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Epigenetics and Nuclear Signaling DNA / RNA DNA Synthesis Other

Anti-Thymidylate Synthase抗体(ab7398)

  • Datasheet
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Western blot - Anti-Thymidylate Synthase antibody (ab7398)

    Key features and details

    • Sheep polyclonal to Thymidylate Synthase
    • Suitable for: WB
    • Reacts with: Human
    • Isotype: IgG

    选择批间可重复性更高的重组抗体

    Product image
    Anti-Thymidylate Synthase antibody [EPR4545] (ab108995)
    • 研究可靠 —— 各批次间结果一致且可重复
    • 长期批量供应 —— 采用重组技术,可实现快速生产
    • 首次实验即可成功 —— 经过大量验证确认了特异性
    • 符合伦理标准 —— 产品不含动物成分

    概述

    • 产品名称

      Anti-Thymidylate Synthase抗体
      参阅全部 Thymidylate Synthase 一抗
    • 描述

      羊多克隆抗体to Thymidylate Synthase
    • 宿主

      Sheep
    • 特异性

      In general, this antibody can detect antigen in a variety of human cells and tissues, as well as bacteria, African green monkey, rat and mouse. Somewhat lower dilutions may be required in some non-human cell lines. Anti-Thymidylate Synthase can detect Thymidylate Synthase by immunochemistry in proliferating cell cultures and tissues but does not stain nonproliferating cells. Normal colon mucosa shows weak staining; however, some colorectal cancer specimens show very strong staining.

    • 经测试应用

      适用于: WBmore details
    • 种属反应性

      与反应: Human
      不与反应: Saccharomyces cerevisiae
    • 免疫原

      Recombinant full length protein corresponding to Human Thymidylate Synthase. Produced in E.coli.
      Database link: P04818

    • 常规说明

      The antibody recognizes the expected additional band corresponding to the ternary complex of human TS-dFUMP-reduced folate in HeLa cells treated with the TS inhibitor 5-FUdR. This event occurs in most human breast, colorectal, gastric, head and neck carcinomas. The antibody recognizes 36 kDa human TS.

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    性能

    • 形式

      Liquid
    • 存放说明

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
    • 存储溶液

      Preservative: 0.01% Sodium azide
      Constituents: 0.424% Potassium phosphate solution, 0.88% Sodium chloride
    • Concentration information loading...
    • 克隆

      多克隆
    • 同种型

      IgG
    • 研究领域

      • Epigenetics and Nuclear Signaling
      • DNA / RNA
      • DNA Synthesis
      • Other
      • Metabolism
      • Pathways and Processes
      • Metabolic signaling pathways
      • Nucleotide metabolism
      • Purine and pyrimidine synthesis

    相关产品

    • Isotype control

      • Sheep IgG - Isotype Control (ab37385)
    • Recombinant Protein

      • Recombinant Human Thymidylate Synthase protein (ab95378)

    应用

    The Abpromise guarantee

    Abpromise™承诺保证使用ab7398于以下的经测试应用

    “应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

    应用 Ab评论 说明
    WB
    1/500 - 1/2000. Predicted molecular weight: 37 kDa.
    说明
    WB
    1/500 - 1/2000. Predicted molecular weight: 37 kDa.

    靶标

    • 功能

      Contributes to the de novo mitochondrial thymidylate biosynthesis pathway.
    • 通路

      Pyrimidine metabolism; dTTP biosynthesis.
    • 序列相似性

      Belongs to the thymidylate synthase family.
    • 细胞定位

      Nucleus. Cytoplasm. Mitochondrion. Mitochondrion matrix. Mitochondrion inner membrane.
    • Target information above from: UniProt accession P04818 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • 数据库链接

      • Entrez Gene: 7298 Human
      • Omim: 188350 Human
      • SwissProt: P04818 Human
      • Unigene: 369762 Human
      • 别名

        • d TMP synthase antibody
        • EC 2.1.1.45 antibody
        • HsT422 antibody
        • MGC88736 antibody
        • OTTHUMP00000162195 antibody
        • Thymidylate synthase antibody
        • Thymidylate synthetase antibody
        • TMS antibody
        • TS antibody
        • TSase antibody
        • Tyms antibody
        • TYMS protein antibody
        • Tyms thymidylate synthetase antibody
        • TYSY_HUMAN antibody
        see all

      图片

      • Western blot - Anti-Thymidylate Synthase antibody (ab7398)
        Western blot - Anti-Thymidylate Synthase antibody (ab7398)
        ab7398 is shown to detect thymidylate synthase present in a HeLa cell extract. Each lane is estimated to contain 4 µg of protein. Lanes 1, 2 and 3 represent 1/2000, 1/5000 and 1/10000 fold dilutions of the antibody. Detection was made using HRP Rabbit-a-Sheep IgG diluted 1/1000 and color development using TMB substrate for approximately 4'.

      实验方案

      • Western blot protocols

      Click here to view the general protocols

      数据表及文件

      • Datasheet download

        Download

      文献 (7)

      发表研究结果有使用 ab7398?请让我们知道,以便我们可以引用本数据表中的参考文章。

      ab7398 被引用在 7 文献中.

      • Zhang L  et al. Disrupted folate metabolism with anesthesia leads to myelination deficits mediated by epigenetic regulation of ERMN. EBioMedicine 43:473-486 (2019). PubMed: 31060905
      • Gu YJ  et al. GRP78 confers the resistance to 5-FU by activating the c-Src/LSF/TS axis in hepatocellular carcinoma. Oncotarget 6:33658-74 (2015). WB . PubMed: 26378040
      • MacFarlane AJ  et al. Azoxymethane-induced colon carcinogenesis in mice occurs independently of de novo thymidylate synthesis capacity. J Nutr 144:419-24 (2014). PubMed: 24500934
      • Macfarlane AJ  et al. Nuclear localization of de novo thymidylate biosynthesis pathway is required to prevent uracil accumulation in DNA. J Biol Chem 286:44015-22 (2011). WB ; Mouse . PubMed: 22057276
      • Gay S  et al. Nucleotide supply, not local histone acetylation, sets replication origin usage in transcribed regions. EMBO Rep 11:698-704 (2010). WB ; Chinese hamster . PubMed: 20671737
      • Fox JT  et al. A UV-responsive internal ribosome entry site enhances serine hydroxymethyltransferase 1 expression for DNA damage repair. J Biol Chem 284:31097-108 (2009). WB, IP ; Human . PubMed: 19734144
      • Woeller CF  et al. Evidence for small ubiquitin-like modifier-dependent nuclear import of the thymidylate biosynthesis pathway. J Biol Chem 282:17623-31 (2007). PubMed: 17446168

      客户评价及客户问答

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      1-6 of 6 Abreviews or Q&A

      Question

      Thank you very much for Proforma invoice. We will inform our customer and after then we will send an official order. But Hthe customer would like to know, if POSITIVE CINTROL is possible to buy from Abcam.

      Read More

      Abcam community

      Verified customer

      Asked on May 14 2004

      Answer

      Thank you for your enquiry. For ab7398 and 3145 - you can use 5-FU-resistant cancer cell lines or human breast, colorectal, gastric, head and neck carcinomas as positive controls. All the cells especially the rapidly proliferating cells express this enzyme. For details of our human tumor cell lysate, please take a look at our on-line catalogue: https://www.abcam.com/index.html?pageconfig=catalog_byproducttype&intProductTypeID=12&intProductSubTypeID=3

      Read More

      Abcam Scientific Support

      回复于 May 17 2004

      Question

      Please would you let me know the subclass of the antibody to thymidylate synthase and whether the light chain is kappa or lambda. Also, what is the sensitivity of the antibody.

      Read More

      Abcam community

      Verified customer

      Asked on Sep 25 2001

      Answer

      This is a polyclonal antibody, and so we cannot refer to subclasses. The concentration is 10mg/ml and the sensitivity can be seen from the recommended concentrations for each application.

      Read More

      Abcam Scientific Support

      回复于 Sep 27 2001

      Question

      Is the antibody directed to the N-terminal?

      Read More

      Abcam community

      Verified customer

      Asked on Aug 30 2001

      Answer

      This antibody was raised against the full length recombinant protien. The epitopes recognised by this antibody have not been mapped.

      Read More

      Abcam Scientific Support

      回复于 Aug 31 2001

      Question

      Would you please let me know what is the carrier protein for the antibody.

      Read More

      Abcam community

      Verified customer

      Asked on Aug 30 2001

      Answer

      This antibody was raised against a recombinant protein. A carrier protein was not used for immunisation.

      Read More

      Abcam Scientific Support

      回复于 Aug 31 2001

      Question

      Do you have an example of the results obtained for a Western Blot for this antibody. Also the quote is for 1 mg of antibody. Is this 1 mg of an IgG fraction or for purified antibody?

      Read More

      Abcam community

      Verified customer

      Asked on Jul 26 2001

      Answer

      The antibody is an IgG fraction. Unfortunatly we do not have an example to hand of the Western blot. However, the antibody works very well in Western blotting and we recommend this antibody for this application.

      Read More

      Neil Hayward

      Abcam Scientific Support

      回复于 Aug 16 2001

      Question

      Does your antibody (THymidylate Synthase) work on paraffin?

      Read More

      Abcam community

      Verified customer

      Asked on Jul 23 2001

      Answer

      Yes, the anti-hTS does work on paraffin.

      Read More

      Neil Hayward

      Abcam Scientific Support

      回复于 Jul 30 2001

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com

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