重组Anti-TIMP1抗体[EPR16616] (ab179580)
![Western blot - Anti-TIMP1 antibody [EPR16616] (ab179580)](https://www.abcam.cn/ps/products/179/ab179580/Images/ab179580-304301-anti-timp1-antibody-epr16616-western-blot.jpg "Western blot - Anti-TIMP1 antibody [EPR16616] (ab179580)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR16616] to TIMP1
- Suitable for: WB
- Reacts with: Mouse
Related conjugates and formulations
概述
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产品名称
Anti-TIMP1抗体[EPR16616]
参阅全部 TIMP1 一抗 -
描述
兔单克隆抗体[EPR16616] to TIMP1 -
宿主
Rabbit -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Mouse -
免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: Mouse lung culture supernatant (7 days) and leg bone lysate.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR16616 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab179580于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/1000. Predicted molecular weight: 23 kDa.
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| 说明 |
|---|
|
WB
1/1000. Predicted molecular weight: 23 kDa. |
靶标
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功能
Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor. Also mediates erythropoiesis in vitro; but, unlike IL-3, it is species-specific, stimulating the growth and differentiation of only human and murine erythroid progenitors. Known to act on MMP-1, MMP-2, MMP-3, MMP-7, MMP-8, MMP-9, MMP-10, MMP-11, MMP-12, MMP-13 and MMP-16. Does not act on MMP-14. -
序列相似性
Belongs to the protease inhibitor I35 (TIMP) family.
Contains 1 NTR domain. -
翻译后修饰
The activity of TIMP1 is dependent on the presence of disulfide bonds. -
细胞定位
Secreted. - Information by UniProt
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数据库链接
- Entrez Gene: 21857 Mouse
- SwissProt: P12032 Mouse
- Unigene: 8245 Mouse
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别名
- Clgi antibody
- Collagenase inhibitor antibody
- Collagenase inhibitor, Human antibody
see all
图片
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Western blot - Anti-TIMP1 antibody [EPR16616] (ab179580)All lanes : Anti-TIMP1 antibody [EPR16616] (ab179580) at 1/1000 dilution
Lane 1 : Mouse lung culture supernatant (7 days) at 20 µl
Lane 2 : Mouse leg bone lysate at 10 µg
Secondary
Lane 1 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Lane 2 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Developed using the ECL technique.
Predicted band size: 23 kDa
Observed band size: 28 kDa why is the actual band size different from the predicted?Blocking / Dilution: 5% NFDM/TBST
Exposure time: Lane 1: 32 seconds; Lane 2: 3 minutes
The molecular mass observed is consistent with the literature (PMID: 17202148).
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![Anti-TIMP1 antibody [EPR16616] (ab179580)](https://www.abcam.cn/ps/products/179/ab179580/Images/ab179580-2-benefits-of-recombinant-antibodies.png)
Anti-TIMP1 antibody [EPR16616] (ab179580)
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
Certificate of Compliance
文献 (7)
ab179580 被引用在 7 文献中.
- Liu X et al. Relationship between circadian rhythm-related genes and extracellular matrix: implications for sleep deprivation. Sleep Breath 28:697-705 (2024). PubMed: 37923872
- Chalise U et al. Harnessing the Plasma Proteome to Mirror Current and Predict Future Cardiac Remodeling After Myocardial Infarction. J Cardiovasc Transl Res 16:3-16 (2023). PubMed: 36197585
- Kim Y et al. HL3501, a Novel Selective A3 Adenosine Receptor Antagonist, Lowers Intraocular Pressure (IOP) in Animal Glaucoma Models. Transl Vis Sci Technol 11:30 (2022). PubMed: 35191964
- Kimura S et al. Histological characteristics of matrix metalloproteinase-9 and tissue inhibitor of metalloproteinases-1 in asthmatic murine model during A(H1N1)pdm09 infection. Pathol Int 72:506-518 (2022). PubMed: 36066006
- Song J et al. Inactivation of Interleukin-4 Receptor α Signaling in Myeloid Cells Protects Mice From Angiotensin II/High Salt-Induced Cardiovascular Dysfunction Through Suppression of Fibrotic Remodeling. J Am Heart Assoc 10:e017329 (2021). PubMed: 34132103
- Ikeda M et al. Excessive Hypoxia-Inducible Factor-1α Expression Induces Cardiac Rupture via p53-Dependent Apoptosis After Myocardial Infarction. J Am Heart Assoc 10:e020895 (2021). PubMed: 34472375
- Wang Y et al. Kaempferol promotes proliferation, migration and differentiation of MC3T3-E1 cells via up-regulation of microRNA-101. Artif Cells Nanomed Biotechnol 47:1050-1056 (2019). PubMed: 30942633