Anti-TIGAR抗体(ab62533)
Key features and details
- Rabbit polyclonal to TIGAR
- Suitable for: IHC-P, ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-TIGAR抗体
参阅全部 TIGAR 一抗 -
描述
兔多克隆抗体to TIGAR -
宿主
Rabbit -
经测试应用
适用于: IHC-P, ICC/IF, WBmore details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide (Human) of 19 amino acids from a region near the centre of TIGAR
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阳性对照
- MCF7 cell lysate Human brain tissue
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. -
存储溶液
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab62533于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P |
Use a concentration of 2.5 µg/ml.
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| ICC/IF |
Use a concentration of 20 µg/ml.
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| WB |
Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 30 kDa (predicted molecular weight: 30 kDa).
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| 说明 |
|---|
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IHC-P
Use a concentration of 2.5 µg/ml. |
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ICC/IF
Use a concentration of 20 µg/ml. |
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WB
Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 30 kDa (predicted molecular weight: 30 kDa). |
靶标
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功能
Probable fructose-biphosphatase. Lowers cellular levels of fructose 2,6-bisphosphate. Protects cells against reactive oxygen species and against apoptosis induced by p53/TP53. -
序列相似性
Belongs to the phosphoglycerate mutase family. -
翻译后修饰
Phosphorylated upon DNA damage, probably by ATM or ATR. - Information by UniProt
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数据库链接
- Entrez Gene: 57103 Human
- Omim: 610775 Human
- SwissProt: Q9NQ88 Human
- Unigene: 504545 Human
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别名
- 6-bisphosphatase TIGAR antibody
- C12ORF5 antibody
- chromosome 12 open reading frame 5 antibody
see all
图片
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Western blot - Anti-TIGAR antibody (ab62533)Lane 1 : Anti-TIGAR antibody (ab62533) at 0.5 µg/ml
Lane 2 : Anti-TIGAR antibody (ab62533) at 1 µg/ml
Lane 3 : Anti-TIGAR antibody (ab62533) at 2 µg/ml
All lanes : MCF7 cell lysate
Lysates/proteins at 15 µg per lane.
Predicted band size: 30 kDa
Observed band size: 30 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TIGAR antibody (ab62533)immunohistochemical analysis of TIGAR expression in human brain tissue using 2.5 µg/ml ab62533. -
Immunocytochemistry/ Immunofluorescence - Anti-TIGAR antibody (ab62533)Immunofluorescence of TIGAR in Human Brain cells using ab62533 at 20 ug/ml.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (7)
ab62533 被引用在 7 文献中.
- Chen F et al. TIGAR/AP-1 axis accelerates the division of Lgr5- reserve intestinal stem cells to reestablish intestinal architecture after lethal radiation. Cell Death Dis 11:501 (2020). PubMed: 32632140
- Roche ME et al. Translocase of the outer mitochondrial membrane complex subunit 20 (TOMM20) facilitates cancer aggressiveness and therapeutic resistance in chondrosarcoma. Biochim Biophys Acta Mol Basis Dis 1866:165962 (2020). PubMed: 32920118
- Wang H et al. Loss of TIGAR Induces Oxidative Stress and Meiotic Defects in Oocytes from Obese Mice. Mol Cell Proteomics 17:1354-1364 (2018). PubMed: 29776966
- Gooptu M et al. Mitochondrial and glycolytic metabolic compartmentalization in diffuse large B-cell lymphoma. Semin Oncol 44:204-217 (2017). PubMed: 29248132
- Tambe Y et al. The drs tumor suppressor regulates glucose metabolism via lactate dehydrogenase-B. Mol Carcinog 55:52-63 (2016). PubMed: 25620379
- Maiti AK et al. IL-4 Protects the Mitochondria Against TNFa and IFN? Induced Insult During Clearance of Infection with Citrobacter rodentium and Escherichia coli. Sci Rep 5:15434 (2015). IHC ; Mouse . PubMed: 26481427
- Kim J et al. TIGAR regulates glycolysis in ischemic kidney proximal tubules. Am J Physiol Renal Physiol 308:F298-308 (2015). PubMed: 25503731