Anti-TIL/TLR1抗体(ab37068)
Key features and details
- Rabbit polyclonal to TIL/TLR1
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Mouse
- Isotype: IgG
概述
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产品名称
Anti-TIL/TLR1抗体
参阅全部 TIL/TLR1 一抗 -
描述
兔多克隆抗体to TIL/TLR1 -
宿主
Rabbit -
特异性
ab37068 recognises Toll-like receptor 1 (TIL/TLR1).
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经测试应用
适用于: WB, ICC/IF, IHC-Pmore details -
种属反应性
与反应: Mouse -
免疫原
Synthetic peptide corresponding to Human TIL/TLR1 (C terminal).
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阳性对照
- IHC-P: Mouse spleen lysate IF/ICC: RAW246.7 cell line. WB: Mouse spleen lysate.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. -
存储溶液
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading...
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纯度
Affinity purified -
纯化说明
Purified by ion exchange chromatography -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab37068于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 90 kDa.
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ICC/IF |
Use at an assay dependent concentration.
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IHC-P |
Use a concentration of 10 µg/ml.
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说明 |
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WB
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 90 kDa. |
ICC/IF
Use at an assay dependent concentration. |
IHC-P
Use a concentration of 10 µg/ml. |
靶标
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功能
Participates in the innate immune response to microbial agents. Cooperates with TLR2 to mediate the innate immune response to bacterial lipoproteins or lipopeptides. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. -
组织特异性
Ubiquitous. Highly expressed in spleen, ovary, peripheral blood leukocytes, thymus and small intestine. -
序列相似性
Belongs to the Toll-like receptor family.
Contains 12 LRR (leucine-rich) repeats.
Contains 1 LRRCT domain.
Contains 1 TIR domain. -
细胞定位
Cell membrane. Cytoplasmic vesicle > phagosome membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 21897 Mouse
- SwissProt: Q9EPQ1 Mouse
- Unigene: 273024 Mouse
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别名
- CD281 antibody
- CD281 antigen antibody
- KIAA0012 antibody
see all
图片
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Lane 1 : Anti-TIL/TLR1 antibody (ab37068) at 1 µg/ml
Lane 2 : Anti-TIL/TLR1 antibody (ab37068) at 2 µg/ml
Lane 3 : Anti-TIL/TLR1 antibody (ab37068) at 4 µg/ml
All lanes : Mouse spleen lysate
Lysates/proteins at 15 µg per lane.
Predicted band size: 90 kDa
Additional bands at: 75 kDa. We are unsure as to the identity of these extra bands. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TIL/TLR1 antibody (ab37068)
ab37068 at 10µg/ml staining TIL/TLR1 in mouse spleen tissue by IHC
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ICC/IF image of ab37068 stained RAW246.7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab37068, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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Immunofluorescence of TIL/TLR1 in Mouse Spleen cells using ab37068 at 20 ug/ml.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (3)
ab37068 被引用在 3 文献中.
- Zhu J et al. Engineered Lactococcus lactis secreting Flt3L and OX40 ligand for in situ vaccination-based cancer immunotherapy. Nat Commun 13:7466 (2022). PubMed: 36463242
- Zhou Y et al. Puerarin improves graft bone defect through microRNA-155-3p-mediated p53/TNF-a/STAT1 signaling pathway. Int J Mol Med 46:239-251 (2020). PubMed: 32377717
- Hinojosa E et al. Age-associated inflammation and toll-like receptor dysfunction prime the lungs for pneumococcal pneumonia. J Infect Dis 200:546-54 (2009). WB ; Mouse . PubMed: 19586419