Anti-Thymidine Phosphorylase抗体- N-terminal (ab180783)
Key features and details
- Rabbit polyclonal to Thymidine Phosphorylase - N-terminal
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
概述
-
产品名称
Anti-Thymidine Phosphorylase抗体- N-terminal
参阅全部 Thymidine Phosphorylase 一抗 -
描述
兔多克隆抗体to Thymidine Phosphorylase - N-terminal -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-P, ICC/IFmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat -
免疫原
Recombinant full length protein corresponding to Human Thymidine Phosphorylase aa 1-482 (N terminal).
Database link: P19971 -
阳性对照
- A549 and THP1 cell lysates.
-
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 50% Glycerol -
Concentration information loading...
-
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
-
Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab180783于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB | (1) |
1/500 - 1/2000. Predicted molecular weight: 50 kDa.
|
IHC-P |
1/50 - 1/200.
|
|
ICC/IF |
Use at an assay dependent concentration.
|
说明 |
---|
WB
1/500 - 1/2000. Predicted molecular weight: 50 kDa. |
IHC-P
1/50 - 1/200. |
ICC/IF
Use at an assay dependent concentration. |
靶标
-
功能
May have a role in maintaining the integrity of the blood vessels. Has growth promoting activity on endothelial cells, angiogenic activity in vivo and chemotactic activity on endothelial cells in vitro.
Catalyzes the reversible phosphorolysis of thymidine. The produced molecules are then utilized as carbon and energy sources or in the rescue of pyrimidine bases for nucleotide synthesis. -
通路
Pyrimidine metabolism; dTMP biosynthesis via salvage pathway; dTMP from thymine: step 1/2. -
疾病相关
Mitochondrial DNA depletion syndrome 1 (MTDPS1) [MIM:603041]: A multisystem disease associated with mitochondrial dysfunction. It is clinically characterized by onset between the second and fifth decades of life, ptosis, progressive external ophthalmoplegia, gastrointestinal dysmotility (often pseudoobstruction), diffuse leukoencephalopathy, cachexia, peripheral neuropathy, and myopathy. Note=The disease is caused by mutations affecting the gene represented in this entry. -
序列相似性
Belongs to the thymidine/pyrimidine-nucleoside phosphorylase family. - Information by UniProt
-
数据库链接
- Entrez Gene: 1890 Human
- Entrez Gene: 72962 Mouse
- Entrez Gene: 315219 Rat
- Omim: 131222 Human
- SwissProt: P19971 Human
- SwissProt: Q99N42 Mouse
- SwissProt: Q5FVR2 Rat
- Unigene: 180903 Human
see all -
别名
- ECGF 1 antibody
- ECGF antibody
- ECGF1 antibody
see all
图片
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human stomach cancer tissue labelling Thymidine Phosphorylase with ab180783 at 1/100. Magnification: 200x.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human stomach tissue labelling Thymidine Phosphorylase with ab180783 at 1/100. Magnification: 200x.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung cancer tissue labelling Thymidine Phosphorylase with ab180783 at 1/100. Magnification: 200x.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney cancer tissue labelling Thymidine Phosphorylase with ab180783 at 1/100. Magnification: 200x.
-
Immunocytochemistry/Immunofluorescence analysis of HeLa cells using ab180783. Blue DAPI for nuclear staining.
-
All lanes : Anti-Thymidine Phosphorylase antibody - N-terminal (ab180783) at 1/500 dilution
Lane 1 : A549 cell lysate
Lane 2 : THP1 cell lysate
Predicted band size: 50 kDa
实验方案
数据表及文件
-
SDS download
-
Datasheet download
文献 (2)
ab180783 被引用在 2 文献中.
- Wang Q et al. A predictive signature for oxaliplatin and 5-fluorouracil based chemotherapy in locally advanced gastric cancer. Transl Oncol 14:100901 (2021). PubMed: 33091827
- Belcher A et al. Targeting Thymidine Phosphorylase With Tipiracil Hydrochloride Attenuates Thrombosis Without Increasing Risk of Bleeding in Mice. Arterioscler Thromb Vasc Biol 41:668-682 (2021). PubMed: 33297751