Anti-Thyroid Hormone Receptor beta抗体(ab53170)
Key features and details
- Rabbit polyclonal to Thyroid Hormone Receptor beta
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-Thyroid Hormone Receptor beta抗体
参阅全部 Thyroid Hormone Receptor beta 一抗 -
描述
兔多克隆抗体to Thyroid Hormone Receptor beta -
宿主
Rabbit -
经测试应用
适用于: WB, ICC/IFmore details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide derived from human Thyroid Hormone Receptor beta
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阳性对照
- LoVo nuclear lysate
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, PBS -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab53170于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/500 - 1/1000. Detects a band of approximately 53 kDa (predicted molecular weight: 53 kDa).
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| ICC/IF |
Use a concentration of 1 - 5 µg/ml.
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| 说明 |
|---|
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WB
1/500 - 1/1000. Detects a band of approximately 53 kDa (predicted molecular weight: 53 kDa). |
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ICC/IF
Use a concentration of 1 - 5 µg/ml. |
靶标
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功能
High affinity receptor for triiodothyronine. -
疾病相关
Defects in THRB are the cause of generalized thyroid hormone resistance (GTHR) [MIM:188570, 274300]. GTHR is transmitted as an autosomal dominant trait, but an autosomal recessive form also exists. The disease is characterized by goiter, abnormal mental functions, increased susceptibility to infections, abnormal growth and bone maturation, tachycardia and deafness. Affected individuals may also have attention deficit-hyperactivity disorders (ADHD) and language difficulties. GTHR patients also have high levels of circulating thyroid hormones (T3-T4), with normal or slightly elevated thyroid stimulating hormone (TSH).
Defects in THRB are the cause of selective pituitary thyroid hormone resistance (PRTH) [MIM:145650]; also known as familial hyperthyroidism due to inappropriate thyrotropin secretion. PRTH is a variant form of thyroid hormone resistance and is characterized by clinical hyperthyroidism, with elevated free thyroid hormones, but inappropriately normal serum TSH. Unlike GRTH, where the syndrome usually segregates with a dominant allele, the mode of inheritance in PRTH has not been established. -
序列相似性
Belongs to the nuclear hormone receptor family. NR1 subfamily.
Contains 1 nuclear receptor DNA-binding domain. -
结构域
Composed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 7068 Human
- Omim: 190160 Human
- SwissProt: P10828 Human
- Unigene: 187861 Human
- Unigene: 728126 Human
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别名
- Avian erythroblastic leukemia viral (v erb a) oncogene homolog 2 antibody
- C ERBA 2 antibody
- C ERBA BETA antibody
see all
图片
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Western blot - Anti-Thyroid Hormone Receptor beta antibody (ab53170)All lanes : Anti-Thyroid Hormone Receptor beta antibody (ab53170) at 1/500 dilution
Lane 1 : LoVo cell extracts minus immunising peptide
Lane 2 : LoVo cell extracts plus immunising peptide
Predicted band size: 53 kDa -
Immunocytochemistry/ Immunofluorescence - Anti-Thyroid Hormone Receptor beta antibody (ab53170)ICC/IF image of ab53170 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab53170, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab53170 被引用在 1 文献中.
- Perrotta C et al. The thyroid hormone triiodothyronine controls macrophage maturation and functions: protective role during inflammation. Am J Pathol 184:230-47 (2014). PubMed: 24215914