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AB308383

重组Anti-Thymidylate synthase抗体[HL1236]

Anti-Thymidylate synthase antibody [HL1236]

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Rabbit Recombinant Monoclonal Thymidylate Synthase antibody. Carrier free. Suitable for IHC-P, ICC/IF, WB and reacts with Cat, Dog, Human, Mouse, Rat samples. Immunogen corresponding to Recombinant Fragment Protein within Human TYMS.

查看别名

TS, OK/SW-cl.29, TYMS, Thymidylate synthase, TSase

4 Images
Immunocytochemistry/ Immunofluorescence - Anti-Thymidylate synthase antibody [HL1236] (AB308383)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Thymidylate synthase antibody [HL1236] (AB308383)

For immunofluorescence analysis, HeLa cells were fixed in 4% paraformaldehyde at room temperature for 15 minutes and permeabilized for detection of Thymidylate synthase protein using ab308383 at 1/200 dilution (green). Nuclei (blue) is stained with fluoroshield with DAPI. Alpha Tubulin stained by alpha Tubulin antibody (red) diluted at 1/1000

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thymidylate synthase antibody [HL1236] (AB308383)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thymidylate synthase antibody [HL1236] (AB308383)

Immunohistochemical analysis of paraffin-embedded Dog intestin and colon tissue labelling Thymidylate synthase with ab308383 at 1/100 dilution.

Antigen Retrieval : Citrate buffer, pH 6.0, 15 min

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thymidylate synthase antibody [HL1236] (AB308383)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thymidylate synthase antibody [HL1236] (AB308383)

Immunohistochemical analysis of paraffin-embedded cat brain tissue labelling Thymidylate synthase with ab308383 at 1/100 dilution.

Antigen Retrieval : Citrate buffer, pH 6.0, 15 min

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thymidylate synthase antibody [HL1236] (AB308383)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thymidylate synthase antibody [HL1236] (AB308383)

Immunohistochemical analysis of paraffin-embedded dog brain tissue labelling Thymidylate synthase with ab308383 at 1/100 dilution.

Antigen Retrieval : Citrate buffer, pH 6.0, 15 min

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

HL1236

亚型

IgG

不含载体蛋白

Yes

反应种属

Cat, Dog, Human, Mouse, Rat

应用

WB, IHC-P, ICC/IF

applications

免疫原

Recombinant Fragment Protein within Human TYMS. The exact immunogen used to generate this antibody is proprietary information.

P04818

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搭配使用产品

Assay Kits

AB288923

Human Thymidylate Synthase ELISA Kit

Recombinant|SimpleStep

Sandwich ELISA - Human Thymidylate Synthase ELISA Kit (AB288923)

  • 0
  • 0
Primary Antibodies

AB181602

Anti-GAPDH antibody [EPR16891] - Loading Control

RabMAb|Recombinant|Lab Essentials|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAPDH antibody [EPR16891] - Loading Control (AB181602)

  • 5
  • 23
Primary Antibodies

AB284861

Anti-Thymidine Phosphorylase antibody [EPR25629-129]

BOND RX™ Validated|20ul selling size|RabMAb|Recombinant|Trial Size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thymidine Phosphorylase antibody [EPR25629-129] (AB284861)

  • 0
  • 0
Primary Antibodies

AB124814

Anti-Dihydrofolate reductase (DHFR) antibody [EPR5285]

BOND RX™ Validated|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dihydrofolate reductase (DHFR) antibody [EPR5285] (AB124814)

  • 4
  • 1
Primary Antibodies

AB137114

Anti-RRM1 antibody [EPR8483]

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RRM1 antibody [EPR8483] (AB137114)

  • 5
  • 1
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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/200", "ICCIF-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>" }, "Rat": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>" }, "Cat": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>" }, "Dog": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>" } } }
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产品详情

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

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性能和储存信息

形式
Liquid
纯化工艺
Affinity purification Protein A
存储溶液
pH: 7.4 Constituents: PBS
运输条件
Blue Ice
推荐的短期储存时间
1-2 weeks
推荐的短期储存条件
+4°C
推荐的长期储存条件
+4°C
分装信息
Upon delivery aliquot
储存信息
Avoid freeze / thaw cycle
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Thymidylate Synthase (TS) also known as TYMS or TSase is an important enzyme involved in the de novo synthesis of thymidylate a nucleotide necessary for DNA replication and repair. TS catalyzes the methylation of deoxyuridine monophosphate (dUMP) to deoxythymidine monophosphate (dTMP) using 510-methylenetetrahydrofolate as a cofactor. It has an approximate molecular weight of 36 kDa. The enzyme is expressed in the cytoplasm of proliferating cells predominantly in tissues with high cellular turnover like bone marrow and the intestinal lining.
Biological function summary

Thymidylate synthase plays a critical role in DNA synthesis and cell division. As a member of the folate cycle it indirectly influences the supply of thymidine for DNA synthesis affecting cell proliferation. The enzyme generally functions as a homodimer which is necessary for its activity. Increased expression of TS usually correlates with high rates of cell division making it an important factor in rapidly growing tissues and tumors.

Pathways

Several important biological networks involve thymidylate synthase especially the folate and pyrimidine metabolism pathways. These pathways are essential for the maintenance of nucleotide pools within the cell. TS is functionally related to proteins like dihydrofolate reductase (DHFR) which also play roles in folate metabolism and DNA synthesis. Collectively these proteins ensure the proper balance of nucleotide precursors for DNA replication and repair.

Thymidylate synthase is closely associated with cancer and certain autoimmunity conditions. Overexpression of TS is commonly observed in various cancers as tumor cells require increased nucleotide synthesis for rapid proliferation. This makes TS a target for several anticancer drugs like 5-fluorouracil which inhibits TS activity. Additionally aberrations in enzymes like dihydropyrimidine dehydrogenase (DPD) could affect TS function leading to chemotherapeutic agent sensitivity in cancer treatments.
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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:
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靶点信息

Catalyzes the reductive methylation of 2'-deoxyuridine 5'-monophosphate (dUMP) to thymidine 5'-monophosphate (dTMP), using the cosubstrate, 5,10- methylenetetrahydrofolate (CH2H4folate) as a 1-carbon donor and reductant and contributes to the mitochondrial and nuclear de novo thymidylate biosynthesis pathway.
See full target information TYMS
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