AB109527

重组Anti-TIM50抗体[EPR5784]

Anti-TIM50 antibody [EPR5784]

RabMAb
Recombinant
了解详情

Be the first to review this product! Submit a review

(4 Publications)

Rabbit Recombinant Monoclonal TIM50 antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 4 publications.

查看别名

TIM50, PRO1512, TIMM50, Mitochondrial import inner membrane translocase subunit TIM50

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TIM50 antibody [EPR5784] (AB109527)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TIM50 antibody [EPR5784] (AB109527)

ab109527 at 1/50 staining TIM50 in paraffin-embedded human liver tissue by Immunohistochemistry.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TIM50 antibody [EPR5784] (AB109527)

ab109527 at 1/50 staining TIM50 in paraffin-embedded human kidney tissue by Immunohistochemistry.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Unknown

Western blot - Anti-TIM50 antibody [EPR5784] (AB109527)

All lanes:

Western blot - Anti-TIM50 antibody [EPR5784] (ab109527) at 1/10000 dilution

Lane 1:

293T (Human embryonic kidney epithelial cell) cell lysate at 10 µg

Lane 2:

SH-SY5Y cell lysate at 10 µg

Lane 3:

HepG2 cell lysate at 10 µg

Lane 4:

HeLa cell lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 40 kDa

false

不同偶联物与剂型 (1)

Carrier free

Anti-TIM50 antibody [EPR5784] - BSA and Azide free

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR5784

亚型

IgG

不含载体蛋白

反应种属

Human

应用

WB, IHC-P

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

style

left-column

反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/50000", "WB-species-notes": "" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

style

left-column

产品详情

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Species reactivity
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species.
Please contact us for more information.

style

left-column

性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 0.1% BSA

运输条件

Blue Ice

储存信息

Stable for 12 months at -20°C

style

left-column

补充信息

This supplementary information is collated from multiple sources and compiled automatically.

TIM50 also known as Translocase of Inner Mitochondrial Membrane 50 is a protein weighing approximately 50 kDa. This protein plays an important mechanical role in the mitochondrial presequence translocase functioning as a receptor and import channel within the inner mitochondrial membrane. TIM50 is expressed predominantly within the inner membranes of mitochondria in eukaryotic cells.

Biological function summary

TIM50 facilitates the import of presequence-containing proteins from the cytosol into the mitochondrial matrix acting as part of the TIM23 complex. This complex orchestrates the translocation of proteins across the inner mitochondrial membrane. By interacting with presequences of nuclear-encoded mitochondrial proteins TIM50 ensures proper import and processing which is important for mitochondrial function and biogenesis.

Pathways

TIM50 is critical in the mitochondrial protein import pathway which is essential for mitochondrial respiration and energy production. TIM50 interacts with other proteins such as TIM23 and TIM17 to form the translocase of the inner membrane 23 (TIM23) complex. TIM50 also links to the mitochondrial unfolded protein response (UPR) where it may influence the regulation of proteostasis within mitochondria alongside proteins like HSP70.

Mutations and dysregulation of TIM50 have connections to mitochondrial diseases which encompass a range of disorders due to mitochondrial dysfunction. Additionally TIM50 may affect neurodegenerative diseases where mitochondrial impairment plays a role. Alterations in TIM50 influencing mitochondrial function might correlate with proteins like cytochrome c which can contribute to apoptotic pathways and cell death in these diseases.

style

left-column

style

left-column

产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

靶点信息

Essential component of the TIM23 complex, a complex that mediates the translocation of transit peptide-containing proteins across the mitochondrial inner membrane (PubMed : 30190335, PubMed : 38828998). Has some phosphatase activity in vitro; however such activity may not be relevant in vivo.. Isoform 2. May participate in the release of snRNPs and SMN from the Cajal body.

See full target information TIMM50

style

left-column

文献 (4)

Recent publications for all applications. Explore the full list and refine your search

Molecular carcinogenesis 62:293-302 PubMed36342357

2022

Dihydroartemisinin synergistically enhances the cytotoxic effects of oxaliplatin in colon cancer by targeting the PHB2-RCHY1 mediated signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Xiwei Wang,Yingying Zheng,Zhengbin Chai,Ji Li,Changhui Zhu,Yanling Peng,Juanjuan Qiu,Jiajun Xu,Chunyan Liu

Molecular carcinogenesis 58:767-776 PubMed30604908

2019

TIMM50 promotes tumor progression via ERK signaling and predicts poor prognosis of non-small cell lung cancer patients.

Applications

Unspecified application

Species

Unspecified reactive species

Xiupeng Zhang,Shuai Han,Haijing Zhou,Lin Cai,Jingduo Li,Nan Liu,Yang Liu,Liang Wang,Chuifeng Fan,Ailin Li,Yuan Miao

Cell reports 25:1786-1799.e4 PubMed30428348

2018

Human COX7A2L Regulates Complex III Biogenesis and Promotes Supercomplex Organization Remodeling without Affecting Mitochondrial Bioenergetics.

Applications

Unspecified application

Species

Unspecified reactive species

Teresa Lobo-Jarne,Eva Nývltová,Rafael Pérez-Pérez,Alba Timón-Gómez,Thibaut Molinié,Austin Choi,Arnaud Mourier,Flavia Fontanesi,Cristina Ugalde,Antoni Barrientos

Human molecular genetics 27:160-177 PubMed29112723

2017

In vitro and in vivo studies of the ALS-FTLD protein CHCHD10 reveal novel mitochondrial topology and protein interactions.

Applications

Unspecified application

Species

Unspecified reactive species

S R Burstein,F Valsecchi,H Kawamata,M Bourens,R Zeng,A Zuberi,T A Milner,S M Cloonan,C Lutz,A Barrientos,G Manfredi

View all publications

style

left-column

style

left-column

style

right-column

Abcam Product Promise

我们致力于为您的研究提供高质量的试剂，为您科研的每一步提供支持。若我们的产品未能达到预期性能，我们向您提供 Abcam Product Promise 保障。

详情请参阅我们的条款与条件。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

重组Anti-TIM50抗体[EPR5784]