AB251909

Anti-TIM22抗体

Name: TIM22抗体 (ab251909)| Abcam中文官网
Brand: Abcam Limited
SKU: AB251909
Price: 5947 CNY
Availability: InStock

Anti-TIM22 antibody

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(1 Publication)

Rabbit Polyclonal TIM22 antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human TIMM22 aa 50 to C-terminus.

查看别名

TEX4, TIM22, TIMM22, Mitochondrial import inner membrane translocase subunit Tim22, Testis-expressed protein 4

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TIM22 antibody (AB251909)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TIM22 antibody (AB251909)

Paraffin-embedded human adrenal gland tissue stained for TIM22 using ab251909 at 1/500 dilution in immunohistochemical analysis.

Unknown

Western blot - Anti-TIM22 antibody (AB251909)

All lanes:

Western blot - Anti-TIM22 antibody (ab251909) at 0.4 µg/mL

Lane 1:

Vector only transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate

Lane 2:

HEK-293T overexpressing TIM22 with a C terminal myc-DDK tag, whole cell lysate.

Predicted band size: 20 kDa

false

关键信息

宿主种属

Rabbit

克隆

Polyclonal

亚型

IgG

不含载体蛋白

反应种属

Human

应用

WB, IHC-P

applications

免疫原

Recombinant Fragment Protein within Human TIMM22 aa 50 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q9Y584

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500 - 1/1000", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Xenopus laevis": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Xenopus tropicalis": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Immunogen

存储溶液

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

The TIM22 protein also known as Translocase of Inner Mitochondrial Membrane 22 has a molecular mass of approximately 20 kilodaltons. It plays an important mechanical role in the import and insertion of membrane proteins into the mitochondrial inner membrane. TIM22 locates itself in the mitochondrial inner membrane where it functions as part of a larger translocase complex.

Biological function summary

TIM22 interacts with several mitochondria-rich cellular environments to facilitate the integration of polytopic proteins. As part of the TIM22 complex it integrates endeavored proteins into the mitochondrial inner membrane. This integration is essential for the optimal function of the mitochondria. It interacts closely with other components including TIM54 and TIM18 which assist in its function within the complex.

Pathways

The TIM22 complex activities heavily contribute to the mitochondrial import pathways. It remains connected with the translocase of outer mitochondrial membrane (TOM) complex as it receives incoming proteins. TIM22 connects with TOM40 and small TIM such as TIM9 and TIM10 during the import and sorting processes. This integration ensures that proteins achieve the correct membrane insertion needed for mitochondrial function.

TIM22-related dysfunction can lead to mitochondrial-related disorders such as mitochondrial myopathy. This happens when the protein integration into the mitochondrial membrane faces disruption. Additionally deficiencies or mutations in TIM22 have links to neurodegenerative disorders where proper mitochondrial function is compromised. The interaction with other members of the mitochondrial membrane translocase complexes like TIM54 and TIM18 is important in these disorder contexts.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Essential core component of the TIM22 complex, a complex that mediates the import and insertion of multi-pass transmembrane proteins into the mitochondrial inner membrane. In the TIM22 complex, it constitutes the voltage-activated and signal-gated channel. Forms a twin-pore translocase that uses the membrane potential as external driving force in 2 voltage-dependent steps (By similarity).

See full target information TIMM22

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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Cell journal 25:222-228 PubMed37210642

2023

Understanding The Regulatory Role of USP32 and SHMT2 in The Progression of Gastric Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Jun Li,Yafei Bo,Bo Ding,Lei Wang

View all publications

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Anti-TIM22抗体

Anti-TIM22 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TIM22 antibody (AB251909)

Western blot - Anti-TIM22 antibody (AB251909)