重组Anti-TIM50抗体[EPR5784] (ab109527)
![Western blot - Anti-TIM50 antibody [EPR5784] (ab109527)](https://www.abcam.cn/ps/products/109/ab109527/Images/ab109527-122459-anti-tim50-antibody-epr5784-western-blot.jpg "Western blot - Anti-TIM50 antibody [EPR5784] (ab109527)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5784] to TIM50
- Suitable for: WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-TIM50抗体[EPR5784]
参阅全部 TIM50 一抗 -
描述
兔单克隆抗体[EPR5784] to TIM50 -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-Pmore details
不适用于: Flow Cyt,ICC/IF or IP -
种属反应性
与反应: Human
预测可用于: Mouse
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免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- 293T, SH SY5Y, HepG2 and HeLa cell lysates Human kidney tissue, human liver tissue (IHC-P)
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR5784 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab109527于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/10000 - 1/50000. Predicted molecular weight: 40 kDa.
|
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| IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
| 说明 |
|---|
|
WB
1/10000 - 1/50000. Predicted molecular weight: 40 kDa. |
|
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
应用说明
Is unsuitable for Flow Cyt,ICC/IF or IP.
靶标
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功能
Essential component of the TIM23 complex, a complex that mediates the translocation of transit peptide-containing proteins across the mitochondrial inner membrane. Has some phosphatase activity in vitro; however such activity may not be relevant in vivo. Isoform 2 may participate in the release of snRNPs and SMN from the Cajal body. -
组织特异性
Widely expressed. Expressed at higher level in brain, kidney and liver (at protein level). -
序列相似性
Belongs to the TIM50 family.
Contains 1 FCP1 homology domain. -
结构域
The FCP1 homology domain does not contain the canonical D-x-D-x-[TV] active site, suggesting that it probably does not display phosphatase activity in vivo. -
细胞定位
Mitochondrion inner membrane and Nucleus speckle. Nuclear and enriched in speckles with snRNPs. - Information by UniProt
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数据库链接
- Entrez Gene: 92609 Human
- Entrez Gene: 66525 Mouse
- Omim: 607381 Human
- SwissProt: Q3ZCQ8 Human
- SwissProt: Q9D880 Mouse
- Unigene: 590956 Human
- Unigene: 597106 Human
- Unigene: 167913 Mouse
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别名
- Homolog of yeast Tim50 antibody
- Import inner membrane translocase subunit TIM 50 antibody
- Import inner membrane translocase subunit TIM50 antibody
see all
图片
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Western blot - Anti-TIM50 antibody [EPR5784] (ab109527)All lanes : Anti-TIM50 antibody [EPR5784] (ab109527) at 1/10000 dilution
Lane 1 : 293T (Human embryonic kidney epithelial cell) cell lysate
Lane 2 : SH-SY5Y cell lysate
Lane 3 : HepG2 cell lysate
Lane 4 : HeLa cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 40 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TIM50 antibody [EPR5784] (ab109527)](https://www.abcam.cn/ps/products/109/ab109527/Images/ab109527-122458-anti-tim50-antibody-epr5784-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TIM50 antibody [EPR5784] (ab109527)ab109527, at 1/50, staining TIM50 in paraffin-embedded human kidney tissue by Immunohistochemistry.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TIM50 antibody [EPR5784] (ab109527)](https://www.abcam.cn/ps/products/109/ab109527/Images/ab109527-122457-anti-tim50-antibody-epr5784-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TIM50 antibody [EPR5784] (ab109527)ab109527, at 1/50, staining TIM50 in paraffin-embedded human liver tissue by Immunohistochemistry.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Anti-TIM50 antibody [EPR5784] (ab109527)](https://www.abcam.cn/ps/products/109/ab109527/Images/ab109527-4-benefits-of-recombinant-antibodies.png)
Anti-TIM50 antibody [EPR5784] (ab109527)
数据表及文件
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SDS download
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Datasheet download
文献 (4)
ab109527 被引用在 4 文献中.
- Wang X et al. Dihydroartemisinin synergistically enhances the cytotoxic effects of oxaliplatin in colon cancer by targeting the PHB2-RCHY1 mediated signaling pathway. Mol Carcinog 62:293-302 (2023). PubMed: 36342357
- Zhang X et al. TIMM50 promotes tumor progression via ERK signaling and predicts poor prognosis of non-small cell lung cancer patients. Mol Carcinog 58:767-776 (2019). PubMed: 30604908
- Burstein SR et al. In vitro and in vivo studies of the ALS-FTLD protein CHCHD10 reveal novel mitochondrial topology and protein interactions. Hum Mol Genet 27:160-177 (2018). PubMed: 29112723
- Lobo-Jarne T et al. Human COX7A2L Regulates Complex III Biogenesis and Promotes Supercomplex Organization Remodeling without Affecting Mitochondrial Bioenergetics. Cell Rep 25:1786-1799.e4 (2018). PubMed: 30428348