AB290683

重组Anti-TIGIT抗体[EPR25804-72] - BSA and Azide free (Detector)

Anti-TIGIT antibody [EPR25804-72] - BSA and Azide free (Detector)

RabMAb
Recombinant
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Rabbit Recombinant Monoclonal TIGIT antibody. Carrier free. Suitable for sELISA and reacts with Human samples.

查看别名

VSIG9, VSTM3, TIGIT, T-cell immunoreceptor with Ig and ITIM domains, V-set and immunoglobulin domain-containing protein 9, V-set and transmembrane domain-containing protein 3

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Sandwich ELISA - Anti-TIGIT antibody [EPR25804-72] - BSA and Azide free (Detector) (AB290683)

sELISA

Supplier Data

Sandwich ELISA - Anti-TIGIT antibody [EPR25804-72] - BSA and Azide free (Detector) (AB290683)

Sandwich ELISA of ab290706 with the capture antibody dilution at 2 µg/mL and detector antibody dilution at 0.5 µg/mL.

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR25804-72

亚型

IgG

不含载体蛋白

Yes

反应种属

Human

应用

sELISA

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "sELISA" : {"fullname" : "Sandwich ELISA", "shortname":"sELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "sELISA-species-checked": "guaranteed", "sELISA-species-dilution-info": "", "sELISA-species-notes": "<p></p>" } } }

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产品详情

ab290683 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Human TIGIT. The recommended pair for sandwich ELISA is:
Capture: ab290682 , Human TIGIT Capture Antibody (unconjugated)
Detector: ab2906823, Human TIGIT Detector Antibody (unconjugated)
The reference range value is 5.86-375 pg/mL.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Sandwich ELISA
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

pH: 7.2 - 7.4 Constituents: PBS

运输条件

Blue Ice

补充信息

This supplementary information is collated from multiple sources and compiled automatically.

TIGIT also known as T cell immunoreceptor with Ig and ITIM domains is an immunoglobulin superfamily receptor. It weighs approximately 26 kDa. TIGIT commonly expresses on T cells especially on regulatory T cells (Tregs) and effector memory T cells. It also presents on natural killer (NK) cells. Among its alternate forms some notable ones also include the anti-TIGIT and biotinylated TIGIT antibodies used in experimental research to monitor immune responses.

Biological function summary

TIGIT acts as an inhibitory receptor modulating immune responses. It belongs to the CD28 family and binds to poliovirus receptor (PVR) and nectin-2 on antigen-presenting cells. This interaction inhibits T cell activation and proliferation and increases Treg cell activity maintaining immune homeostasis. TIGIT forms a complex with other inhibitory receptors like PD-1 2B4 and CD96 to further regulate immune functions.

Pathways

The interaction of TIGIT with its ligands significantly affects the immune checkpoint pathway. This involvement regulates T cell and NK cell activity. TIGIT is related to CD226 (DNAM-1) which competes for binding to its ligands influencing cellular signaling pathways. Through these pathways TIGIT signals through the downstream SHP-1 and SHP-2 proteins affecting immune cell signaling and response.

TIGIT has implications in cancer and autoimmune diseases. Its inhibitory function can allow tumor progression by dampening effective anti-tumor immunity. In cancer TIGIT’s activity often pairs with PD-1 both contributing to immune checkpoint blockade resistance necessary for tumor evasion. In autoimmune diseases increased TIGIT expression could result in reduced immune cell attack against self tissues showing its potential to modulate treatment strategies.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Inhibitory receptor that plays a role in the modulation of immune responses. Suppresses T-cell activation by promoting the generation of mature immunoregulatory dendritic cells (PubMed : 19011627). Upon binding to its ligands PVR/CD155 or NECTIN2/CD112, which are expressed on antigen-presenting cells, sends inhibitory signals to the T-cell or NK cell. Mechanistically, interaction with ligand leads to phosphorylation of the cytoplasmic tail by Src family tyrosine kinases such as FYN or LCK, allowing subsequent binding to adapter GRB2 and SHIP1/INPP5D. In turn, inhibits PI3K and MAPK signaling cascades (PubMed : 23154388). In addition, associates with beta-arrestin-2/ARRB2 to recruit SHIP1/INPP5D that suppresses autoubiquitination of TRAF6 and subsequently inhibits NF-kappa-B signaling pathway (PubMed : 24817116). Acts also as a receptor for NECTIN4 to inhibit NK cell cytotoxicity (PubMed : 32503945).

See full target information TIGIT

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Abcam Product Promise

我们致力于为您的研究提供高质量的试剂，为您科研的每一步提供支持。若我们的产品未能达到预期性能，我们向您提供 Abcam Product Promise 保障。

详情请参阅我们的条款与条件。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

重组Anti-TIGIT抗体[EPR25804-72] - BSA and Azide free (Detector)

Anti-TIGIT antibody [EPR25804-72] - BSA and Azide free (Detector)

Sandwich ELISA - Anti-TIGIT antibody [EPR25804-72] - BSA and Azide free (Detector) (AB290683)

关键信息

宿主种属

克隆

克隆号

亚型

不含载体蛋白

反应种属

应用

免疫原