JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB307255

重组Anti-Thymidine Phosphorylase抗体[EPR25629-2] - BSA and Azide free (Detector)

Anti-Thymidine Phosphorylase antibody [EPR25629-2] - BSA and Azide free (Detector)

Be the first to review this product! Submit a review

|

(0 Publication)

Rabbit Recombinant Monoclonal Thymidine Phosphorylase antibody. Carrier free. Suitable for sELISA and reacts with Human samples.

查看别名

ECGF1, TYMP, Thymidine phosphorylase, TP, Gliostatin, Platelet-derived endothelial cell growth factor, TdRPase, PD-ECGF

1 Images
Sandwich ELISA - Anti-Thymidine Phosphorylase antibody [EPR25629-2] - BSA and Azide free (Detector) (AB307255)
  • sELISA

Supplier Data

Sandwich ELISA - Anti-Thymidine Phosphorylase antibody [EPR25629-2] - BSA and Azide free (Detector) (AB307255)

Sandwich ELISA of ab307253 with the capture antibody dilution at 2 µg/mL and detector antibody dilution at 0.5 µg/mL.

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR25629-2

亚型

IgG

不含载体蛋白

Yes

反应种属

Human

应用

sELISA

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

搭配使用产品

Assay Kits

AB324131

Human Thymidine phosphorylase (PD-ECGF) ELISA Kit- Extracellular

Recombinant|SimpleStep

Sandwich ELISA - Human Thymidine phosphorylase (PD-ECGF) ELISA Kit- Extracellular (AB324131)

  • 0
  • 0
Primary Antibodies

AB242995

Anti-Haptoglobin antibody [EPR20341-34] - BSA and Azide free (Detector)

RabMAb|Recombinant

Sandwich ELISA - Anti-Haptoglobin antibody [EPR20341-34] - BSA and Azide free (Detector) (AB242995)

  • 0
  • 0
Primary Antibodies

AB282259

Anti-Glutathione Peroxidase 4 antibody [EPR24463-10] - BSA and Azide free (Detector)

RabMAb|Recombinant

Sandwich ELISA - Anti-Glutathione Peroxidase 4 antibody [EPR24463-10] - BSA and Azide free (Detector) (AB282259)

  • 0
  • 0
Primary Antibodies

AB309215

Anti-ISG15 antibody [EPR24482-22] - BSA and Azide free (Detector)

RabMAb|Recombinant

Sandwich ELISA - Anti-ISG15 antibody [EPR24482-22] - BSA and Azide free (Detector) (AB309215)

  • 0
  • 0
Primary Antibodies

AB277667

Anti-LAMP1 antibody [EPR24395-49] - BSA and Azide free (Detector)

RabMAb|Recombinant

Sandwich ELISA - Anti-LAMP1 antibody [EPR24395-49] - BSA and Azide free (Detector) (AB277667)

  • 0
  • 0
style
left-column

反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "sELISA" : {"fullname" : "Sandwich ELISA", "shortname":"sELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "sELISA-species-checked": "testedAndGuaranteed", "sELISA-species-dilution-info": "", "sELISA-species-notes": "<p></p>" } } }
style
left-column

产品详情

Sandwich ELISA
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

style
left-column

性能和储存信息

形式
Liquid
纯化工艺
Affinity purification Protein A
存储溶液
pH: 7.2 - 7.4 Constituents: PBS
运输条件
Blue Ice
推荐的短期储存条件
+4°C
推荐的长期储存条件
+4°C
style
left-column

补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Thymidine phosphorylase also known as platelet-derived endothelial cell growth factor (PD-ECGF) acts mechanically to catalyze the reversible phosphorolysis of thymidine into thymine and 2-deoxy-alpha-D-ribose-1-phosphate. With a mass of approximately 44 kDa this enzyme is present in various tissues including blood vessels and macrophages. Its expression tends to be higher in tumor tissues which suggests a specific role in cancer biology.
Biological function summary

The enzyme participates in pyrimidine nucleoside metabolism providing an alternate route for thymidine catabolism. It is not part of a larger complex functioning independently to promote angiogenesis. Thymidine phosphorylase enhances the degradation of extracellular thymidine which can positively impact nucleotide balance within cells aiding DNA synthesis and repair.

Pathways

Thymidine phosphorylase operates significantly within the nucleotide salvage pathways. It is essential for clarifying thymidine availability for DNA synthesis. The enzyme links with enzymes like dihydropyrimidine dehydrogenase in pyrimidine degradation playing a critical role in maintaining cellular nucleotide homeostasis.

Altered activity of thymidine phosphorylase correlates with cancer and mitochondrial neurogastrointestinal encephalomyopathy (MNGIE). In tumors increased levels of this enzyme facilitate angiogenesis contributing to tumor growth and metastasis. In MNGIE mutations in this enzyme lead to imbalances in nucleotides causing mitochondrial dysfunction. The enzyme's dysregulation connects to angiogenesis-related proteins like VEGF in cancer highlighting potential therapeutic targets.
style
left-column
style
left-column

产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

靶点信息

May have a role in maintaining the integrity of the blood vessels. Has growth promoting activity on endothelial cells, angiogenic activity in vivo and chemotactic activity on endothelial cells in vitro.. Catalyzes the reversible phosphorolysis of thymidine. The produced molecules are then utilized as carbon and energy sources or in the rescue of pyrimidine bases for nucleotide synthesis.
See full target information TYMP
style
left-column
style
left-column
style
right-column

Abcam Product Promise

我们致力于为您的研究提供高质量的试剂，为您科研的每一步提供支持。若我们的产品未能达到预期性能，我们向您提供 Abcam Product Promise 保障。
详情请参阅我们的条款与条件。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com