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Dec 03 2012

    Immunocytochemistry/ Immunofluorescence abreview for Anti-TIMP2 antibody [3A4]

    Good
    Abreviews
    Abreviews
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (HeLa)
    Permeabilization
    Yes - 0,1% Triton X
    Specification
    HeLa
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
    Fixative
    Methanol
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Nov 29 2012

    Western blot abreview for Anti-TIMP2 antibody [3A4]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Rat Tissue lysate - whole (Skeletal Muscle)
    Gel Running Conditions
    Reduced Denaturing (4-12%)
    Loading amount
    50 µg
    Specification
    Skeletal Muscle
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Dr. Steve John

    Verified customer

    提交于 Aug 07 2012

    Question

    The customer want to test the below antibodies for Bovine & WB .

    None of the same is tested for the Bovine . So if it is feasible , then please issue discount code .

    1. Ab1828

    2. Ab8448

    3. Ab23706

    4. Ab49341

    5. Ab50693

    6. Ab101892

    7. Ab101894

    8. Ab125362

    Regards

    Read More

    Abcam community

    Verified customer

    Asked on Dec 27 2012

    Answer

    Thank you for contacting us.

    The antibodies ab1828, ab23706 and ab50693 are tested with Bovine (Cow) tissue and are fully guaranteed so the Abreview discount is not valid for these.

    I am happy to provide discount code for others antibodies however I will send 2 discount codes for a start, once customer gets good results then I will provide the codes for others. Could you please ask customer choosing the 2 antibodies from ab8448, ab49341, ab101892, ab101894 and ab125362.

    Could you also provide customer's email and institution name?

    Many thanks for your cooperation. I will look forward to hearing from you soon.

    Read More

    Abcam Scientific Support

    回复于 Dec 27 2012

    Question

    Inquiry: I'm looking for a positive control for ab1828 (anti-Timp2) to use for western blot. Will TIMP2 protein (mouse) ab39315 work as a positive control for ab1828 (anti-Timp2) in western blot?

    Read More

    Abcam community

    Verified customer

    Asked on Oct 01 2012

    Answer


    Yes you can use ab39315 as a positive control for western blotting with the anti-Timp2 antibody ab1828.
    There is a note on the online product datasheet for ab39315 with some further details, which states a western blot "Produces a band of approximately 22 kDa, corresponding to the non-glycolisated form. This protein can also be used as a positive control in substrate gel analysis (reverse zymograms)."
    .

    Read More

    Abcam Scientific Support

    回复于 Oct 01 2012

    Question

    Hi, I’m trying to find antibodies to detect canine TIMP-2 and MMP-14 and was wondering whether the products I list below are predicted to cross-react with the canine protein. I’ve attached alignments which I hope will be of some help to determine cross-reactivity. Anti-TIMP2 antibody [3A4] (ab1828) Anti-MMP14 antibody [113-5B7] (ab58815) Anti-MMP14 antibody - Hinge region (ab38971) Kind regards /

    Read More

    Abcam community

    Verified customer

    Asked on Dec 12 2011

    Answer

    Thank you for contacting us. According to the alignment information that you have sent to me it looks like you should have success with using these antibodies with canine protein. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.  

    Read More

    Abcam Scientific Support

    回复于 Dec 12 2011

    Question

    A replacement would be great and then I will likely order a large amount in the future.  Do I need to place an order or will you place the replacement order? Thanks so much for your help!  

    Read More

    Abcam community

    Verified customer

    Asked on Dec 09 2011

    Answer

    Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products. I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement. To check the status of the order please contact our Customer Service team and reference this number. Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know. I wish you the best of luck with your research.

    Read More

    Abcam Scientific Support

    回复于 Dec 09 2011

    Question

    I tried both TIMP antibodies at 1:1000, 1:500, and 1:100. I only got them to work at 1:100 with an overnight exposure. The control antibody I used that detects viral protein was easily detectable. I didn't quantify the amount of protein that I loaded, but I loaded the maximum 15ul volume. I included other samples on the same gel prepared the same way and they were easily detectable via WB using other antibodies. Block: 5% milk, .1% Tween-20 in PBS Wash: 1% milk, .1% Tween-20 in PBS Blocking and AB exposure 1. Trim and mark ladder 2. Wash the membrane in PBS 3. Block 2x 40' RT 4. Wash 2x 5 min in wash 5. Incubate in primary AB diluted in wash O/N 4 degrees 6. Wash 4x 10min in wash 7. Incubate in secondary AB diluted in wash 1 hr RT 8. Wash 4x 10 min in wash 9. Place in PBS 10. Develop I also tried ICC on transfected cells where both antibodies only worked at a 1:10 dilution with the following protocol: 1. Wash in PBS 2. Fix in 4% PFA in PBS 15' RT 3. Wash 3 times in PBS 4. Incubate in blocking solution for 15 min at RT 5. Wash with perm sol. 6. Incubate in perm sol. 15 min at RT 7. Incubate in primary antibody (dilute in perm sol) for 1.5hrs at RT 8. 3x 5 min washes in perm sol. 9. Incubate in secondary antibody for 1.5hrs at RT (dilute in perm. sol.). Cover with foil to protect from light from this point on. 10. 3x 5 min washes in perm sol. 1 1. Incubate in DAPI 1:10,000 in PBS for 10 min at RT 12. Wash with PBS 13. Add vectashield and mount Blocking solution PBS with 3% BSA and .01% sodium azide Permeablization solution PBS with 1% BSA, .1% TritonX-100, .01% sodium azide

    Read More

    Abcam community

    Verified customer

    Asked on Dec 08 2011

    Answer

    Thank you for taking the time to send me your protocols relating to this issue. I have been able to review them and agree that the problem here is the product. I would like to offer you free of charge replacements for ab1828 and ab61224. I may also offer you a credit note/refund that may be used against this or any outstanding invoices. As you have mentioned before that you will be using alot of this antibody for future experiments, I thought I might inform you that Abcam will discount orders above 5 units. Please let me know how you would like to procceed and do not hesitate to contact us with further questions or if there are other ways in which we might help you reach your research goals.  

    Read More

    Abcam Scientific Support

    回复于 Dec 08 2011

    1-10 of 21 Abreviews or Q&A

    •  向后
    • 1
    • 2
    • 3
    • 向前 

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    通过邮件收到学术资源和促销信息 注册
    研究类别(A-Z)
    • 肿瘤研究
    • 心血管研究
    • 细胞生物学
    • 发育生物学
    • 染色质及细胞核信号转导研究
    • 免疫学
    • 代谢
    • 微生物学
    • 神经生物学
    • 信号转导研究
    • 干细胞研究
    产品种类(A-Z)
    • 一抗
    • 二抗
    • 生化试剂
    • 亚类对照
    • 流式细胞仪多色选择器
    • 试剂盒
    • 内参对照
    • 细胞裂解液
    • 多肽
    • 蛋白酶
    • 组织切片
    • 蛋白标签及细胞标记物
    • 相关试剂及工具
    帮助与支持
    • 技术支持
    • 技术FAQ
    • 实验步骤与建议
    • 购买FAQ
    • RabMAb 产品
    • 培训课程
    • 按靶标浏览
    企业
    • 企业资讯
    • 投资者关系
    • 公司新闻
    • 招贤纳士
    • 关于我们
    活动通知
    • 商品展览
    • 会议
    全球网站
    • abcam.com
    • abcam.co.jp
    QR code for Weechat

    微信扫码 关注我们

    沪ICP备14046373号-1

    沪公网安备 31011502007413号

    • 销售条款
    • 网站使用条款
    • Cookie政策
    • 隐私权政策
    • 法律
    © 1998-2023 艾博抗(上海)贸易有限公司版权所有