重组Anti-Thyroid Peroxidase/TPO抗体[EPR5380]

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thyroid Peroxidase/TPO antibody [EPR5380] (AB109383)

ab109383, at a 1/100 dilution, staining Thyroid Peroxidase/TPO in paraffin-embedded Human thyroid gland tissue

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

• mIHC

Lab

Multiplex immunohistochemistry - Anti-Thyroid Peroxidase/TPO antibody [EPR5380] (AB109383)

Fluorescence multiplex immunohistochemical analysis of Human thyroid gland tissue (Formalin/PFA-fixed paraffin-embedded sections). Panel A : merged staining of anti-Thyroglobulin (ab151539, magenta; Opal™690), anti-Thyroid Peroxidase/TPO (ab109383, green; Opal™520) and anti-PAX8 (ab227707, red; Opal™570) on human thyroid gland. Panel B : anti-Thyroid Peroxidase/TPO stained on cytoplasm of follicular epithelial cells. Panel C : anti-PAX8 stained on nucleus of follicular epithelial cells. Panel D : anti-Thyroglobulin stained on colloid. Opal Polymer HRP Ms + Rb was used as a secondary antibody. The section was incubated in three rounds of staining : in the order of ab151539 at 1/5000 dilution (0.167 μg/ml), ab109383 at 1/1500 dilution (0.072 μg/ml) , and ab227707 at 1/200 dilution (5.22 μg/ml) for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system. The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope. DAPI (blue) was used as a nuclear counter stain. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

• IP

Lab

Immunoprecipitation - Anti-Thyroid Peroxidase/TPO antibody [EPR5380] (AB109383)

Thyroid Peroxidase/TPO was immunoprecipitated from 0.35 mg Human thyroid whole cell lysate 10 μg with ab109383 at 1/100 dilution (2μg). VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/5000 dilution.

Lane 1 : Human thyroid whole cell lysate 10 μg

Lane 2 : ab109383 IP in Human thyroid whole cell lysate

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab109383 in Human thyroid whole cell lysate

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

All lanes:

Immunoprecipitation - Anti-Thyroid Peroxidase/TPO antibody [EPR5380] (ab109383)

Predicted band size: 103 kDa

Observed band size: 110 kDa

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• WB

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Western blot - Anti-Thyroid Peroxidase/TPO antibody [EPR5380] (AB109383)

All lanes:

Western blot - Anti-Thyroid Peroxidase/TPO antibody [EPR5380] (ab109383) at 1/1000 dilution

All lanes:

Human thyroid lysate at 10 µg

Predicted band size: 103 kDa,35 kDa,54 kDa

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