重组Anti-Timeless抗体[EPR5275] (ab109512)
![Western blot - Anti-Timeless antibody [EPR5275] (ab109512)](https://www.abcam.cn/ps/products/109/ab109512/Images/ab109512-467547-anti-timeless-antibody-epr5275-westernblot-human.jpg "Western blot - Anti-Timeless antibody [EPR5275] (ab109512)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5275] to Timeless
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-Timeless抗体[EPR5275]
参阅全部 Timeless 一抗 -
描述
兔单克隆抗体[EPR5275] to Timeless -
宿主
Rabbit -
经测试应用
适用于: ICC/IF, WB, IHC-Pmore details
不适用于: Flow Cyt or IP -
种属反应性
与反应: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- IHC-P: Human lung cancer tissue; ICC: HeLa cells. WB: HeLa, JAR and jurkat whole cell lysate.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 0.05% BSA, 40% Glycerol (glycerin, glycerine), 59% PBS -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR5275 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab109512于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF | (1) |
1/50.
|
| WB |
1/50000. Detects a band of approximately 150 kDa (predicted molecular weight: 139 kDa).
For unpurified use at 1/50000 - 1/200000. |
|
| IHC-P |
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
For unpurified use at 1/100 - 1/250. |
| 说明 |
|---|
|
ICC/IF
1/50. |
|
WB
1/50000. Detects a band of approximately 150 kDa (predicted molecular weight: 139 kDa). For unpurified use at 1/50000 - 1/200000. |
|
IHC-P
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. For unpurified use at 1/100 - 1/250. |
靶标
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功能
Required for normal progression of S-phase. Involved in the circadian rhythm autoregulatory loop. Negatively regulates CLOCK-NPAS2/BMAL1-induced transactivation of PER1 possibly via translocation of PER1 into the nucleus. Promotes TIPIN nuclear localiZation. Involved in cell survival after DNA damage or replication stress. May be specifically required for the ATR-CHK1 pathway in the replication checkpoint induced by hydroxyurea or ultraviolet light. May also play an important role in epithelial cell morphogenesis and formation of branching tubules. -
组织特异性
Expressed in all tissues examined including brain, heart, lung, liver, skeletal muscle, kidney, placenta, pancreas, spleen, thymus and testis. Highest levels of expression in placenta, pancreas, thymus and testis. -
序列相似性
Belongs to the timeless family. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 8914 Human
- Omim: 603887 Human
- SwissProt: Q9UNS1 Human
- Unigene: 118631 Human
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别名
- FLJ12640 antibody
- FLJ20714 antibody
- hTIM antibody
see all
图片
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Western blot - Anti-Timeless antibody [EPR5275] (ab109512)All lanes : Anti-Timeless antibody [EPR5275] (ab109512) at 1/50000 dilution (Purified)
Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate
Lane 2 : JAR (Human placenta choriocarcinoma epithelial cell) whole cell lysate
Lane 3 : Jurkat (Human T cell leukemia T lymphocyte) whole cell lysate
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 139 kDa -
![Immunocytochemistry/ Immunofluorescence - Anti-Timeless antibody [EPR5275] (ab109512)](https://www.abcam.cn/ps/products/109/ab109512/Images/ab109512-7-anti-timeless-antibody-epr5275-immunocytochemistry-hela-human.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Timeless antibody [EPR5275] (ab109512)Immunocytochemistry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling Timeless with purified ab109512 at 1/50 dilution (2.5 µg/mL). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/mL). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/mL) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Timeless antibody [EPR5275] (ab109512)](https://www.abcam.cn/ps/products/109/ab109512/Images/ab109512-6-anti-timeless-antibody-epr5275-immunohistochemistry-lung-cancer-human.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Timeless antibody [EPR5275] (ab109512)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung cancer tissue sections labeling Timeless with purified ab109512 at 1/500 dilution (0.25 µg/mL). Heat mediated antigen retrieval was performed using Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 1 (pH 6.0). Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) secondary antibody was used at 1/0 dilution. PBS instead of the primary antibody was used as the negative control.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
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![Anti-Timeless antibody [EPR5275] (ab109512)](https://www.abcam.cn/ps/products/109/ab109512/Images/ab109512-5-benefits-of-recombinant-antibodies.png)
Anti-Timeless antibody [EPR5275] (ab109512)
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (10)
ab109512 被引用在 10 文献中.
- Andrs M et al. Excessive reactive oxygen species induce transcription-dependent replication stress. Nat Commun 14:1791 (2023). PubMed: 36997515
- Shi D et al. Six-hour time-restricted feeding inhibits lung cancer progression and reshapes circadian metabolism. BMC Med 21:417 (2023). PubMed: 37924048
- Zhang G et al. A Novel Molecular Classification Method for Glioblastoma Based on Tumor Cell Differentiation Trajectories. Stem Cells Int 2023:2826815 (2023). PubMed: 37964983
- Yang Y et al. An integrative evaluation of circadian gene TIMELESS as a pan-cancer immunological and predictive biomarker. Eur J Med Res 28:563 (2023). PubMed: 38053143
- Polasek-Sedlackova H et al. Solving the MCM paradox by visualizing the scaffold of CMG helicase at active replisomes. Nat Commun 13:6090 (2022). PubMed: 36241664
- Zonderland G et al. The TRESLIN-MTBP complex couples completion of DNA replication with S/G2 transition. Mol Cell 82:3350-3365.e7 (2022). PubMed: 36049481
- Fan Y et al. LRR1-mediated replisome disassembly promotes DNA replication by recycling replisome components. J Cell Biol 220:N/A (2021). PubMed: 34037657
- Zhou J et al. Aberrantly Expressed Timeless Regulates Cell Proliferation and Cisplatin Efficacy in Cervical Cancer. Hum Gene Ther 31:385-395 (2020). PubMed: 31870179
- Bianco JN et al. Overexpression of Claspin and Timeless protects cancer cells from replication stress in a checkpoint-independent manner. Nat Commun 10:910 (2019). PubMed: 30796221
- Baldeyron C et al. TIPIN depletion leads to apoptosis in breast cancer cells. Mol Oncol 9:1580-98 (2015). PubMed: 26004086