重组Anti-TIGIT抗体[BLR047F] - BSA free (ab243903)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [BLR047F] to TIGIT - BSA free
- Suitable for: WB, IHC-P
- Reacts with: Human
概述
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产品名称
Anti-TIGIT抗体[BLR047F] - BSA free
参阅全部 TIGIT 一抗 -
描述
兔单克隆抗体[BLR047F] to TIGIT - BSA free -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-Pmore details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide within Human TIGIT aa 150 to the C-terminus (C terminal). The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements. NP_776160.2 and Gene ID 201633.
Database link: Q495A1 -
阳性对照
- IHC-P: Human tonsil tissue
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常规说明
This product is sold under License from Bethyl Laboratories, Inc.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.8
Preservative: 0.09% Sodium azide
Constituent: 99% Borate buffered saline -
Concentration information loading...
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纯化说明
Recombinant antibody was purified from cell culture supernatant. -
克隆
单克隆 -
克隆编号
BLR047F -
同种型
IgG -
研究领域
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab243903于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB |
1/1000.
|
|
IHC-P | (5) |
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
说明 |
---|
WB
1/1000. |
IHC-P
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
靶标
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功能
Binds with high affinity to the poliovirus receptor (PVR) which causes increased secretion of IL10 and decreased secretion of IL12B and suppresses T cell activation by promoting the generation of mature immunoregulatory dendritic cells. -
组织特异性
Expressed at low levels on peripheral memory and regulatory CD4+ T cells and NK cells and is up-regulated following activation of these cells (at protein level). -
序列相似性
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
结构域
Contains 1 copy of a cytoplasmic motif that is referred to as the immunoreceptor tyrosine-based inhibitor motif (ITIM). This motif is involved in modulation of cellular responses. The phosphorylated ITIM motif can bind the SH2 domain of several SH2-containing phosphatases. -
细胞定位
Cell membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 201633 Human
- Omim: 612859 Human
- SwissProt: Q495A1 Human
- Unigene: 421750 Human
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别名
- DKFZp667A205 antibody
- ENSMUSG00000071552 antibody
- FLJ39873 antibody
see all
图片
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Formalin-fixed, paraffin-embedded human tonsil tissue stained for TIGIT using ab243903 at 1/200 dilution in immunohistochemical analysis. A HRP-conjugated goat anti-rabbit antibody was used as the secondary. DAB staining.
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Western blot analysis using ab243903 at 1/1000 dilution.
Lane 1: HeLa cell whole cell lysate (50 µg).
Lane 2: HEK-293T cell whole cell lysate (50 µg).
Lane 3: MyLa CD4+ whole cell lysate (50 µg).
Lane 4: HepG2 whole cell lysate (50 µg).
Lane 5: MG whole cell lysate (50 µg).
Lane 6: Jurkat whole cell lysate (50 µg).
Lane 7: GaMG whole cell lysate (50 µg).
Lane 8: A549 whole cell lysate (50 µg).
A HRP-conjugated goat anti-rabbit IgG antibody was used as the secondary. Detection: chemiluminescence with an exposure time of 3 minutes. -
Immunohistochemical analysis of Formalin/PFA-fixed paraffin-embedded human tonsil tissue sections labeling TIGIT with ab243903 (red). Dylight 594 conjugated goat-anti-rabbit IgG was used as secondary antibody. Counterstain: DAPI (blue).
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Immunohistochemical analysis of Formalin/PFA-fixed paraffin-embedded human tonsil tissue sections labeling TIGIT with ab243903 at 1/250 (red). HRP-conjugated goat anti-rabbit IgG was used as secondary antibody. Counterstain: DAPI (blue).
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (16)
ab243903 被引用在 16 文献中.
- Guo C et al. TIGIT as a Novel Prognostic Marker for Immune Infiltration in Invasive Breast Cancer. Comb Chem High Throughput Screen 26:639-651 (2023). PubMed: 35770416
- Hao B et al. Single-cell RNA sequencing analysis revealed cellular and molecular immune profiles in lung squamous cell carcinoma. Transl Oncol 27:101568 (2023). PubMed: 36270103
- Ma J et al. The immune checkpoint expression in the tumor immune microenvironment of DLBCL: Clinicopathologic features and prognosis. Front Oncol 12:1069378 (2022). PubMed: 36561512
- Nakazawa T et al. Expression of T-cell immunoreceptor with immunoglobulin and tyrosine-based inhibitory motif domains (TIGIT) in anaplastic thyroid carcinoma. BMC Endocr Disord 22:204 (2022). PubMed: 35971106
- Huo X et al. Identification of Novel Immunologic Checkpoint Gene Prognostic Markers for Ovarian Cancer. J Oncol 2022:8570882 (2022). PubMed: 36157232