Anti-Thymidylate Synthase抗体(ab7398)
Key features and details
- Sheep polyclonal to Thymidylate Synthase
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术，可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-Thymidylate Synthase抗体
参阅全部 Thymidylate Synthase 一抗 -
描述
羊多克隆抗体to Thymidylate Synthase -
宿主
Sheep -
特异性
In general, this antibody can detect antigen in a variety of human cells and tissues, as well as bacteria, African green monkey, rat and mouse. Somewhat lower dilutions may be required in some non-human cell lines. Anti-Thymidylate Synthase can detect Thymidylate Synthase by immunochemistry in proliferating cell cultures and tissues but does not stain nonproliferating cells. Normal colon mucosa shows weak staining; however, some colorectal cancer specimens show very strong staining.
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经测试应用
适用于: WBmore details -
种属反应性
与反应: Human
不与反应: Saccharomyces cerevisiae -
免疫原
Recombinant full length protein corresponding to Human Thymidylate Synthase. Produced in E.coli.
Database link: P04818 -
常规说明
The antibody recognizes the expected additional band corresponding to the ternary complex of human TS-dFUMP-reduced folate in HeLa cells treated with the TS inhibitor 5-FUdR. This event occurs in most human breast, colorectal, gastric, head and neck carcinomas. The antibody recognizes 36 kDa human TS.The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.01% Sodium azide
Constituents: 0.424% Potassium phosphate solution, 0.88% Sodium chloride -
Concentration information loading... -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Isotype control
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Recombinant Protein
应用
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/500 - 1/2000. Predicted molecular weight: 37 kDa.
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| 说明 |
|---|
|
WB
1/500 - 1/2000. Predicted molecular weight: 37 kDa. |
靶标
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功能
Contributes to the de novo mitochondrial thymidylate biosynthesis pathway. -
通路
Pyrimidine metabolism; dTTP biosynthesis. -
序列相似性
Belongs to the thymidylate synthase family. -
细胞定位
Nucleus. Cytoplasm. Mitochondrion. Mitochondrion matrix. Mitochondrion inner membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 7298 Human
- Omim: 188350 Human
- SwissProt: P04818 Human
- Unigene: 369762 Human
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别名
- d TMP synthase antibody
- EC 2.1.1.45 antibody
- HsT422 antibody
see all
图片
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Western blot - Anti-Thymidylate Synthase antibody (ab7398)ab7398 is shown to detect thymidylate synthase present in a HeLa cell extract. Each lane is estimated to contain 4 µg of protein. Lanes 1, 2 and 3 represent 1/2000, 1/5000 and 1/10000 fold dilutions of the antibody. Detection was made using HRP Rabbit-a-Sheep IgG diluted 1/1000 and color development using TMB substrate for approximately 4'.
数据表及文件
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Datasheet download
文献 (7)
ab7398 被引用在 7 文献中.
- Zhang L et al. Disrupted folate metabolism with anesthesia leads to myelination deficits mediated by epigenetic regulation of ERMN. EBioMedicine 43:473-486 (2019). PubMed: 31060905
- Gu YJ et al. GRP78 confers the resistance to 5-FU by activating the c-Src/LSF/TS axis in hepatocellular carcinoma. Oncotarget 6:33658-74 (2015). WB . PubMed: 26378040
- MacFarlane AJ et al. Azoxymethane-induced colon carcinogenesis in mice occurs independently of de novo thymidylate synthesis capacity. J Nutr 144:419-24 (2014). PubMed: 24500934
- Macfarlane AJ et al. Nuclear localization of de novo thymidylate biosynthesis pathway is required to prevent uracil accumulation in DNA. J Biol Chem 286:44015-22 (2011). WB ; Mouse . PubMed: 22057276
- Gay S et al. Nucleotide supply, not local histone acetylation, sets replication origin usage in transcribed regions. EMBO Rep 11:698-704 (2010). WB ; Chinese hamster . PubMed: 20671737
- Fox JT et al. A UV-responsive internal ribosome entry site enhances serine hydroxymethyltransferase 1 expression for DNA damage repair. J Biol Chem 284:31097-108 (2009). WB, IP ; Human . PubMed: 19734144
- Woeller CF et al. Evidence for small ubiquitin-like modifier-dependent nuclear import of the thymidylate biosynthesis pathway. J Biol Chem 282:17623-31 (2007). PubMed: 17446168
