重组Anti-Thyroglobulin抗体[EPR9730] - BSA and Azide free (ab271919)
![Flow Cytometry (Intracellular) - Anti-Thyroglobulin antibody [EPR9730] - BSA and Azide free (ab271919)](https://www.abcam.cn/ps/products/271/ab271919/Images/ab271919-7-ab156008297986antithyroglobulinantibodyepr9730flowcytometry.jpg "Flow Cytometry (Intracellular) - Anti-Thyroglobulin antibody [EPR9730] - BSA and Azide free (ab271919)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR9730] to Thyroglobulin - BSA and Azide free
- Suitable for: ICC/IF, IHC-P, WB, Flow Cyt (Intra)
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-Thyroglobulin抗体[EPR9730] - BSA and Azide free
参阅全部 Thyroglobulin 一抗 -
描述
兔单克隆抗体[EPR9730] to Thyroglobulin - BSA and Azide free -
宿主
Rabbit -
经测试应用
适用于: ICC/IF, IHC-P, WB, Flow Cyt (Intra)more details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat
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免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- Mouse thyroid, rat thyroid, and Human thyroid lysates, Human thyroid gland follicular carcinoma tissue and Human thyroid gland papillary carcinoma tissue, TT cells
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常规说明
ab271919 is the carrier-free version of ab156008.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
存储溶液
pH: 7.2
Constituent: PBS -
无载体
是 -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR9730 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Conjugation kits
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab271919于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF |
Use at an assay dependent concentration.
|
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| IHC-P |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.
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| WB |
Use at an assay dependent concentration. Predicted molecular weight: 305 kDa.
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| Flow Cyt (Intra) |
Use at an assay dependent concentration.
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| 说明 |
|---|
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ICC/IF
Use at an assay dependent concentration. |
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IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.
|
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WB
Use at an assay dependent concentration. Predicted molecular weight: 305 kDa. |
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Flow Cyt (Intra)
Use at an assay dependent concentration. |
靶标
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功能
Precursor of the iodinated thyroid hormones thyroxine (T4) and triiodothyronine (T3). -
组织特异性
Thyroid gland specific. -
疾病相关
Defects in TG are the cause of congenital hypothyroidism due to dyshormonogenesis type 3 (CHDH3) [MIM:274700]. A disorder due to thyroid dyshormonogenesis, causing large goiters of elastic and soft consistency in the majority of patients. Although the degree of thyroid dysfunction varies considerably among patients with defective thyroglobulin synthesis, patients usually have a relatively high serum free triiodothyronine (T3) concentration with disproportionately low free tetraiodothyronine (T4) level. The maintenance of relatively high free T3 levels prevents profound tissue hypothyroidism except in brain and pituitary, which are dependent on T4 supply, resulting in neurologic and intellectual defects in some cases.
Variations in TG are associated with susceptibility to autoimmune thyroid disease type 3 (AITD3) [MIM:608175]. AITDs including Graves disease (GD) and Hashimoto thyroiditis (HT), are among the most common human autoimmune diseases. They are complex diseases, which are caused by an interaction between susceptibility genes and nongenetic factors, such as infection. -
序列相似性
Belongs to the type-B carboxylesterase/lipase family.
Contains 11 thyroglobulin type-1 domains. -
翻译后修饰
Sulfated tyrosines are desulfated during iodination. -
细胞定位
Secreted. - Information by UniProt
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数据库链接
- Entrez Gene: 7038 Human
- Entrez Gene: 21819 Mouse
- Entrez Gene: 24826 Rat
- Omim: 188450 Human
- SwissProt: P01266 Human
- SwissProt: O08710 Mouse
- SwissProt: P06882 Rat
- Unigene: 654591 Human
see all -
别名
- AITD 3 antibody
- AITD3 antibody
- hTG antibody
see all
图片
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Flow Cytometry (Intracellular) - Anti-Thyroglobulin antibody [EPR9730] - BSA and Azide free (ab271919)
Intracellular Flow Cytometry analysis of TT (Human thyroid carcinoma epithelial cell) cells labeling Thyroglobulin with purified ab156008 at 1/70 dilution (10 µg/ml) (red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488) secondary antibody was used at 1/2000 dilution. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab156008).
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![Immunocytochemistry/ Immunofluorescence - Anti-Thyroglobulin antibody [EPR9730] - BSA and Azide free (ab271919)](https://www.abcam.cn/ps/products/271/ab271919/Images/ab271919-6-ab156008297942antithyroglobulinantibodyepr9730immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Thyroglobulin antibody [EPR9730] - BSA and Azide free (ab271919)Immunocytochemistry/ Immunofluorescence analysis of TT (Human thyroid carcinoma epithelial cell) cells labeling Thyroglobulin with Purified ab156008 at 1:100 dilution. Cells were fixed in 100% Methanol. ab150077 Goat anti rabbit IgG(Alexa Fluor® 488) was used as the secondary antibody at 1:1000 dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab156008). -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thyroglobulin antibody [EPR9730] - BSA and Azide free (ab271919)](https://www.abcam.cn/ps/products/271/ab271919/Images/ab271919-5-ab156008297705antithyroglobulinantibodyepr9730immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thyroglobulin antibody [EPR9730] - BSA and Azide free (ab271919)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human thyroid carcinoma tissue sections labeling Thyroglobulin with Purified ab156008 at 1:500 dilution (1.36 μg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab156008). -
![Flow Cytometry (Intracellular) - Anti-Thyroglobulin antibody [EPR9730] - BSA and Azide free (ab271919)](https://www.abcam.cn/ps/products/271/ab271919/Images/ab271919-4-ab156008221099antithyroglobulinantibodyepr9730flowcytometry.jpg)
Flow Cytometry (Intracellular) - Anti-Thyroglobulin antibody [EPR9730] - BSA and Azide free (ab271919)This image is courtesy of an Abreview submitted by Sanjay GawadeUnpurified ab156008 staining Thyroglobulin in mouse thyroid cells by intracellular flow cytometry. Cells were fixed with formaldehyde and permeabilized with permeabilization buffer. The sample was incubated with the primary antibody (1/100 in FACS buffer) for 30 minutes at 24°C. An Alexa Fluor® 647-conjugated goat anti-rabbit IgG (1/2000) was used as the secondary antibody. Gating Strategy: Epithlial cells. Red line shows unlabeled sample, blue line shows labeled sample.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab156008).
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thyroglobulin antibody [EPR9730] - BSA and Azide free (ab271919)](https://www.abcam.cn/ps/products/271/ab271919/Images/ab271919-3-ab156008163347antithyroglobulinantibodyepr9730immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thyroglobulin antibody [EPR9730] - BSA and Azide free (ab271919)Immunohistochemical analysis of paraffin embedded Human thyroid gland follicular carcinoma tissue labeling Thyroglobulin with unpurified ab156008 antibody at 1/250.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab156008). -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thyroglobulin antibody [EPR9730] - BSA and Azide free (ab271919)](https://www.abcam.cn/ps/products/271/ab271919/Images/ab271919-2-ab156008163346antithyroglobulinantibodyepr9730immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thyroglobulin antibody [EPR9730] - BSA and Azide free (ab271919)Immunohistochemical analysis of paraffin embedded Human thyroid gland papillary carcinoma tissue labeling Thyroglobulin with unpurified ab156008 antibody at 1/250.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab156008). -
![Immunocytochemistry/ Immunofluorescence - Anti-Thyroglobulin antibody [EPR9730] - BSA and Azide free (ab271919)](https://www.abcam.cn/ps/products/271/ab271919/Images/ab271919-1-ab156008163345antithyroglobulinantibodyepr9730immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Thyroglobulin antibody [EPR9730] - BSA and Azide free (ab271919) -
![Anti-Thyroglobulin antibody [EPR9730] - BSA and Azide free (ab271919)](https://www.abcam.cn/ps/products/271/ab271919/Images/ab271919-8-benefits-of-recombinant-antibodies.png)
Anti-Thyroglobulin antibody [EPR9730] - BSA and Azide free (ab271919)
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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Datasheet download
Certificate of Compliance
文献 (0)
ab271919 尚未被引用在任何文献中。
