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Cell Biology Apoptosis Nucleus Other

Anti-TIA1抗体[TIA-1] (ab2712)

  • Datasheet
  • SDS
Reviews (6)Q&A (5)References (16)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TIA1 antibody [TIA-1] (ab2712)

    Key features and details

    • Mouse monoclonal [TIA-1] to TIA1
    • Suitable for: IHC-P
    • Reacts with: Mouse, Human, Monkey
    • Isotype: IgG1

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    概述

    • 产品名称

      Anti-TIA1抗体[TIA-1]
      参阅全部 TIA1 一抗
    • 描述

      小鼠单克隆抗体[TIA-1] to TIA1
    • 宿主

      Mouse
    • 经测试应用

      适用于: IHC-Pmore details
    • 种属反应性

      与反应: Mouse, Human, Monkey
    • 免疫原

      Recombinant full length protein. This information is proprietary to Abcam and/or its suppliers.

    • 阳性对照

      • IHC-P: Human spleen tissue.
    • 常规说明

      TIA-1 (T-cell intracytoplasmic antigen) monoclonal antibody reacts with a 15 kDa cytoplasmic granule-associated protein, expressed in lymphocytes processing cytolytic potential. The expression of TIA-1 was studied in CD30+ anaplastic large cell lymphomas (ALCL), NK cell lymphomas and peripheral T-cell lymphomas and T cell lymphocytosis, B-cell lymphomas and lymphoblastic leukemia, Hodgkin’s etc. Studies showed that 60 to 70% of anaplastic large cell lymphoma reacted with TIA-1. Studies also indicate that TIA-1 reacts with most large granular lymphocytic leukemia’s, hepatosplenic T-cell lymphomas, intestinal T-cell lymphomas, NK-like T-cell lymphomas, NK-cell lymphomas, nasal T/NK-cell lymphomas, subcutaneous T-cell lymphomas, pulmonary angiocentric lymphomas of T or NK phenotype. The author’s concluded from TIA-1 studies that anaplastic large-cell lymphomas are cytotoxic T-or NK-cell neoplasms. All B-cell lymphomas, Hodgkin’s and lymphoblastic leukemias were negative for TIA-1.

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    性能

    • 形式

      Liquid
    • 存放说明

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
    • 存储溶液

      Preservative: 0.1% Sodium azide
      Constituent: Proprietary Solution
    • Concentration information loading...
    • 纯度

      Proprietary Purification
    • Primary antibody说明

      TIA-1 (T-cell intracytoplasmic antigen) monoclonal antibody reacts with a 15 kDa cytoplasmic granule-associated protein, expressed in lymphocytes processing cytolytic potential. The expression of TIA-1 was studied in CD30+ anaplastic large cell lymphomas (ALCL), NK cell lymphomas and peripheral T-cell lymphomas and T cell lymphocytosis, B-cell lymphomas and lymphoblastic leukemia, Hodgkin’s etc. Studies showed that 60 to 70% of anaplastic large cell lymphoma reacted with TIA-1. Studies also indicate that TIA-1 reacts with most large granular lymphocytic leukemia’s, hepatosplenic T-cell lymphomas, intestinal T-cell lymphomas, NK-like T-cell lymphomas, NK-cell lymphomas, nasal T/NK-cell lymphomas, subcutaneous T-cell lymphomas, pulmonary angiocentric lymphomas of T or NK phenotype. The author’s concluded from TIA-1 studies that anaplastic large-cell lymphomas are cytotoxic T-or NK-cell neoplasms. All B-cell lymphomas, Hodgkin’s and lymphoblastic leukemias were negative for TIA-1.
    • 克隆

      单克隆
    • 克隆编号

      TIA-1
    • 骨髓瘤

      unknown
    • 同种型

      IgG1
    • 轻链类型

      unknown
    • 研究领域

      • Cell Biology
      • Apoptosis
      • Nucleus
      • Other
      • Immunology
      • Adaptive Immunity
      • T Cells
      • Non-CD
      • Epigenetics and Nuclear Signaling
      • DNA / RNA
      • RNA Processing
      • Other
      • Cancer
      • Cell Death
      • Apoptosis
      • Nucleus
      • Other

    相关产品

    • Antibody Diluent

      • Universal antibody diluent (ab79995)
    • Compatible Secondaries

      • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
      • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
    • Isotype control

      • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)
    • Recombinant Protein

      • Recombinant Human TIA1 protein (ab131879)

    应用

    The Abpromise guarantee

    Abpromise™承诺保证使用ab2712于以下的经测试应用

    “应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

    应用 Ab评论 说明
    IHC-P (1)
    1/50 - 1/100.

    Incubate for 30-60 minutes at RT.Use heat-mediated antigen retrieval, an optional pepsin treatment can also improve the signal.

    说明
    IHC-P
    1/50 - 1/100.

    Incubate for 30-60 minutes at RT.Use heat-mediated antigen retrieval, an optional pepsin treatment can also improve the signal.

    靶标

    • 功能

      Involved in alternative pre-RNA splicing and regulation of mRNA translation by binding to AU-rich elements (AREs) located in mRNA 3' untranslated regions (3' UTRs). Possesses nucleolytic activity against cytotoxic lymphocyte target cells. May be involved in apoptosis.
    • 序列相似性

      Contains 3 RRM (RNA recognition motif) domains.
    • 细胞定位

      Cytoplasmic granule. Nucleus. Accumulates in cytoplasmic stress granules (SG) following cellular damage.
    • Target information above from: UniProt accession P31483 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • 数据库链接

      • Entrez Gene: 7072 Human
      • Entrez Gene: 21841 Mouse
      • Omim: 603518 Human
      • SwissProt: P31483 Human
      • SwissProt: P52912 Mouse
      • 别名

        • Cytotoxic granule associated RNA binding protein 1 antibody
        • Cytotoxic granule associated RNA binding protein antibody
        • mTIA-1 antibody
        • Nucleolysin TIA 1 isoform p40 antibody
        • Nucleolysin TIA-1 isoform p40 antibody
        • Nucleolysin TIA1 isoform p40 antibody
        • p40 TIA 1 antibody
        • p40-TIA-1 (containing p15-TIA-1) antibody
        • p40-TIA-1 antibody
        • RNA binding protein TIA 1 antibody
        • RNA binding protein TIA1 antibody
        • RNA-binding protein TIA-1 antibody
        • T-cell-restricted intracellular antigen-1 antibody
        • TIA 1 antibody
        • TIA 1 cytotoxic granule associated RNA binding protein antibody
        • Tia antibody
        • TIA-1 antibody
        • TIA1 antibody
        • TIA1 cytotoxic granule associated RNA binding protein antibody
        • TIA1 cytotoxic granule associated RNA binding protein like 1 antibody
        • TIA1 protein antibody
        • TIA1_HUMAN antibody
        • TIAL1 antibody
        • TIAR antibody
        • WDM antibody
        see all

      图片

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TIA1 antibody [TIA-1] (ab2712)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TIA1 antibody [TIA-1] (ab2712)

        Human normal spleen. Staining is observed in the cytoplasm. Left panel: with primary antibody at 1 ug/ml. Right panel: isotype control. Sections were stained using an automated system at room temperature: sections were rehydrated and antigen retrieved with buffers citrate EDTA pH 9.0. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked for 10 minutes (casein 0.25% in PBS) then incubated with primary antibody for 20 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.

      实验方案

      • Immunohistochemistry protocols

      Click here to view the general protocols

      数据表及文件

      • SDS download

      • Datasheet download

        Download

      文献 (16)

      发表研究结果有使用 ab2712?请让我们知道,以便我们可以引用本数据表中的参考文章。

      ab2712 被引用在 16 文献中.

      • Lennox AL  et al. Pathogenic DDX3X Mutations Impair RNA Metabolism and Neurogenesis during Fetal Cortical Development. Neuron 106:404-420.e8 (2020). PubMed: 32135084
      • Niu Z  et al. Myopathy With SQSTM1 and TIA1 Variants: Clinical and Pathological Features. Front Neurol 9:147 (2018). IHC-P ; Human . PubMed: 29599744
      • Ghosh S & Geahlen RL Stress Granules Modulate SYK to Cause Microglial Cell Dysfunction in Alzheimer's Disease. EBioMedicine 2:1785-98 (2015). WB, ICC/IF ; Mouse . PubMed: 26870803
      • van Gool IC  et al. POLE Proofreading Mutations Elicit an Antitumor Immune Response in Endometrial Cancer. Clin Cancer Res 21:3347-55 (2015). IHC-P ; Human . PubMed: 25878334
      • Tessier SN  et al. The involvement of mRNA processing factors TIA-1, TIAR, and PABP-1 during mammalian hibernation. Cell Stress Chaperones 19:813-25 (2014). PubMed: 24590458
      View all Publications for this product

      客户评价及客户问答

      Show All 评价 Q&A
      提交评价 提交问题

      1-10 of 11 Abreviews or Q&A

      Immunocytochemistry abreview for Anti-TIA1 antibody [TIA-1]

      Below Average
      Abreviews
      Abreviews
      Application
      Immunocytochemistry
      Sample
      Human Cultured Cells (neuroblastoma)
      Specification
      neuroblastoma
      Fixative
      Paraformaldehyde
      Permeabilization
      Yes - 0.1% Triton-X 100
      Blocking step
      goat serum,0.5%BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 25°C
      Read More
      The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

      Abcam user community

      Verified customer

      提交于 Oct 02 2012

      Western blot abreview for Anti-TIA1 antibody [TIA-1]

      Average
      Abreviews
      Abreviews
      Application
      Western blot
      Sample
      Spermophilus tridecemlineatus Tissue lysate - whole (Liver, Heart)
      Loading amount
      30 µg
      Specification
      Liver, Heart
      Gel Running Conditions
      Reduced Denaturing (15%)
      Blocking step
      PVA as blocking agent for 1 minute(s) · Concentration: 1µg/mL · Temperature: 25°C
      Read More
      The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

      Abcam user community

      Verified customer

      提交于 Aug 06 2009

      Immunocytochemistry/ Immunofluorescence abreview for Anti-TIA1 antibody [TIA-1]

      Below Average
      Abreviews
      Abreviews
      Application
      Immunocytochemistry/ Immunofluorescence
      Sample
      Human Cell (Renal proximal tubular epithelial cells)
      Specification
      Renal proximal tubular epithelial cells
      Fixative
      Paraformaldehyde
      Blocking step
      BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 21°C
      Read More
      The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

      Dr. Robert Jenkins

      Verified customer

      提交于 Apr 13 2009

      Immunocytochemistry/ Immunofluorescence abreview for Anti-TIA1 antibody [TIA-1]

      Excellent
      Abreviews
      Abreviews
      abreview image
      Application
      Immunocytochemistry/ Immunofluorescence
      Sample
      Monkey Cell (COS 7 cells)
      Specification
      COS 7 cells
      Fixative
      Methanol
      Blocking step
      BSA as blocking agent for 30 minute(s) · Concentration: 3
      Read More
      The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

      Mrs. Gabriela Elena Oprea

      Verified customer

      提交于 Aug 16 2006

      Immunocytochemistry/ Immunofluorescence abreview for Anti-TIA1 antibody [TIA-1]

      Below Average
      Abreviews
      Abreviews
      Application
      Immunocytochemistry/ Immunofluorescence
      Sample
      Rat Cell (primary cultured hippocampal neurons, COS)
      Specification
      primary cultured hippocampal neurons, COS
      Fixative
      Paraformaldehyde
      Blocking step
      Other as blocking agent for 30 minute(s) · Concentration: 10%
      Read More
      The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

      Abcam user community

      Verified customer

      提交于 Jun 05 2006

      Question

      Dear Sir/Madam,

      I forgot to add the following kit-

      Ab 2712 Mouse Monoclonal to TIA1 250 ul

      Can you please add it?

      How do I pay?

      Can you e mail us the certificates of analysis?

      Regards

      Read More

      Abcam community

      Verified customer

      Asked on Sep 25 2012

      Answer

      Thank you very much for your email.

      One vial of ab2712 has been added to your order, and you can pay for this order with the PO that you provided or with a credit card if you prefer. If you'd like to add a credit card to your order, please call our Customer Services team at 888-772-2226.

      We can provide certificates of compliance instead of certificates of analysis, so I've attached these CoC's to this email. Please note that the Bax antibody Ab33020 is on back-order so we are not able to include a lot number on the CoC, but please feel free to contact me once the shipment arrives and I can update the CoC to include the lot number you receive.

      I hope that this will be useful, but please let me know if you have any questions or need anything else and I'll be happy to help.

      Read More

      Abcam Scientific Support

      回复于 Sep 25 2012

      Question

      Hello,
      I have been using ab2712 for IHC and getting great results. I was just wondering which isoform of TIA-1 the antibody binds to or does it bind both? According to the UniProt website from the datasheet for the antibody (http://www.uniprot.org/uniprot/P31483#section_alternative), the two isofroms are as follows:
      Isoform Long (identifier: P31483-1)
      This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
      Isoform Short (identifier: P31483-2)
      The sequence of this isoform differs from the canonical sequence as follows:
      93-104: SSTVVSTQRSQD → N

      Read More

      Abcam community

      Verified customer

      Asked on Nov 01 2012

      Answer

      Thank you for contacting us.We have not teted the specificity, the only information we have available is that the TIA-1 antibody reaacts with a 15 kDa cytoplasmic granule-associated protein. Please let me know if you have any further questions.

      Read More

      Abcam Scientific Support

      回复于 Nov 01 2012

      Question

      I would like to test ab2712 for use in ICC

      Read More

      Abcam community

      Verified customer

      Asked on Sep 05 2012

      Answer

      DISCOUNT CODE: ***
      Expiration date: January 5th, 2012

      I am very pleased to hear you would like to accept our offer and test ab2712 in ICC. This code will give you: 1 free primary antibody before the expiration date. To redeem this offer, please submit an Abreview for ICC and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. Please remember that submission of the Abreview is sufficient for the discount code to become active.
      For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.

      Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

      Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

      The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

      Read More

      Abcam Scientific Support

      回复于 Sep 05 2012

      Question

      We found the expiry date given below was not written on the antibody labels. The customers are querying about this and I would like to have some letter mentioning the expiry dates of these two items. Thank you.

      Read More

      Abcam community

      Verified customer

      Asked on May 09 2006

      Answer

      Thank you for your enquiry. The antibody storage conditions are detailed on every datasheet under the "Storage instructions" section. We make the general recommend that should our antibodies stipulate storage at -20oC then they can be stored for up to 2 years at this temperature. Likewise we suggest that our antibodies can be stored at -80oC for 10 years. When an antibody can only be kept at 4oC we recommend that it is functional for up to 12 months. I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

      Read More

      Abcam Scientific Support

      回复于 May 11 2006

      Question

      ReviewID: 4120 AbID: 2712 Status: Submitted TIA1 antibody [TIA-1] (ab2712): Sample: Human Cell (HeLa) Application: Immunocytochemistry/Immunofluorescence Blocking step:: Serum as blocking agent for 0 hour(s) 15 minute(s)  Concentration: 1% Fixation:: Paraformaldehyde Antigen retrieval step:: None Incubation time:: 1 hour(s)  0 minute(s)  Positive control:: My samples Dilution: 1/100 Rating: Didn't work - I would not use it again Secondary antibody: Name:: Non-Abcam one was used: [competitor] Conjugation: Cy3 Dilution:: 1/500

      Read More

      Abcam community

      Verified customer

      Asked on Feb 07 2006

      Answer

      Thank you for taking the time to submit a review to our system. I am taking your comments seriously and would appreciate your assistance to enable me to look into the source of the problem in more detail. Could you fill out the questionnaire at the following link so I can examine the details of your protocol more closely? https://www.abcam.com/index.html?section=ihc&pageconfig=technical&intAbID=2712&mode=questionaire My first recommendation would be to try Anaplastic large cell lymphoma or tonsil as a positive control as stated on our datasheet. I noticed that you fixed with 4% paraformaldehyde, however, we do know with formalin fixation that you need to use antigen retrieval and since this is an intracellular protein that you will need to use a permeabilization. I would recommend permeabilizing with 0.25% Triton X-100 or 100 uM digitonin or 0.5% saponin or trying an alternative fixative such as acetone. I hope this information helps you and I look forward to receiving your reply.

      Read More

      Abcam Scientific Support

      回复于 Feb 08 2006

      1-10 of 11 Abreviews or Q&A

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